Construct: sgRNA BRDN0001147903
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTTGGATTGAGTCAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGR (2268)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75721
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
27621581 |
+ |
FGR |
NNNTTGGATTGAGTCAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
121428148 |
+ |
KDM2B |
NNNTTAGATTGAGTCAACAA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000005.10 |
5 |
134367002 |
- |
CDKL3 |
NNNTTGGATAAAGTCAACAG |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000013.11 |
13 |
75766180 |
- |
LMO7 |
NNNTTGGATTAAGTCAACAT |
NGG |
2 |
0.7 |
Tier II |
| 5 |
NC_000004.12 |
4 |
166895962 |
- |
SPOCK3 |
NNNTTGAAGTGAGTCAACAG |
NGG |
2 |
0.619 |
Tier II |
| 6 |
NC_000002.12 |
2 |
63287863 |
+ |
WDPCP |
NNNTAGGTTTGAGTCAACAG |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000010.11 |
10 |
28133447 |
- |
MPP7 |
NNNTGGGATTGAGTCACCAG |
NGG |
2 |
0.1129 |
Tier II |
| 8 |
NC_000017.11 |
17 |
4549219 |
- |
MYBBP1A |
NNNTTGGGTTGAGTCATCAG |
NGG |
2 |
0.0978 |
Tier II |
| 9 |
NC_000006.12 |
6 |
105133997 |
- |
BVES |
NNNTTGGATTCAGTCACCAG |
NGG |
2 |
0.0756 |
Tier II |
| 10 |
NC_000017.11 |
17 |
74972171 |
+ |
HID1 |
NNNTTGCATTGAGTCAACAG |
NGA |
2 |
0.0477 |
Tier II |
| 11 |
NC_000004.12 |
4 |
13590122 |
+ |
BOD1L1 |
NNNTTGGATTGAGTCAACTG |
NGA |
2 |
0.0374 |
Tier II |
| 12 |
NC_000015.10 |
15 |
60520206 |
+ |
RORA |
NNNTTGGATTAAGTCAACAG |
NGT |
2 |
0.0161 |
Tier II |
| 13 |
NC_000004.12 |
4 |
173214518 |
+ |
GALNT7 |
NNNTTAGATTGAGTCTACAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000012.12 |
12 |
113201453 |
+ |
IQCD |
NNNTTGGATTGAGGCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000017.11 |
17 |
74972171 |
+ |
HID1-AS1 |
NNNTTGCATTGAGTCAACAG |
NGA |
2 |
0.0477 |
Tier III |
| 16 |
NC_000018.10 |
18 |
1123060 |
+ |
LOC105371953 |
NNNTGGGATTGAGTGAACAG |
NGG |
2 |
0.032 |
Tier III |
| 17 |
NC_000002.12 |
2 |
18287706 |
+ |
LOC105373451 |
NNNTTGGATTTAGTTAACAG |
NGG |
2 |
0.0256 |
Tier III |
| 18 |
NC_000015.10 |
15 |
60520206 |
+ |
RORA-AS1 |
NNNTTGGATTAAGTCAACAG |
NGT |
2 |
0.0161 |
Tier III |
| 19 |
NC_000003.12 |
3 |
111036730 |
- |
LOC151760 |
NNNTTGGATTGACTCCACAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
100247690 |
+ |
Atf1 |
NNNTTTGATTGAATCAACAG |
NGG |
2 |
0.6154 |
Tier II |
| 2 |
NC_000077.6 |
11 |
97412663 |
- |
Arhgap23 |
NNNTGGGACTGAGTCAACAG |
NGG |
2 |
0.5908 |
Tier II |
| 3 |
NC_000067.6 |
1 |
44716754 |
+ |
Gulp1 |
NNNTTGGATTCAGTCAACAG |
NGG |
1 |
0.4286 |
Tier II |
| 4 |
NC_000077.6 |
11 |
78420335 |
+ |
Slc13a2 |
NNNGTTGATTGAGTCAACAG |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000076.6 |
10 |
52897475 |
- |
Slc35f1 |
NNNTTGGATTGTGTCAACAA |
NGG |
2 |
0.3125 |
Tier II |
| 6 |
NC_000068.7 |
2 |
165189144 |
- |
Cdh22 |
NNNGTGGATTGAGTCAGCAG |
NGG |
2 |
0.1103 |
Tier II |
| 7 |
NC_000086.7 |
X |
142726994 |
+ |
Tmem164 |
NNNTTGGATTGAGCCAACAG |
NCG |
2 |
0.0306 |
Tier II |
| 8 |
NC_000079.6 |
13 |
60488716 |
- |
A530001N23Rik |
NNNTTGGATTGAGTCCACAG |
NGC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
