Construct: sgRNA BRDN0001147876
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCCGCCATGAGAATGTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK3 (5595)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75967
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
30121878 |
- |
MAPK3 |
NNNCGCCATGAGAATGTCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
154317037 |
- |
GALNT10 |
NNNCACCATGAGAATGTCAT |
NAG |
2 |
0.2247 |
Tier II |
| 3 |
NC_000015.10 |
15 |
66761900 |
- |
SMAD6 |
NNNGGCCATGTGAATGTCAT |
NGG |
2 |
0.1538 |
Tier II |
| 4 |
NC_000022.11 |
22 |
27867329 |
+ |
PITPNB |
NNNAGCCATGAGAAGGTCAT |
NGG |
2 |
0.0421 |
Tier II |
| 5 |
NC_000010.11 |
10 |
64038181 |
+ |
LOC124902439 |
NNNCTCCTTGAGAATGTCAT |
NGG |
2 |
0.1286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
126760860 |
+ |
Mapk3 |
NNNCGCCATGAGAATGTTAT |
NGG |
1 |
0.6429 |
Tier I |
| 2 |
NC_000074.6 |
8 |
123134584 |
+ |
Cpne7 |
NNNCGCCATGAGGATGTCAG |
NGG |
2 |
0.1151 |
Tier I |
| 3 |
NC_000069.6 |
3 |
14098326 |
+ |
Ralyl |
NNNCACCATGAGAATGTCAT |
NGG |
1 |
0.8667 |
Tier II |
| 4 |
NC_000069.6 |
3 |
14164349 |
- |
Ralyl |
NNNCACCATGAGAATGTCAT |
NGG |
1 |
0.8667 |
Tier II |
| 5 |
NC_000071.6 |
5 |
121472082 |
- |
Tmem116 |
NNNTGCCATGAGAATGTCAT |
NGG |
1 |
0.8 |
Tier II |
| 6 |
NC_000071.6 |
5 |
143086182 |
- |
Rnf216 |
NNNCGCTATGAGTATGTCAT |
NGG |
2 |
0.2438 |
Tier II |
| 7 |
NC_000082.6 |
16 |
48618992 |
- |
Morc1 |
NNNGGCCATGAGAATGTCAT |
NCG |
2 |
0.0536 |
Tier II |
| 8 |
NC_000068.7 |
2 |
143591515 |
- |
Pcsk2 |
NNNCGCCATGAGCATGTGAT |
NGG |
2 |
0.0281 |
Tier II |
| 9 |
NC_000082.6 |
16 |
78117287 |
+ |
Gm5675 |
NNNCGCCATGAGAATGTCAT |
NGG |
0 |
1.0 |
Tier III |
| 10 |
NC_000069.6 |
3 |
14098326 |
+ |
Gm18661 |
NNNCACCATGAGAATGTCAT |
NGG |
1 |
0.8667 |
Tier III |
| 11 |
NC_000069.6 |
3 |
14164349 |
- |
Gm18659 |
NNNCACCATGAGAATGTCAT |
NGG |
1 |
0.8667 |
Tier III |
| 12 |
NC_000071.6 |
5 |
121472082 |
- |
Gm15546 |
NNNTGCCATGAGAATGTCAT |
NGG |
1 |
0.8 |
Tier III |
| 13 |
NC_000078.6 |
12 |
118793687 |
- |
Gm6171 |
NNNTGCTATGAGAATGTCAT |
NGG |
2 |
0.65 |
Tier III |
| 14 |
NC_000076.6 |
10 |
3327470 |
+ |
Gm29826 |
NNNCCCCATGAGACTGTCAT |
NGG |
2 |
0.1684 |
Tier III |
| 15 |
NC_000079.6 |
13 |
57353297 |
+ |
Gm33294 |
NNNTGCCATGAGAATGTCAC |
NGG |
2 |
0.0727 |
Tier III |
Other clones with same target sequence:
(none)
