Construct: sgRNA BRDN0001147881

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATGCCAGAAGAATCCAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRK2 (120892)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77333

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 40263807 + LRRK2 NNNGCCAGAAGAATCCAGGG NGG 0 1.0 Tier I
2 NC_000001.11 1 8992700 + SLC2A7 NNNACCAGAAGAATCCAGGG NAG 2 0.2333 Tier I
3 NC_000007.14 7 143443836 - TAS2R60 NNNGCCAGAAGAAGACAGGG NGG 2 0.0 Tier I
4 NC_000001.11 1 166908686 + ILDR2 NNNACCAGAAGAATCCAGGG NGG 1 0.9 Tier II
5 NC_000015.10 15 86280297 + AGBL1 NNNGCTAGAAGGATCCAGGG NGG 2 0.6706 Tier II
6 NC_000012.12 12 112507682 - PTPN11 NNNGTCAGAAGAATCCAAGG NGG 2 0.4406 Tier II
7 NC_000017.11 17 8074675 - ALOX12B NNNGCCAGAGGGATCCAGGG NGG 2 0.4012 Tier II
8 NC_000012.12 12 130366490 + PIWIL1 NNNGCCTGAAGAATCCAGGT NGG 2 0.3062 Tier II
9 NC_000004.12 4 181658486 - TENM3 NNNGCCAGAAGAGTCCAGCG NGG 2 0.2924 Tier II
10 NC_000011.10 11 46356672 + DGKZ NNNGCCAGAAGCATCCAGGG NGG 1 0.2632 Tier II
11 NC_000005.10 5 37668650 + WDR70 NNNGCCAGAAGATTCCAGAG NGG 2 0.2143 Tier II
12 NC_000010.11 10 34551623 + PARD3 NNNGCGAGAAGAATCCAGGC NGG 2 0.2143 Tier II
13 NC_000008.11 8 30455980 + RBPMS NNNGCCAGTAGAACCCAGGG NGG 2 0.1714 Tier II
14 NC_000019.10 19 13276105 + CACNA1A NNNGCCAGAGGACTCCAGGG NGG 2 0.117 Tier II
15 NC_000019.10 19 38218836 + DPF1 NNNGCCAGAAGAAACCGGGG NGG 2 0.1092 Tier II
16 NC_000005.10 5 179116751 + ADAMTS2 NNNGCAAGAAGAATGCAGGG NGG 2 0.0464 Tier II
17 NC_000009.12 9 110372870 + SVEP1 NNNGTCAGAAGAATTCAGGG NGG 2 0.0424 Tier II
18 NC_000016.10 16 78222983 + WWOX NNNGCCAGAGGAATGCAGGG NGG 2 0.0278 Tier II
19 NC_000021.9 21 39380715 - GET1 NNNGCCAGAAGAGTCCAGGG NTG 2 0.0254 Tier II
20 NC_000021.9 21 39380715 - GET1-SH3BGR NNNGCCAGAAGAGTCCAGGG NTG 2 0.0254 Tier II
21 NC_000008.11 8 138689957 + COL22A1 NNNGCCAGAAGAATGCAGGC NGG 2 0.0214 Tier II
22 NC_000018.10 18 80196727 - PARD6G NNNTCCAGAAGAATCCAGGG NTG 2 0.0142 Tier II
23 NC_000003.12 3 47439962 - SCAP NNNGGCAGAAGAATCCAGGG NGT 2 0.0097 Tier II
24 NC_000002.12 2 217965803 + TNS1 NNNGCCAGAAGCAGCCAGGG NGG 2 0.0 Tier II
25 NC_000003.12 3 49486125 + DAG1 NNNGCCAGCAGAAGCCAGGG NGG 2 0.0 Tier II
26 NC_000021.9 21 39636415 + B3GALT5 NNNGCCAGGAGAAGCCAGGG NGG 2 0.0 Tier II
27 NC_000017.11 17 10772982 - TMEM220-AS1 NNNGCCAGAAGAATCAAGAG NGG 2 0.7143 Tier III
28 NC_000005.10 5 161931551 + LINC01202 NNNGACAGAAAAATCCAGGG NGG 2 0.5714 Tier III
29 NC_000004.12 4 18683508 - LOC105374510 NNNGGCAGAAGAATCCAGGA NGG 2 0.5625 Tier III
30 NC_000021.9 21 28135483 - LINC01697 NNNGCCAGAAGAATCCAGCA NGG 2 0.4203 Tier III
31 NC_000021.9 21 28135483 - LINC01695 NNNGCCAGAAGAATCCAGCA NGG 2 0.4203 Tier III
