Construct: sgRNA BRDN0001147882
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGAACAGAGGGATGTCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAPSS1 (9061)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77612
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
107687080 |
- |
PAPSS1 |
NNNGAACAGAGGGATGTCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
47264456 |
- |
GABRB1 |
NNNGAACAGAGGGATGTTAA |
NGG |
1 |
0.6429 |
Tier II |
| 3 |
NC_000017.11 |
17 |
42420696 |
+ |
CAVIN1 |
NNNGAACAGAGGGATGCCAG |
NGG |
2 |
0.5398 |
Tier II |
| 4 |
NC_000009.12 |
9 |
130616017 |
+ |
FUBP3 |
NNNGAACAGAGAGATGTAAA |
NGG |
2 |
0.5026 |
Tier II |
| 5 |
NC_000018.10 |
18 |
13436636 |
- |
LDLRAD4 |
NNNGAAAAAAGGGATGTCAA |
NGG |
2 |
0.4821 |
Tier II |
| 6 |
NC_000010.11 |
10 |
17416516 |
- |
ST8SIA6 |
NNNGAAAAGAGGGAAGTCAA |
NGG |
2 |
0.4342 |
Tier II |
| 7 |
NC_000002.12 |
2 |
173243507 |
+ |
MAP3K20 |
NNNGAACAGGGGGATGCCAA |
NGG |
2 |
0.3922 |
Tier II |
| 8 |
NC_000006.12 |
6 |
166693922 |
- |
RPS6KA2 |
NNNGGACAGAGGGATGTCTA |
NGG |
2 |
0.3877 |
Tier II |
| 9 |
NC_000020.11 |
20 |
42721305 |
+ |
PTPRT |
NNNGAACAAGGGGATGTCAA |
NGG |
2 |
0.3571 |
Tier II |
| 10 |
NC_000021.9 |
21 |
29698222 |
- |
GRIK1 |
NNNGAACAGAGGGATGAAAA |
NGG |
2 |
0.2872 |
Tier II |
| 11 |
NC_000017.11 |
17 |
44798069 |
+ |
GJC1 |
NNNGAACCGAGGGAAGTCAA |
NGG |
2 |
0.2481 |
Tier II |
| 12 |
NC_000014.9 |
14 |
100065549 |
+ |
EVL |
NNNGGACAGAGGGATGTCAA |
NAG |
2 |
0.1867 |
Tier II |
| 13 |
NC_000001.11 |
1 |
205571626 |
+ |
MFSD4A |
NNNAAACAGAGGGATGTCCA |
NGG |
2 |
0.1862 |
Tier II |
| 14 |
NC_000006.12 |
6 |
44138460 |
- |
TMEM63B |
NNNGAACAGAGGGCTGTCAG |
NGG |
2 |
0.1639 |
Tier II |
| 15 |
NC_000012.12 |
12 |
3206409 |
+ |
TSPAN9 |
NNNGAACACAGGGCTGTCAA |
NGG |
2 |
0.1154 |
Tier II |
| 16 |
NC_000008.11 |
8 |
142258928 |
+ |
TSNARE1 |
NNNGAACAGAGGGGTGTCCA |
NGG |
2 |
0.0966 |
Tier II |
| 17 |
NC_000008.11 |
8 |
98019643 |
+ |
MATN2 |
NNNGAACAGAGGGATGTCAT |
NCG |
2 |
0.0643 |
Tier II |
| 18 |
NC_000017.11 |
17 |
44321411 |
+ |
SLC25A39 |
NNNGGACAGAGGGAGGTCAA |
NGG |
2 |
0.036 |
Tier II |
| 19 |
NC_000006.12 |
6 |
116631366 |
- |
RSPH4A |
NNNGAACAGGGGGATGTCAA |
NGC |
2 |
0.0123 |
Tier II |
| 20 |
NC_000002.12 |
2 |
42400425 |
- |
KCNG3 |
NNNGAACAGAGGGATGGCAA |
NTG |
2 |
0.0046 |
Tier II |
| 21 |
NC_000014.9 |
14 |
63528399 |
+ |
PPP2R5E |
NNNGAACAGAGGGATTTCAC |
NGG |
2 |
0.0 |
Tier II |
| 22 |
NC_000012.12 |
12 |
109236255 |
- |
ACACB |
NNNGAACAGAGGGCTCTCAA |
NGG |
