Construct: sgRNA BRDN0001147919

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAGGTCCGAGATCCGCACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK2 (156)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75684

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 67282306 - GRK2 NNNGGTCCGAGATCCGCACG NGG 0 1.0 Tier I
2 NC_000015.10 15 89088931 + LOC124903573 NNNGGTCCGGGATCCGCACC NGG 2 0.2381 Tier I
3 NC_000015.10 15 89088931 + ABHD2 NNNGGTCCGGGATCCGCACC NGG 2 0.2381 Tier II
4 NC_000010.11 10 117163133 + MIR3663HG NNNGGTCCGGGATCCGCACC NGG 2 0.2381 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 48500433 - Sspo NNNGGTTCAAGATCCGCACG NGG 2 0.5223 Tier I
2 NC_000079.6 13 55453179 - Grk6 NNNGGTCCGAGATCCGAATG NGG 2 0.2154 Tier I
3 NC_000079.6 13 55453179 - Mir6943 NNNGGTCCGAGATCCGAATG NGG 2 0.2154 Tier III
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Other clones with same target sequence:

(none)