Construct: sgRNA BRDN0001147879

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAACTTACCATAGACATCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C3 (5289)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76966

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 41993333 - PIK3C3 NNNCTTACCATAGACATCTG NGG 0 1.0 Tier I
2 NC_000002.12 2 196058063 + DNAH7 NNNATTACCATAGACAGCTG NGG 2 0.0991 Tier I
3 NC_000002.12 2 196051196 + DNAH7 NNNGTTACCATAGACAGCTG NGG 2 0.0588 Tier I
4 NC_000007.14 7 36616389 + AOAH NNNCTTACCAAAGACATCTG NGA 2 0.0521 Tier I
5 NC_000018.10 18 74653145 - ZNF407 NNNCTTACCACAGACATCTA NGG 2 0.625 Tier II
6 NC_000009.12 9 93072745 - SUSD3 NNNTTTACCAAAGACATCTG NGG 2 0.6 Tier II
7 NC_000011.10 11 99494666 - CNTN5 NNNCTCACCATAGTCATCTG NGG 2 0.4848 Tier II
8 NC_000023.11 X 47864764 + ZNF81 NNNCTTACCATAGGCATCTA NGG 2 0.4375 Tier II
9 NC_000017.11 17 48076265 - CBX1 NNNTTTTCCATAGACATCTG NGG 2 0.35 Tier II
10 NC_000006.12 6 43271571 - TTBK1 NNNCTAACCAGAGACATCTG NGG 2 0.3467 Tier II
11 NC_000002.12 2 142004875 - LRP1B NNNCTTAACATAGACAACTG NGG 2 0.3467 Tier II
12 NC_000023.11 X 104773521 + IL1RAPL2 NNNCTTACCATAGGCATATG NGG 2 0.2513 Tier II
13 NC_000003.12 3 189612873 + TP63 NNNCTTGCCCTAGACATCTG NGG 2 0.2353 Tier II
14 NC_000011.10 11 9813130 - SBF2 NNNCTTACCATATACATCTT NGG 2 0.21 Tier II
15 NC_000012.12 12 2616928 + CACNA1C NNNCTTACCACAGCCATCTG NGG 2 0.1429 Tier II
16 NC_000001.11 1 110217060 + KCNC4 NNNCTTACCATACACATGTG NGG 2 0.0561 Tier II
17 NC_000003.12 3 131643229 + CPNE4 NNNCTTGCCATAGAGATCTG NGG 2 0.0353 Tier II
18 NC_000003.12 3 104515784 - LOC105374021 NNNCTTAACATAGACATCTT NGG 2 0.455 Tier III
19 NC_000004.12 4 131846856 - LOC105377425 NNNCTTACCATTGAAATCTG NGG 2 0.0741 Tier III
20 NC_000009.12 9 39972524 - LOC105376050 NNNCTTACCATTGAAATCTG NGG 2 0.0741 Tier III
21 NC_000009.12 9 41490999 + LOC105376065 NNNCTTACCATTGAAATCTG NGG 2 0.0741 Tier III
22 NC_000009.12 9 62278196 + LOC105379263 NNNCTTACCATTGAAATCTG NGG 2 0.0741 Tier III
23 NC_000012.12 12 91991590 + LINC01619 NNNCTTACCATAGCCATGTG NGG 2 0.0286 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 30293122 - Pik3c3 NNNCTTACCATAGACATTTG NGG 1 0.6429 Tier I
2 NC_000079.6 13 20917146 - Aoah NNNCTTACCACAGACATCTG NGA 2 0.0463 Tier I
3 NC_000076.6 10 76546851 - Lss NNNCTTACCAAAGACCTCTG NGG 2 0.0 Tier I
4 NC_000076.6 10 74553222 - Pcdh15 NNNCTTGCTATAGACATCTG NGG 2 0.6176 Tier II
5 NC_000083.6 17 81577976 + Slc8a1 NNNTTTACCATATACATCTG NGG 2 0.24 Tier II
6 NC_000079.6 13 9408985 - Dip2c NNNCTTACCATAGGCATCAG NGG 2 0.1333 Tier II
7 NC_000073.6 7 57219643 + Gabrg3 NNNCTTACCATAGACATGTA NGG 2 0.125 Tier II
8 NC_000077.6 11 81532830 + Asic2 NNNCTTACCATACACATGTG NGG 2 0.0561 Tier II
9 NC_000073.6 7 128753400 - Sec23ip NNNCTTACCATACACCTCTG NGG 2 0.0 Tier II
10 NC_000072.6 6 143632748 + 1700060C16Rik NNNCTTGCCTTAGACATCTG NGG 2 0.6228 Tier III
11 NC_000082.6 16 71857609 - Gm18550 NNNCTTACCATAGTCATCTT NGG 2 0.3733 Tier III
12 NC_000068.7 2 78389374 - Gm46750 NNNCTTACCATAGATATCTG NGG 1 0.0667 Tier III
13 NC_000076.6 10 76546851 - 4930483K19Rik NNNCTTACCAAAGACCTCTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)