Construct: sgRNA BRDN0001147903

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCTTGGATTGAGTCAACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGR (2268)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75721

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 27621581 + FGR NNNTTGGATTGAGTCAACAG NGG 0 1.0 Tier I
2 NC_000012.12 12 121428148 + KDM2B NNNTTAGATTGAGTCAACAA NGG 2 0.9375 Tier II
3 NC_000005.10 5 134367002 - CDKL3 NNNTTGGATAAAGTCAACAG NGG 2 0.8571 Tier II
4 NC_000013.11 13 75766180 - LMO7 NNNTTGGATTAAGTCAACAT NGG 2 0.7 Tier II
5 NC_000004.12 4 166895962 - SPOCK3 NNNTTGAAGTGAGTCAACAG NGG 2 0.619 Tier II
6 NC_000002.12 2 63287863 + WDPCP NNNTAGGTTTGAGTCAACAG NGG 2 0.2143 Tier II
7 NC_000010.11 10 28133447 - MPP7 NNNTGGGATTGAGTCACCAG NGG 2 0.1129 Tier II
8 NC_000017.11 17 4549219 - MYBBP1A NNNTTGGGTTGAGTCATCAG NGG 2 0.0978 Tier II
9 NC_000006.12 6 105133997 - BVES NNNTTGGATTCAGTCACCAG NGG 2 0.0756 Tier II
10 NC_000017.11 17 74972171 + HID1 NNNTTGCATTGAGTCAACAG NGA 2 0.0477 Tier II
11 NC_000004.12 4 13590122 + BOD1L1 NNNTTGGATTGAGTCAACTG NGA 2 0.0374 Tier II
12 NC_000015.10 15 60520206 + RORA NNNTTGGATTAAGTCAACAG NGT 2 0.0161 Tier II
13 NC_000004.12 4 173214518 + GALNT7 NNNTTAGATTGAGTCTACAG NGG 2 0.0 Tier II
14 NC_000012.12 12 113201453 + IQCD NNNTTGGATTGAGGCCACAG NGG 2 0.0 Tier II
15 NC_000017.11 17 74972171 + HID1-AS1 NNNTTGCATTGAGTCAACAG NGA 2 0.0477 Tier III
16 NC_000018.10 18 1123060 + LOC105371953 NNNTGGGATTGAGTGAACAG NGG 2 0.032 Tier III
17 NC_000002.12 2 18287706 + LOC105373451 NNNTTGGATTTAGTTAACAG NGG 2 0.0256 Tier III
18 NC_000015.10 15 60520206 + RORA-AS1 NNNTTGGATTAAGTCAACAG NGT 2 0.0161 Tier III
19 NC_000003.12 3 111036730 - LOC151760 NNNTTGGATTGACTCCACAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 100247690 + Atf1 NNNTTTGATTGAATCAACAG NGG 2 0.6154 Tier II
2 NC_000077.6 11 97412663 - Arhgap23 NNNTGGGACTGAGTCAACAG NGG 2 0.5908 Tier II
3 NC_000067.6 1 44716754 + Gulp1 NNNTTGGATTCAGTCAACAG NGG 1 0.4286 Tier II
4 NC_000077.6 11 78420335 + Slc13a2 NNNGTTGATTGAGTCAACAG NGG 2 0.4167 Tier II
5 NC_000076.6 10 52897475 - Slc35f1 NNNTTGGATTGTGTCAACAA NGG 2 0.3125 Tier II
6 NC_000068.7 2 165189144 - Cdh22 NNNGTGGATTGAGTCAGCAG NGG 2 0.1103 Tier II
7 NC_000086.7 X 142726994 + Tmem164 NNNTTGGATTGAGCCAACAG NCG 2 0.0306 Tier II
8 NC_000079.6 13 60488716 - A530001N23Rik NNNTTGGATTGAGTCCACAG NGC 2 0.0 Tier III
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Other clones with same target sequence:

(none)