2 |
0.0 |
Tier II |
| 23 |
NC_000002.12 |
2 |
173243507 |
+ |
MAP3K20-AS1 |
NNNGAACAGGGGGATGCCAA |
NGG |
2 |
0.3922 |
Tier III |
| 24 |
NC_000002.12 |
2 |
138607201 |
- |
LINC02631 |
NNNGGAGAGAGGGATGTCAA |
NGG |
2 |
0.3388 |
Tier III |
| 25 |
NC_000002.12 |
2 |
121926145 |
+ |
LOC105373592 |
NNNTAAGAGAGGGATGTCAA |
NGG |
2 |
0.1711 |
Tier III |
| 26 |
NC_000006.12 |
6 |
116631366 |
- |
LOC124901386 |
NNNGAACAGGGGGATGTCAA |
NGC |
2 |
0.0123 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
131583185 |
+ |
Papss1 |
NNNGAGCAGAGGGATGTCAA |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000078.6 |
12 |
72493456 |
+ |
Lrrc9 |
NNNGAGCAGAGGGAAGTCAA |
NGG |
2 |
0.4135 |
Tier II |
| 3 |
NC_000075.6 |
9 |
32029245 |
+ |
Arhgap32 |
NNNGGACAGAGGGATGACAA |
NGG |
2 |
0.384 |
Tier II |
| 4 |
NC_000076.6 |
10 |
22700012 |
- |
Slc2a12 |
NNNGAACAAAGGGATGACAA |
NGG |
2 |
0.3429 |
Tier II |
| 5 |
NC_000076.6 |
10 |
89162363 |
+ |
Ano4 |
NNNGAACAGAGGGGTGTAAA |
NGG |
2 |
0.2513 |
Tier II |
| 6 |
NC_000073.6 |
7 |
29335457 |
- |
Sipa1l3 |
NNNCACCAGAGGGATGTCAA |
NGG |
2 |
0.2406 |
Tier II |
| 7 |
NC_000070.6 |
4 |
107453417 |
- |
Glis1 |
NNNGAACAGAGGGCTGTCAT |
NGG |
2 |
0.1286 |
Tier II |
| 8 |
NC_000068.7 |
2 |
169865330 |
- |
Tshz2 |
NNNGAGCAGAGGGATGTGAA |
NGG |
2 |
0.0952 |
Tier II |
| 9 |
NC_000080.6 |
14 |
70641320 |
+ |
Fgf17 |
NNNGCACAGAGGGATGGCAA |
NGG |
2 |
0.0588 |
Tier II |
| 10 |
NC_000085.6 |
19 |
39725693 |
+ |
Cyp2c68 |
NNNGAACAGAAGGAGGTCAA |
NGG |
2 |
0.05 |
Tier II |
| 11 |
NC_000085.6 |
19 |
39793895 |
+ |
Cyp2c40 |
NNNGAACAGAAGGAGGTCAA |
NGG |
2 |
0.05 |
Tier II |
| 12 |
NC_000085.6 |
19 |
39868808 |
+ |
Cyp2c69 |
NNNGAACAGAAGGAGGTCAA |
NGG |
2 |
0.05 |
Tier II |
| 13 |
NC_000072.6 |
6 |
83041143 |
+ |
Loxl3 |
NNNGAACAGAGGGAGGTCAG |
NGG |
2 |
0.0382 |
Tier II |
| 14 |
NC_000076.6 |
10 |
86395767 |
+ |
Syn3 |
NNNCAACAGAGGGATCTCAA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000072.6 |
6 |
88151817 |
- |
Gm38708 |
NNNGAATAGAGGGATGCCAA |
NGG |
2 |
0.5735 |
Tier III |
| 16 |
NC_000079.6 |
13 |
28689427 |
- |
2610307P16Rik |
NNNGGAGAGAGGGATGTCAA |
NGG |
2 |
0.3388 |
Tier III |
| 17 |
NC_000077.6 |
11 |
114490295 |
+ |
Gm39472 |
NNNGAAAAGATGGATGTCAA |
NGG |
2 |
0.2885 |
Tier III |
| 18 |
NC_000078.6 |
12 |
77759320 |
- |
Gm35440 |
NNNGAACAGAGGCAAGTCAA |
NGG |
2 |
0.2438 |
Tier III |
| 19 |
NC_000068.7 |
2 |
45246395 |
- |
Vis1 |
NNNGAACTGAGGGATGTCAC |
NGG |
2 |
0.0974 |
Tier III |
Other clones with same target sequence:
(none)
