Construct: sgRNA BRDN0001147912
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGTTGATCACGCCATCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAPSS2 (9060)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76317
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
87727284 |
- |
PAPSS2 |
NNNGTTGATCACGCCATCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
62814469 |
+ |
EGR2 |
NNNGTTGATCATGCCATCTG |
NGG |
1 |
0.5385 |
Tier I |
| 3 |
NC_000007.14 |
7 |
103624339 |
+ |
RELN |
NNNGCTGATCACGCCACCTG |
NGG |
2 |
0.7059 |
Tier II |
| 4 |
NC_000008.11 |
8 |
133022683 |
+ |
TG |
NNNGTTGATCACCCCATCTA |
NGG |
2 |
0.3947 |
Tier II |
| 5 |
NC_000017.11 |
17 |
9892037 |
- |
GLP2R |
NNNGTTGATCACCCCATTTG |
NGG |
2 |
0.2707 |
Tier II |
| 6 |
NC_000004.12 |
4 |
55082917 |
- |
KDR |
NNNGTTGATCACTCCATCTG |
NTG |
2 |
0.0117 |
Tier II |
| 7 |
NC_000010.11 |
10 |
118243350 |
+ |
LINC02944 |
NNNTTTGATCAAGCCATCTG |
NGG |
2 |
0.2597 |
Tier III |
| 8 |
NC_000008.11 |
8 |
118654707 |
- |
SAMD12-AS1 |
NNNGTTGAGCACTCCATCTG |
NGG |
2 |
0.1857 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
67539745 |
- |
Egr2 |
NNNGTTGATCATGCCATCTG |
NGG |
1 |
0.5385 |
Tier I |
| 2 |
NC_000078.6 |
12 |
98511942 |
+ |
Kcnk10 |
NNNGATGATCACGCCATCTT |
NGG |
2 |
0.35 |
Tier II |
| 3 |
NC_000074.6 |
8 |
118301174 |
+ |
Cdh13 |
NNNGTTGATCAAGCCATCTG |
NGA |
2 |
0.0496 |
Tier II |
| 4 |
NC_000068.7 |
2 |
116877249 |
+ |
Gm13990 |
NNNGTTAATCAAGCCATCTG |
NGG |
2 |
0.7143 |
Tier III |
| 5 |
NC_000074.6 |
8 |
118301174 |
+ |
Gm51555 |
NNNGTTGATCAAGCCATCTG |
NGA |
2 |
0.0496 |
Tier III |
Other clones with same target sequence:
(none)