32 NC_000012.12 12 115629512 + LOC105370002 NNNGCCGGAATAATCCAGGG NGG 2 0.2715 Tier III
33 NC_000012.12 12 115629512 + LOC105370003 NNNGCCGGAATAATCCAGGG NGG 2 0.2715 Tier III
34 NC_000024.10 Y 20738673 - TBL1YP1 NNNGCCAGAAGAGCCCAGGG NGG 2 0.1863 Tier III
35 NC_000022.11 22 23690296 - GUSBP11 NNNGCCGGAAGACTCCAGGG NGG 2 0.1486 Tier III
36 NC_000022.11 22 32384220 - LOC339666 NNNGCCGGAAGACTCCAGGG NGG 2 0.1486 Tier III
37 NC_000010.11 10 117751152 - LINC02674 NNNGGCAGAAGAATTCAGGG NGG 2 0.04 Tier III
38 NC_000002.12 2 145230318 - LOC100505498 NNNGCCAGAAGAATGCAGGG NGC 2 0.0011 Tier III
39 NC_000007.14 7 143443836 - EPHA1-AS1 NNNGCCAGAAGAAGACAGGG NGG 2 0.0 Tier III
40 NC_000017.11 17 72479271 - LINC00673 NNNGCCAGAAGAAGGCAGGG NGG 2 0.0 Tier III
41 NC_000020.11 20 52539688 + LOC105372666 NNNGCCAGAAGAATCCAGGG NCA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 67205684 + Chmp1b NNNGCCAGAAGAATCAAGGG NTG 2 0.039 Tier I
2 NC_000080.6 14 72460602 + Gm9195 NNNGGCAGAAGAAGCCAGGG NGG 2 0.0 Tier I
3 NC_000074.6 8 110704432 + Vac14 NNNGCCAGAAGAATCCAAGA NGG 2 0.649 Tier II
4 NC_000068.7 2 83846014 + Fam171b NNNGCTAGAAGAGTCCAGGG NGG 2 0.6056 Tier II
5 NC_000085.6 19 7104666 - Macrod1 NNNGCCAGAGAAATCCAGGG NGG 2 0.5556 Tier II
6 NC_000085.6 19 7104666 - Flrt1 NNNGCCAGAGAAATCCAGGG NGG 2 0.5556 Tier II
7 NC_000072.6 6 138394442 - Lmo3 NNNGCCAGAAGATTCCAGGG NGG 1 0.3 Tier II
8 NC_000083.6 17 26284385 + Luc7l NNNGCCAGAAGTATCCAGAG NGG 2 0.2381 Tier II
9 NC_000070.6 4 106934338 + Ssbp3 NNNGCCAGAAGATTCCAGAG NGG 2 0.2143 Tier II
10 NC_000076.6 10 94832915 + Plxnc1 NNNGCCAGAGGTATCCAGGG NGG 2 0.1852 Tier II
11 NC_000077.6 11 32643249 - Fbxw11 NNNGCCAGAGGAATCCAGGG NAG 2 0.144 Tier II
12 NC_000084.6 18 67205684 + Gnal NNNGCCAGAAGAATCAAGGG NTG 2 0.039 Tier II
13 NC_000068.7 2 128592480 + Spdye4c NNNCCCAGAAGAATGCAGGG NGG 2 0.0265 Tier II
14 NC_000071.6 5 121317349 + Hectd4 NNNTCCAGAAGAATTCAGGG NGG 2 0.0242 Tier II
15 NC_000068.7 2 153238621 - Plagl2 NNNGCCAGAAGATTCCAGGG NTG 2 0.0117 Tier II
16 NC_000067.6 1 192638852 + Hhat NNNGGCAGAAGAAGCCAGGG NGG 2 0.0 Tier II
17 NC_000075.6 9 113297051 + Gm36367 NNNGCCAGCAGAATCCAAGG NGG 2 0.3956 Tier III
18 NC_000071.6 5 32726815 - Gm2420 NNNGCCAGAGGAGTCCAGGG NGG 2 0.3623 Tier III
19 NC_000080.6 14 62128995 + Gm35419 NNNGCCAGAAGATTCAAGGG NGG 2 0.3 Tier III
20 NC_000077.6 11 25863062 + 5730522E02Rik NNNCCCAGAAGAATACAGGG NGG 2 0.1176 Tier III
21 NC_000067.6 1 91615196 - Gm28380 NNNTCCAGAAGACTCCAGGG NGG 2 0.0766 Tier III
22 NC_000071.6 5 91898537 + Gm36357 NNNGCCAGAAGAATCCAGGG NCT 2 0.0 Tier III
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Other clones with same target sequence:

(none)