Construct: sgRNA BRDN0001147887

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCAGGGATCCTTTACAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSKH2 (85481)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77268

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 86064349 - PSKH2 NNNAGGGATCCTTTACAGAG NGG 0 1.0 Tier I
2 NC_000001.11 1 3523026 + MEGF6 NNNAGGGAAACTTTACAGAG NGG 2 0.8048 Tier II
3 NC_000008.11 8 122808768 + ZHX2 NNNAGGAATCCATTACAGAG NGG 2 0.8 Tier II
4 NC_000020.11 20 31537774 - HM13 NNNAGGGATCCCCTACAGAG NGG 2 0.7479 Tier II
5 NC_000017.11 17 56395201 + ANKFN1 NNNAGGGATTATTTACAGAG NGG 2 0.7059 Tier II
6 NC_000017.11 17 49752414 + FAM117A NNNAGGGGTCCCTTACAGAG NGG 2 0.6947 Tier II
7 NC_000001.11 1 237645610 + RYR2 NNNAGGGGTCCTTTACAGAA NGG 2 0.6875 Tier II
8 NC_000009.12 9 28258660 + LINGO2 NNNAGAGAGCCTTTACAGAG NGG 2 0.619 Tier II
9 NC_000008.11 8 123229462 - C8orf76 NNNAGGAAGCCTTTACAGAG NGG 2 0.619 Tier II
10 NC_000008.11 8 123229462 - ZHX1-C8orf76 NNNAGGAAGCCTTTACAGAG NGG 2 0.619 Tier II
11 NC_000010.11 10 14655935 + FAM107B NNNAGGAAGCCTTTACAGAG NGG 2 0.619 Tier II
12 NC_000010.11 10 96579576 + TM9SF3 NNNAGGAATCCTTAACAGAG NGG 2 0.619 Tier II
13 NC_000015.10 15 60697467 + RORA NNNAAGGATCCTTTACAAAG NGG 2 0.6 Tier II
14 NC_000003.12 3 32137878 + GPD1L NNNTGGGATCATTTACAGAG NGG 2 0.4773 Tier II
15 NC_000022.11 22 17095919 - IL17RA NNNAGGGTTCATTTACAGAG NGG 2 0.3214 Tier II
16 NC_000019.10 19 45449252 + ERCC1 NNNCGTGATCCTTTACAGAG NGG 2 0.2353 Tier II
17 NC_000002.12 2 158245807 + CCDC148 NNNAGGGATCATTTATAGAG NGG 2 0.2308 Tier II
18 NC_000005.10 5 9790139 + TAS2R1 NNNAGGGATCCAGTACAGAG NGG 2 0.2087 Tier II
19 NC_000021.9 21 46362922 - PCNT NNNAGGGATCCTGTGCAGAG NGG 2 0.1696 Tier II
20 NC_000010.11 10 94166630 - PLCE1 NNNAGGGATCCTTTGCAGAG NAG 2 0.1685 Tier II
21 NC_000017.11 17 56068195 - ANKFN1 NNNAGGGATGCTTCACAGAG NGG 2 0.1111 Tier II
22 NC_000002.12 2 111997691 + MERTK NNNAGGGTTCCTTTACAGAG NAG 2 0.1111 Tier II
23 NC_000005.10 5 90727707 - ADGRV1 NNNAGGGATCCTTTAAAGAG NGA 2 0.0694 Tier II
24 NC_000003.12 3 138220405 + ARMC8 NNNAGGGATACTTTACAGAG NGA 2 0.0602 Tier II
25 NC_000017.11 17 17173756 - MPRIP NNNAGGGATCCCTTACAGAG NTG 2 0.0369 Tier II
26 NC_000010.11 10 14655935 + LOC105376429 NNNAGGAAGCCTTTACAGAG NGG 2 0.619 Tier III
27 NC_000020.11 20 48399887 + LOC105372643 NNNAGGGACCCTTTGCAGAG NGG 2 0.6 Tier III
28 NC_000009.12 9 79836340 + LINC01507 NNNAGGGATCCTGTACAGAA NGG 2 0.2446 Tier III
29 NC_000005.10 5 9790139 + LINC02112 NNNAGGGATCCAGTACAGAG NGG 2 0.2087 Tier III
30 NC_000005.10 5 90727707 - LOC105379077 NNNAGGGATCCTTTAAAGAG NGA 2 0.0694 Tier III
31 NC_000001.11 1 211541362 + LOC107985260 NNNAGGGATCCTTTACAAAG NGA 2 0.0481 Tier III
32 NC_000001.11 1 211541362 + LOC107985261 NNNAGGGATCCTTTACAAAG NGA 2 0.0481 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 20351880 - Zfp560 NNNACGGAACCTTTACAGAG NGG 2 0.7296 Tier I
2 NC_000077.6 11 114897715 - Cd300a NNNTGGGAGCCTTTACAGAG NGG 2 0.3939 Tier I
3 NC_000070.6 4 108853349 + Txndc12 NNNTGGAATCCTTTACAGAG NGG 2 0.6364 Tier II
4 NC_000083.6 17 8753513 + Pde10a NNNAGTGATCCCTTACAGAG NGG 2 0.6316 Tier II
5 NC_000086.7 X 99832019 + Tmem28 NNNAGGCATCATTTACAGAG NGG 2 0.5156 Tier II
6 NC_000076.6 10 120088403 + Helb NNNAGGGAGCCTTTACACAG NGG 2 0.2948 Tier II
7 NC_000076.6 10 77512527 - Fam207a NNNAGGGAACCTTTATAGAG NGG 2 0.2857 Tier II
8 NC_000085.6 19 42965503 - Hpse2 NNNATGGAACCTTTACAGAG NGG 2 0.2786 Tier II
9 NC_000067.6 1 8747281 - Sntg1 NNNAAGGATCCTTCACAGAG NGG 2 0.2476 Tier II
10 NC_000082.6 16 41693868 - Lsamp NNNATGGTTCCTTTACAGAG NGG 2 0.1286 Tier II
11 NC_000086.7 X 70470553 + Tmem185a NNNAGGGATCCTGTACAGAC NGG 2 0.1118 Tier II
12 NC_000067.6 1 13633887 + Lactb2 NNNAGAGATCCTTTACAGAG NCG 2 0.1071 Tier II
13 NC_000073.6 7 117405841 - Xylt1 NNNAGGGATCCTTCACAGAG NAG 2 0.0741 Tier II
14 NC_000074.6 8 119271032 - Cdh13 NNNAGGGATCCTTTACTGAG NAG 2 0.0346 Tier II
15 NC_000073.6 7 67024793 + Adamts17 NNNAGGGAGCCTTTACAGAG NTG 2 0.0241 Tier II
16 NC_000078.6 12 17223368 - Gm36568 NNNGGGGATTCTTTACAGAG NGG 2 0.5882 Tier III
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Other clones with same target sequence:

(none)