Construct: sgRNA BRDN0001147899
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGTCCAGGATTTGTCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ARAF (369)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75660
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
47565279 |
+ |
ARAF |
NNNGTCCAGGATTTGTCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
1893233 |
+ |
MYT1L |
NNNGTCCAGGATTTATCCGA |
NGG |
2 |
0.8824 |
Tier II |
| 3 |
NC_000011.10 |
11 |
19715381 |
+ |
NAV2 |
NNNGTACAGGATTTGTCCAG |
NGG |
2 |
0.6633 |
Tier II |
| 4 |
NC_000007.14 |
7 |
87507251 |
+ |
ABCB1 |
NNNGACCAGGATTTGTCCTG |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000009.12 |
9 |
85609765 |
- |
AGTPBP1 |
NNNGTCCAGGATTTGGCCAG |
NGG |
2 |
0.2473 |
Tier II |
| 6 |
NC_000002.12 |
2 |
143733083 |
+ |
ARHGAP15 |
NNNGTCCAGGATTTCTCCAG |
NGG |
2 |
0.1948 |
Tier II |
| 7 |
NC_000020.11 |
20 |
34967869 |
+ |
MYH7B |
NNNGTCCAGGATTTGTGGGG |
NGG |
2 |
0.0078 |
Tier II |
| 8 |
NC_000002.12 |
2 |
143733083 |
+ |
ARHGAP15-AS1 |
NNNGTCCAGGATTTCTCCAG |
NGG |
2 |
0.1948 |
Tier III |
| 9 |
NC_000003.12 |
3 |
35194776 |
+ |
LOC101928135 |
NNNGTCCAGGATTTGTGCAG |
NGG |
2 |
0.042 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
158026323 |
- |
Tti1 |
NNNGTCCAGGATTTTGCCGG |
NGG |
2 |
0.0495 |
Tier II |
Other clones with same target sequence:
(none)
