Construct: sgRNA BRDN0001147900
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTTATGAGAGGTTTAACCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBCK (93627)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75564
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
106235295 |
- |
TBCK |
NNNTATGAGAGGTTTAACCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
103936513 |
- |
RELN |
NNNAATTAGAGGTTTAACCT |
NGG |
2 |
0.2721 |
Tier II |
| 3 |
NC_000002.12 |
2 |
61389836 |
- |
USP34 |
NNNTCTGAGAGTTTTAACCT |
NGG |
2 |
0.1923 |
Tier II |
| 4 |
NC_000007.14 |
7 |
79311993 |
- |
MAGI2 |
NNNTATGAGAGGTTTAGACT |
NGG |
2 |
0.095 |
Tier II |
| 5 |
NC_000005.10 |
5 |
138980919 |
+ |
SIL1 |
NNNTATGAGAGGTTCACCCT |
NGG |
2 |
0.0481 |
Tier II |
| 6 |
NC_000004.12 |
4 |
104290917 |
+ |
LOC105377350 |
NNNGATGAGAAGTTTAACCT |
NGG |
2 |
0.625 |
Tier III |
| 7 |
NC_000001.11 |
1 |
165542141 |
- |
LRRC52-AS1 |
NNNTATGAGTGGTTTAACCT |
NAG |
2 |
0.2288 |
Tier III |
| 8 |
NC_000022.11 |
22 |
20306402 |
+ |
PRODHLP |
NNNTAAGAGAGGTTTAGCCT |
NGG |
2 |
0.1529 |
Tier III |
| 9 |
NC_000005.10 |
5 |
124202209 |
+ |
LINC01170 |
NNNTATGAGAGGTTAACCCT |
NGG |
2 |
0.1022 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
132732239 |
+ |
Tbck |
NNNTATGAGAGGTTTAACCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
30216324 |
+ |
Sptbn1 |
NNNTATGAGAAGTTAAACCT |
NGG |
2 |
0.5789 |
Tier II |
| 3 |
NC_000083.6 |
17 |
70482857 |
+ |
Dlgap1 |
NNNTAAGAGAGGTTTAAACT |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000070.6 |
4 |
83827216 |
+ |
Ccdc171 |
NNNTATGAGAGGATTAATCT |
NGG |
2 |
0.4451 |
Tier II |
| 5 |
NC_000076.6 |
10 |
43494674 |
- |
Bend3 |
NNNAATGAGAGGCTTAACCT |
NGG |
2 |
0.3759 |
Tier II |
| 6 |
NC_000082.6 |
16 |
85085632 |
+ |
App |
NNNTATGATAGGTATAACCT |
NGG |
2 |
0.3302 |
Tier II |
| 7 |
NC_000080.6 |
14 |
122934037 |
+ |
Tmtc4 |
NNNTAAGAGAGGTTTACCCT |
NGG |
2 |
0.1529 |
Tier II |
| 8 |
NC_000077.6 |
11 |
32387777 |
+ |
Sh3pxd2b |
NNNTTTGAGAGGTTTAGCCT |
NGG |
2 |
0.0642 |
Tier II |
| 9 |
NC_000076.6 |
10 |
42228600 |
+ |
Foxo3 |
NNNTATGAGAAGTTTAACCT |
NTG |
2 |
0.039 |
Tier II |
| 10 |
NC_000077.6 |
11 |
76572243 |
- |
Abr |
NNNTATGAGAGGTCTAACCC |
NGG |
2 |
0.026 |
Tier II |
| 11 |
NC_000077.6 |
11 |
36543823 |
- |
Tenm2 |
NNNTCTGAGAGGTTTAACCT |
NTG |
2 |
0.0195 |
Tier II |
| 12 |
NC_000085.6 |
19 |
57814632 |
- |
Atrnl1 |
NNNTATGAGAGGTTGAACCT |
NGA |
2 |
0.0035 |
Tier II |
| 13 |
NC_000075.6 |
9 |
32208929 |
+ |
Arhgap32 |
NNNTGTGAGAGGTTTTACCT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000070.6 |
4 |
83827216 |
+ |
Gm42309 |
NNNTATGAGAGGATTAATCT |
NGG |
2 |
0.4451 |
Tier III |
| 15 |
NC_000069.6 |
3 |
122384600 |
+ |
Gm4610 |
NNNTATGTGAAGTTTAACCT |
NGG |
2 |
0.4286 |
Tier III |
| 16 |
NC_000080.6 |
14 |
55177576 |
+ |
Gm46455 |
NNNTTTGAGAGATTTAACCT |
NGG |
2 |
0.3394 |
Tier III |
| 17 |
NC_000082.6 |
16 |
86877268 |
+ |
Gm32624 |
NNNTATGACAGCTTTAACCT |
NGG |
2 |
0.2851 |
Tier III |
| 18 |
NC_000078.6 |
12 |
27171060 |
- |
Gm40872 |
NNNCATGAGAGGTTTAACGT |
NGG |
2 |
0.0809 |
Tier III |
| 19 |
NC_000086.7 |
X |
69950822 |
- |
1700111N16Rik |
NNNTATGAGAGGTTTATCCC |
NGG |
2 |
0.0121 |
Tier III |
Other clones with same target sequence:
(none)
