Construct: sgRNA BRDN0001147908

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCACCCAGTTCCAAACACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ULK1 (8408)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75738

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 131914473 + ULK1 NNNCCCAGTTCCAAACACCT NGG 0 1.0 Tier I
2 NC_000012.12 12 104507958 - CHST11 NNNCCCAATTCCATACACCT NGG 2 0.5333 Tier II
3 NC_000004.12 4 17627492 - MED28 NNNCCCAGGACCAAACACCT NGG 2 0.5306 Tier II
4 NC_000007.14 7 148153569 - CNTNAP2 NNNCCCAGGTCCGAACACCT NGG 2 0.4037 Tier II
5 NC_000015.10 15 69395549 + PAQR5 NNNCACAGTTCTAAACACCT NGG 2 0.3077 Tier II
6 NC_000019.10 19 38354677 + CATSPERG NNNCCCAGATCCAACCACCT NGG 2 0.2532 Tier II
7 NC_000005.10 5 20024320 - CDH18 NNNCCCAGTCCCAGACACCT NGG 2 0.2489 Tier II
8 NC_000003.12 3 64141690 + PRICKLE2 NNNCCCAGTTCTAAACACAT NGG 2 0.2308 Tier II
9 NC_000019.10 19 34664980 - SCGB2B2 NNNCCCTGTGCCAAACACCT NGG 2 0.2188 Tier II
10 NC_000012.12 12 612366 + NINJ2 NNNCCCAATTCCACACACCT NGG 2 0.2143 Tier II
11 NC_000015.10 15 84607955 + ZSCAN2 NNNCCCAGTTCCACACACCT NGG 1 0.2143 Tier II
12 NC_000020.11 20 38489159 - RALGAPB NNNCCCAATTCCAATCACCT NGG 2 0.2 Tier II
13 NC_000007.14 7 89247143 + ZNF804B NNNCCTAGTTCCACACACCT NGG 2 0.199 Tier II
14 NC_000001.11 1 93498909 + FNBP1L NNNCCCAGTTTCAAACATCT NGG 2 0.1978 Tier II
15 NC_000003.12 3 45950291 + FYCO1 NNNCCAAGTTCCCAACACCT NGG 2 0.1955 Tier II
16 NC_000007.14 7 95100754 - PPP1R9A NNNCCCAGTTCCAAAAGCCT NGG 2 0.1765 Tier II
17 NC_000009.12 9 133117561 - RALGDS NNNCCCAGTTCCGCACACCT NGG 2 0.1398 Tier II
18 NC_000011.10 11 119687249 + NECTIN1 NNNCCCAGTTCCCAGCACCT NGG 2 0.1368 Tier II
19 NC_000006.12 6 38990807 + DNAH8 NNNCCCAGTTCCAAGCCCCT NGG 2 0.1147 Tier II
20 NC_000004.12 4 101298101 + PPP3CA NNNCTCAGTTCCAAAGACCT NGG 2 0.0979 Tier II
21 NC_000020.11 20 61850440 + CDH4 NNNCCCAGTTCCATACGCCT NGG 2 0.0941 Tier II
22 NC_000009.12 9 36843876 + PAX5 NNNCCCAGTTCCAAGCACCC NGG 2 0.0591 Tier II
23 NC_000015.10 15 68334417 - ITGA11 NNNCCCAGTTCCAACCAGCT NGG 2 0.0364 Tier II
24 NC_000018.10 18 37259839 + CELF4 NNNCCCAGTTCCACACACCC NGG 2 0.0195 Tier II
25 NC_000011.10 11 134490567 + B3GAT1-DT NNNCCCATTTCCAAACACCA NGG 2 0.3516 Tier III
26 NC_000019.10 19 34664980 - SCGB1B2P NNNCCCTGTGCCAAACACCT NGG 2 0.2188 Tier III
