Construct: sgRNA BRDN0001147885
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAACGTAACTTTCGCACCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTBK2 (146057)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77968
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
42777123 |
+ |
TTBK2 |
NNNCGTAACTTTCGCACCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
64166168 |
+ |
RNF180 |
NNNCTTAACTTTCGCACCTG |
NGG |
2 |
0.1615 |
Tier II |
| 3 |
NC_000017.11 |
17 |
9402783 |
+ |
STX8 |
NNNCTTAACTTTCGCAGCAG |
NGG |
2 |
0.0176 |
Tier II |
| 4 |
NC_000002.12 |
2 |
220429528 |
+ |
LOC105373893 |
NNNAGTAACTTTAGCACCAG |
NGG |
2 |
0.3239 |
Tier III |
| 5 |
NC_000020.11 |
20 |
48016075 |
+ |
LOC107985436 |
NNNCGTATCTTTCCCACCAG |
NGG |
2 |
0.1837 |
Tier III |
| 6 |
NC_000020.11 |
20 |
48016075 |
+ |
LOC107985437 |
NNNCGTATCTTTCCCACCAG |
NGG |
2 |
0.1837 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
28915162 |
+ |
Snd1 |
NNNTGTAACTTTAGCACCAG |
NGG |
2 |
0.3077 |
Tier II |
| 2 |
NC_000073.6 |
7 |
81128617 |
- |
Slc28a1 |
NNNCGTTACTTTGGCACCAG |
NGG |
2 |
0.0597 |
Tier II |
Other clones with same target sequence:
(none)
