Construct: sgRNA BRDN0001147875
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCCTGAATCGTATTCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK2B (2185)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76388
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
27436299 |
+ |
PTK2B |
NNNCCTGAATCGTATTCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
11056231 |
+ |
SRM |
NNNCCTGAATCCCATTCTTG |
NGG |
2 |
0.418 |
Tier II |
| 3 |
NC_000013.11 |
13 |
77149570 |
+ |
MYCBP2 |
NNNCCTGAATCCTTTTCTTG |
NGG |
2 |
0.2824 |
Tier II |
| 4 |
NC_000006.12 |
6 |
160524998 |
- |
LOC124901454 |
NNNCCTGCATAGTATTCTTG |
NGG |
2 |
0.3214 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
131812411 |
- |
Ptpru |
NNNCCTGAATCGTATTCTTT |
NGG |
1 |
0.7 |
Tier II |
| 2 |
NC_000070.6 |
4 |
104996101 |
+ |
Fyb2 |
NNNCCGGAATAGTATTCTTG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000076.6 |
10 |
80771602 |
+ |
Dot1l |
NNNCCAGAATCGTATTCCTG |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000068.7 |
2 |
152752037 |
- |
Gm32057 |
NNNCCTGAATCGTATTCTAG |
NGA |
2 |
0.0198 |
Tier III |
Other clones with same target sequence:
(none)
