Construct: sgRNA BRDN0001147898
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGGGCGTTCTATGCAGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK2 (10110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76209
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
43567959 |
+ |
SGK2 |
NNNGGCGTTCTATGCAGTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
74882695 |
+ |
FADS6 |
NNNGGCGTGCTCTGCAGTGA |
NGG |
2 |
0.1629 |
Tier I |
| 3 |
NC_000007.14 |
7 |
36450707 |
+ |
ANLN |
NNNGGCCTTCTATGCAGTGG |
NGG |
2 |
0.5257 |
Tier II |
| 4 |
NC_000008.11 |
8 |
138784727 |
- |
COL22A1 |
NNNGGCGTTGAATGCAGTGA |
NGG |
2 |
0.2917 |
Tier II |
| 5 |
NC_000020.11 |
20 |
57655459 |
+ |
PMEPA1 |
NNNGGCGTGCTATGCAGTCA |
NGG |
2 |
0.2775 |
Tier II |
| 6 |
NC_000004.12 |
4 |
525428 |
- |
PIGG |
NNNGGAGTTCTCTGCAGTGA |
NGG |
2 |
0.2444 |
Tier II |
| 7 |
NC_000011.10 |
11 |
69813247 |
+ |
FGF3 |
NNNGGCGTTCCAGGCAGTGA |
NGG |
2 |
0.1739 |
Tier II |
| 8 |
NC_000009.12 |
9 |
98135880 |
- |
CORO2A |
NNNGGGGTTCTATGCAGTGA |
NAG |
2 |
0.1296 |
Tier II |
| 9 |
NC_000006.12 |
6 |
144679984 |
+ |
UTRN |
NNNGGAGTTCTATGTAGTGA |
NGG |
2 |
0.0619 |
Tier II |
| 10 |
NC_000017.11 |
17 |
850150 |
- |
NXN |
NNNGGCGTTCTAAGCAGTGA |
NGA |
2 |
0.0481 |
Tier II |
| 11 |
NC_000011.10 |
11 |
22810056 |
- |
GAS2 |
NNNGGCGTTCTATGCAGTGA |
NCA |
2 |
0.0 |
Tier II |
| 12 |
NC_000008.11 |
8 |
82474677 |
+ |
LOC105375931 |
NNNGGAGTTCTATGAAGTGA |
NGG |
2 |
0.2063 |
Tier III |
| 13 |
NC_000007.14 |
7 |
1160789 |
- |
ZFAND2A-DT |
NNNGGCGTTCTGTGCAGTGA |
NCG |
2 |
0.0774 |
Tier III |
| 14 |
NC_000017.11 |
17 |
850150 |
- |
LOC124903894 |
NNNGGCGTTCTAAGCAGTGA |
NGA |
2 |
0.0481 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
78724739 |
- |
Slc10a7 |
NNNGGAGTTCTATGCAGTGC |
NGG |
2 |
0.211 |
Tier II |
| 2 |
NC_000083.6 |
17 |
31099138 |
- |
Abcg1 |
NNNGCCGTTCTAGGCAGTGA |
NGG |
2 |
0.205 |
Tier II |
| 3 |
NC_000079.6 |
13 |
29403661 |
+ |
Cdkal1 |
NNNGGCTTTCTCTGCAGTGA |
NGG |
2 |
0.1504 |
Tier II |
| 4 |
NC_000068.7 |
2 |
158856278 |
+ |
Dhx35 |
NNNGGCGTTCTTTCCAGTGA |
NGG |
2 |
0.1429 |
Tier II |
| 5 |
NC_000073.6 |
7 |
56478639 |
- |
Oca2 |
NNNGGTGTTCTATGCAGTGA |
NTG |
2 |
0.0362 |
Tier II |
| 6 |
NC_000068.7 |
2 |
106241633 |
+ |
Dcdc5 |
NNNGGCCTTCTATGCAGTGA |
NTG |
2 |
0.0268 |
Tier II |
| 7 |
NC_000077.6 |
11 |
35962754 |
- |
Wwc1 |
NNNGGCGCTCTATGCAGTGA |
NGC |
2 |
0.0222 |
Tier II |
| 8 |
NC_000072.6 |
6 |
139901096 |
- |
Pik3c2g |
NNNGGGGTTCTATGCTGTGA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000079.6 |
13 |
89766296 |
- |
Gm4117 |
NNNGGCCTTTTATGCAGTGA |
NGG |
2 |
0.6471 |
Tier III |
Other clones with same target sequence:
(none)
