GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001147906

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: AAGTTGATGACAGCAACACT

Original Target:

Taxon:: Homo sapiens (human)
Gene:: DDR2 (4921)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77974

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000001.11	1	162767258	+	DDR2	NNNTTGATGACAGCAACACT	NGG	0	1.0	Tier I
2	NC_000013.11	13	25373953	-	ATP8A2	NNNTTCAAGACAGCAACACT	NGG	2	0.5455	Tier II
3	NC_000017.11	17	33656849	-	ASIC2	NNNTTGATTTCAGCAACACT	NGG	2	0.4706	Tier II
4	NC_000010.11	10	11845617	+	PROSER2	NNNTAGAGGACAGCAACACT	NGG	2	0.3667	Tier II
5	NC_000012.12	12	128409457	+	TMEM132C	NNNAGGATGACAGCAACACT	NGG	2	0.3048	Tier II
6	NC_000006.12	6	83144012	+	DOP1A	NNNTTGTTAACAGCAACACT	NGG	2	0.2812	Tier II
7	NC_000008.11	8	414781	+	FBXO25	NNNTTTATGACAGCAACGCT	NGG	2	0.2667	Tier II
8	NC_000001.11	1	220763209	-	MTARC2	NNNTTGATGGCAGCAACAAT	NGG	2	0.2381	Tier II
9	NC_000013.11	13	36310039	+	SPART	NNNCTGATGACAGAAACACT	NGG	2	0.2265	Tier II
10	NC_000011.10	11	48046446	-	PTPRJ	NNNTGGATGACAGCAACCCT	NGG	2	0.1219	Tier II
11	NC_000008.11	8	51548915	-	PXDNL	NNNTTGAAGACAGCAAGACT	NGG	2	0.0471	Tier II
12	NC_000001.11	1	95225616	+	TLCD4-RWDD3	NNNTTGGTGACAGCAAGACT	NGG	2	0.0415	Tier II
13	NC_000008.11	8	14685929	+	SGCZ	NNNTTCATGACAGCAAGACT	NGG	2	0.0401	Tier II
14	NC_000006.12	6	158635318	-	TMEM181	NNNCTGATGACAGCAACACT	NTG	2	0.0252	Tier II
15	NC_000016.10	16	20778157	+	ACSM3	NNNTTGCTGACAGCAACACT	NGT	2	0.0071	Tier II
16	NC_000002.12	2	36762323	+	VIT	NNNTTGATGACAGTATCACT	NGG	2	0.0	Tier II
17	NC_000003.12	3	105003129	+	LOC105374023	NNNTGGATGACAGCAACACT	NGG	1	0.64	Tier III
18	NC_000013.11	13	94828191	+	LOC101927284	NNNCTGTTGACAGCAACACT	NGG	2	0.2831	Tier III
19	NC_000008.11	8	11365618	+	TDH	NNNTTGATGACAGCAATGCT	NGG	2	0.1867	Tier III
20	NC_000009.12	9	134541479	+	LOC100506532	NNNATGATGACAGCAGCACT	NGG	2	0.0916	Tier III
21	NC_000015.10	15	98331353	-	LINC02351	NNNTTGATGCCAGCAACCCT	NGG	2	0.0635	Tier III
22	NC_000001.11	1	95225616	+	RWDD3-DT	NNNTTGGTGACAGCAAGACT	NGG	2	0.0415	Tier III
23	NC_000015.10	15	91644858	+	LOC105370973	NNNTAGATGACAGGAACACT	NGG	2	0.0	Tier III
24	NC_000002.12	2	36762323	+	LOC124905990	NNNTTGATGACAGTATCACT	NGG	2	0.0	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000067.6	1	169994687	-	Ddr2	NNNTTGATGATAGCAACACT	NGG	1	0.3077	Tier I
2	NC_000071.6	5	57942169	-	Pcdh7	NNNGTGATGACAGCAACACC	NGG	2	0.0568	Tier I
3	NC_000081.6	15	64183355	-	Asap1	NNNCTGATGTCAGCAACACT	NGG	2	0.5709	Tier II
4	NC_000076.6	10	121393525	-	Gns	NNNTTAATCACAGCAACACT	NGG	2	0.5385	Tier II
5	NC_000067.6	1	87519485	-	Ngef	NNNTTGATCACAGCAACACT	NGG	1	0.5385	Tier II
6	NC_000080.6	14	63700814	+	Xkr6	NNNTTGAGAACAGCAACACT	NGG	2	0.4714	Tier II
7	NC_000076.6	10	24892064	-	Med23	NNNTTGAAGACAGAAACACT	NGG	2	0.28	Tier II
8	NC_000082.6	16	24790792	-	Lpp	NNNTTGAAGACTGCAACACT	NGG	2	0.2667	Tier II
9	NC_000075.6	9	63713886	+	Smad3	NNNTTGATGTGAGCAACACT	NGG	2	0.2206	Tier II
10	NC_000076.6	10	56149866	+	Tbc1d32	NNNTTGATGGCAGAAACACT	NGG	2	0.1944	Tier II
11	NC_000085.6	19	47311191	+	Sh3pxd2a	NNNTGGATGACAGCCACACT	NGG	2	0.1745	Tier II
12	NC_000085.6	19	5763628	-	Scyl1	NNNGTGATGACAGCCACACT	NGG	2	0.1705	Tier II
13	NC_000084.6	18	12512265	-	Lama3	NNNTGGATGACAGCTACACT	NGG	2	0.128	Tier II
14	NC_000080.6	14	56171098	+	Gzmn	NNNTTGATGCCTGCAACACT	NGG	2	0.1111	Tier II
15	NC_000078.6	12	11393317	+	Vsnl1	NNNTTGATGGCAGCAACAGT	NGG	2	0.0694	Tier II
16	NC_000070.6	4	58799034	-	Ecpas	NNNTTGATGACATCAACACC	NGG	2	0.0273	Tier II
17	NC_000072.6	6	30678723	-	Cep41	NNNGTGATGACAGCATCACT	NGG	2	0.0	Tier II
18	NC_000072.6	6	30678723	-	Gm13782	NNNGTGATGACAGCATCACT	NGG	2	0.0	Tier III
19	NC_000082.6	16	9002083	+	Gm30444	NNNTTGATGCCAGGAACACT	NGG	2	0.0	Tier III

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Other clones with same target sequence:

(none)