Construct: sgRNA BRDN0001147892
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATTGGACGAGGAGGATTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK3 (157)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77945
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
25674486 |
+ |
GRK3 |
NNNTGGACGAGGAGGATTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
11778149 |
+ |
DNAH9 |
NNNTGGAAGAAGAGGATTCG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000001.11 |
1 |
2160816 |
+ |
PRKCZ |
NNNTGGACGTGGAGGACTCG |
NGG |
2 |
0.6228 |
Tier II |
| 4 |
NC_000011.10 |
11 |
44114635 |
- |
EXT2 |
NNNTAGAGGAGGAGGATTCG |
NGG |
2 |
0.5571 |
Tier II |
| 5 |
NC_000016.10 |
16 |
88889714 |
- |
CBFA2T3 |
NNNTGGAGGAGGAGGAATCG |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000016.10 |
16 |
88889762 |
- |
CBFA2T3 |
NNNTGGAGGAGGAGGAATCG |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000016.10 |
16 |
88889841 |
- |
CBFA2T3 |
NNNTGGAGGAGGAGGAATCG |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000016.10 |
16 |
88889920 |
- |
CBFA2T3 |
NNNTGGAGGAGGAGGAATCG |
NGG |
2 |
0.3429 |
Tier II |
| 9 |
NC_000016.10 |
16 |
88890031 |
- |
CBFA2T3 |
NNNTGGAGGAGGAGGAATCG |
NGG |
2 |
0.3429 |
Tier II |
| 10 |
NC_000016.10 |
16 |
88890080 |
- |
CBFA2T3 |
NNNTGGAGGAGGAGGAATCG |
NGG |
2 |
0.3429 |
Tier II |
| 11 |
NC_000001.11 |
1 |
31188671 |
- |
NKAIN1 |
NNNAGGAAGAGGAGGATTCG |
NGG |
2 |
0.3095 |
Tier II |
| 12 |
NC_000023.11 |
X |
49059353 |
+ |
CCDC120 |
NNNTGGACGAGGAGGGATCG |
NGG |
2 |
0.1026 |
Tier II |
| 13 |
NC_000016.10 |
16 |
29860422 |
- |
CDIPT |
NNNTGGAGGAGGAGGATTGG |
NGG |
2 |
0.0804 |
Tier II |
| 14 |
NC_000011.10 |
11 |
9762640 |
+ |
SBF2-AS1 |
NNNTGGACAAGGAGGATACG |
NGG |
2 |
0.4286 |
Tier III |
| 15 |
NC_000012.12 |
12 |
32106427 |
+ |
BICD1-AS1 |
NNNGGGACGAGGAGGATGCG |
NGG |
2 |
0.2083 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
14118805 |
+ |
Psmd6 |
NNNTGGAAGAGGAGGATTCG |
NGG |
1 |
0.65 |
Tier II |
| 2 |
NC_000071.6 |
5 |
101806506 |
+ |
Cds1 |
NNNTGGAGGAGGGGGATTCG |
NGG |
2 |
0.4193 |
Tier II |
| 3 |
NC_000083.6 |
17 |
56862462 |
- |
Acsbg2 |
NNNTGGACCAGGAGGATTCG |
NTG |
2 |
0.021 |
Tier II |
| 4 |
NC_000071.6 |
5 |
24314536 |
+ |
Gm15589 |
NNNTGGACGAGGACGACTCG |
NGG |
2 |
0.3025 |
Tier III |
Other clones with same target sequence:
(none)
