Construct: sgRNA BRDN0001147917
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAGTCGAGAGAAACAAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK1 (27102)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77047
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
6038592 |
- |
EIF2AK1 |
NNNGTCGAGAGAAACAAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
16554283 |
- |
RETREG1 |
NNNGTCCAGAGAAACAAGCG |
NGG |
1 |
0.6875 |
Tier II |
| 3 |
NC_000007.14 |
7 |
147363936 |
- |
CNTNAP2 |
NNNGTTGACAGAAACAAGCG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000008.11 |
8 |
143417884 |
+ |
MAFA-AS1 |
NNNGCCGAGAGAAACAAGCT |
NGG |
2 |
0.7 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
103221482 |
+ |
Lrriq1 |
NNNGTTCAGAGAAACAAGCG |
NGG |
2 |
0.6384 |
Tier II |
| 2 |
NC_000070.6 |
4 |
120724887 |
+ |
Kcnq4 |
NNNGTCAAGAGAAGCAAGCG |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000073.6 |
7 |
122507581 |
+ |
Prkcb |
NNNGTAGAGAGAAACAAGCG |
NTG |
2 |
0.0362 |
Tier II |
| 4 |
NC_000075.6 |
9 |
118423497 |
- |
Platr11 |
NNNTTCGAGAGAAACAAGTG |
NGG |
2 |
0.1678 |
Tier III |
Other clones with same target sequence:
(none)
