Construct: sgRNA BRDN0001147920
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCCGCCTTAAAACTCCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKA (3706)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76855
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
41501635 |
- |
ITPKA |
NNNCGCCTTAAAACTCCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
91843668 |
- |
TC2N |
NNNCGTCCTAAAACTCCCTG |
NGG |
2 |
0.9286 |
Tier II |
| 3 |
NC_000018.10 |
18 |
6252918 |
+ |
L3MBTL4 |
NNNAGCCTTAAAACCCCCTG |
NGG |
2 |
0.2297 |
Tier II |
| 4 |
NC_000022.11 |
22 |
46917856 |
- |
TBC1D22A |
NNNCGCCATAAACCTCCCTG |
NGG |
2 |
0.1684 |
Tier II |
| 5 |
NC_000014.9 |
14 |
91206131 |
+ |
DGLUCY |
NNNGGCCTTAAATCTCCCTG |
NGG |
2 |
0.15 |
Tier II |
| 6 |
NC_000008.11 |
8 |
55368298 |
+ |
XKR4 |
NNNCTCGTTAAAACTCCCTG |
NGG |
2 |
0.1412 |
Tier II |
| 7 |
NC_000019.10 |
19 |
49336601 |
- |
CD37 |
NNNCTCCTTAAACCTCCCTG |
NGG |
2 |
0.0632 |
Tier II |
| 8 |
NC_000019.10 |
19 |
49336601 |
- |
SLC6A16 |
NNNCTCCTTAAACCTCCCTG |
NGG |
2 |
0.0632 |
Tier II |
| 9 |
NC_000014.9 |
14 |
91843668 |
- |
LOC124903362 |
NNNCGTCCTAAAACTCCCTG |
NGG |
2 |
0.9286 |
Tier III |
| 10 |
NC_000008.11 |
8 |
95613595 |
+ |
CFAP418-AS1 |
NNNCACCTTAAAACTCTCTG |
NGG |
2 |
0.4044 |
Tier III |
| 11 |
NC_000001.11 |
1 |
187093281 |
- |
LINC01036 |
NNNCGCTTTAATACTCCCTG |
NGG |
2 |
0.2708 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
58313028 |
+ |
Acvr1c |
NNNCGCCTTTAAACTCCCTG |
NGG |
1 |
0.8824 |
Tier II |
| 2 |
NC_000084.6 |
18 |
88991931 |
+ |
Rttn |
NNNCACCTCAAAACTCCCTG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000069.6 |
3 |
106556998 |
- |
Dram2 |
NNNCACCTTTAAACTCCCTG |
NGG |
2 |
0.7647 |
Tier II |
| 4 |
NC_000073.6 |
7 |
60321583 |
+ |
Snrpn |
NNNGGCCTTTAAACTCCCTG |
NGG |
2 |
0.4412 |
Tier II |
| 5 |
NC_000071.6 |
5 |
127463634 |
- |
Tmem132c |
NNNCACCTTAAAACTCCCAG |
NGG |
2 |
0.2476 |
Tier II |
| 6 |
NC_000082.6 |
16 |
33575029 |
+ |
Slc12a8 |
NNNCGCCTTAAAAGTCCCTG |
NGG |
1 |
0.0 |
Tier II |
| 7 |
NC_000083.6 |
17 |
14463222 |
- |
Gm38551 |
NNNCCCCTTAAAGCTCCCTG |
NGG |
2 |
0.5124 |
Tier III |
| 8 |
NC_000073.6 |
7 |
60321583 |
+ |
Snhg14 |
NNNGGCCTTTAAACTCCCTG |
NGG |
2 |
0.4412 |
Tier III |
| 9 |
NC_000074.6 |
8 |
63762826 |
+ |
Gm35424 |
NNNGACCTTAAAACTCCCTG |
NGG |
2 |
0.4333 |
Tier III |
| 10 |
NC_000068.7 |
2 |
95440608 |
+ |
Gm13794 |
NNNCTCCTTAAAACTCCTTG |
NGG |
2 |
0.1929 |
Tier III |
Other clones with same target sequence:
(none)