27 NC_000019.10 19 34664980 - ZNF807P NNNCCCTGTGCCAAACACCT NGG 2 0.2188 Tier III
28 NC_000002.12 2 2914760 - LINC01250 NNNCCCAGTTCCAGACACTT NGG 2 0.2154 Tier III
29 NC_000015.10 15 84607955 + LOC105370947 NNNCCCAGTTCCACACACCT NGG 1 0.2143 Tier III
30 NC_000007.14 7 95100754 - PPP1R9A-AS1 NNNCCCAGTTCCAAAAGCCT NGG 2 0.1765 Tier III
31 NC_000001.11 1 27661342 - LINC02574 NNNCCCAGCTCCAAACACCC NGG 2 0.0839 Tier III
32 NC_000016.10 16 18388949 - PKD1P5 NNNCCCAGTTCCAGACCCCT NGG 2 0.0824 Tier III
33 NC_000016.10 16 18388949 - PKD1P5-LOC105376752 NNNCCCAGTTCCAGACCCCT NGG 2 0.0824 Tier III
34 NC_000013.11 13 19314341 - ANKRD26P3 NNNCCCAGTTGCAACCACCT NGG 2 0.0682 Tier III
35 NC_000003.12 3 129613690 + LOC124909431 NNNCCCAGTTCTAAACACCT NGC 2 0.012 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 95681657 - Adamtsl4 NNNCCCAGTTCCAATTACCT NGG 2 0.0615 Tier I
2 NC_000070.6 4 100989325 - Cachd1 NNNCCAAATTCCAAACACCT NGG 2 0.9286 Tier II
3 NC_000074.6 8 17116805 - Csmd1 NNNCCCAGCTACAAACACCT NGG 2 0.6923 Tier II
4 NC_000086.7 X 12127111 + Bcor NNNCCCAGCTCCAAGCACCT NGG 2 0.6 Tier II
5 NC_000072.6 6 38880702 - Tbxas1 NNNCCCTGCTCCAAACACCT NGG 2 0.4038 Tier II
6 NC_000073.6 7 63468010 - Otud7a NNNCCCAGTTCAAAACACCA NGG 2 0.4018 Tier II
7 NC_000082.6 16 28557219 - Fgf12 NNNCCCAGTTCAAAACACTT NGG 2 0.3297 Tier II
8 NC_000081.6 15 47626444 - Csmd3 NNNCCCAGTGCCATACACCT NGG 2 0.2667 Tier II
9 NC_000074.6 8 119286754 + Cdh13 NNNCCCCGTTCCAAACACCA NGG 2 0.2461 Tier II
10 NC_000077.6 11 35480127 + Slit3 NNNCCCAGGTTCAAACACCT NGG 2 0.1905 Tier II
11 NC_000071.6 5 108623997 + Gak NNNCCCATTTCCTAACACCT NGG 2 0.1875 Tier II
12 NC_000075.6 9 29269595 - Ntm NNNCCCAGTTCCAATCATCT NGG 2 0.1286 Tier II
13 NC_000067.6 1 75183931 - Atg9a NNNCCCACTTCCAAACAGCT NGG 2 0.0821 Tier II
14 NC_000075.6 9 21287830 + Kri1 NNNCCCAGTTCCCCACACCT NGG 2 0.0451 Tier II
15 NC_000073.6 7 130874584 + Plekha1 NNNCCCAGTTCCAAACAACT NGA 2 0.0374 Tier II
16 NC_000077.6 11 72318675 + Fbxo39 NNNCCCAGTCCCAAACACCT NGA 2 0.037 Tier II
17 NC_000076.6 10 62749708 - Ccar1 NNNCCCAGTTCCCAACACCC NGG 2 0.0191 Tier II
18 NC_000067.6 1 120091116 + Gm41938 NNNCTCAGTTCCAAACACAT NGG 2 0.2727 Tier III
19 NC_000072.6 6 127189104 - 9330179D12Rik NNNCCCAGTTCCAGACACCT NGT 2 0.0075 Tier III
Download CSV

Other clones with same target sequence:

(none)