Construct: sgRNA BRDN0001147921

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTGAAGTAGTAAATAGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA6 (27330)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75536

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 84119934 - RPS6KA6 NNNGAAGTAGTAAATAGGAG NGG 0 1.0 Tier I
2 NC_000003.12 3 119317935 + ARHGAP31 NNNGAAGTAGTGAATAGGAA NGG 2 0.6771 Tier II
3 NC_000003.12 3 10524644 - ATP2B2 NNNGAAGTAATAAATAGAAG NGG 2 0.6462 Tier II
4 NC_000004.12 4 93397017 + GRID2 NNNGAATTAGTAAATAGGAG NGG 1 0.5714 Tier II
5 NC_000014.9 14 88263956 + KCNK10 NNNGAGGAAGTAAATAGGAG NGG 2 0.5714 Tier II
6 NC_000001.11 1 14184279 - KAZN NNNGAAGGAGAAAATAGGAG NGG 2 0.55 Tier II
7 NC_000005.10 5 149850190 - PPARGC1B NNNGAAGAAGCAAATAGGAG NGG 2 0.5333 Tier II
8 NC_000015.10 15 32843763 + FMN1 NNNGAAGTAGTAAATAGCAA NGG 2 0.4464 Tier II
9 NC_000004.12 4 174817126 - GLRA3 NNNGAAGTTGTAAATAGAAG NGG 2 0.4154 Tier II
10 NC_000015.10 15 61856015 - VPS13C NNNGCATTAGTAAATAGGAG NGG 2 0.2857 Tier II
11 NC_000001.11 1 246125904 + SMYD3 NNNGTAGTAGTAAAAAGGAG NGG 2 0.2105 Tier II
12 NC_000017.11 17 49969075 - DLX4 NNNGAAGTGGTAAATGGGAG NGG 2 0.1282 Tier II
13 NC_000002.12 2 45989388 + PRKCE NNNTAAGTAGTAAACAGGAG NGG 2 0.0992 Tier II
14 NC_000003.12 3 120944312 + STXBP5L NNNGAAGTAGGAAATGGGAG NGG 2 0.0769 Tier II
15 NC_000002.12 2 178017065 - PDE11A NNNGAAGCAGTAAATAGGAG NGA 2 0.0694 Tier II
16 NC_000012.12 12 106440715 - POLR3B NNNAAAGTAGTAAAGAGGAG NGG 2 0.045 Tier II
17 NC_000007.14 7 132534145 + PLXNA4 NNNGTAGTAGTAAAGAGGAG NGG 2 0.0182 Tier II
18 NC_000014.9 14 35154498 + PRORP NNNGAAATAGTAAATAGGAG NGT 2 0.0161 Tier II
19 NC_000003.12 3 54901606 - CACNA2D3 NNNGAAGCAGTAAATAGGAG NGT 2 0.0161 Tier II
20 NC_000007.14 7 103443050 - SLC26A5 NNNGAACTAGTAAATAGGAG NGT 2 0.0111 Tier II
21 NC_000010.11 10 104701328 + SORCS3 NNNGAAGTAGTACATAGGAG NGT 2 0.0034 Tier II
22 NC_000015.10 15 30622839 - ARHGAP11B-DT NNNGAAGTAGAAAATAGGAA NGG 2 0.7031 Tier III
23 NC_000015.10 15 32612233 - ARHGAP11A-DT NNNGAAGTAGAAAATAGGAA NGG 2 0.7031 Tier III
24 NC_000003.12 3 119317935 + ARHGAP31-AS1 NNNGAAGTAGTGAATAGGAA NGG 2 0.6771 Tier III
25 NC_000021.9 21 34746891 + LINC01426 NNNGAAGGAGTAGATAGGAG NGG 2 0.4783 Tier III
26 NC_000015.10 15 61856015 - LOC124903501 NNNGCATTAGTAAATAGGAG NGG 2 0.2857 Tier III
27 NC_000008.11 8 69359141 - LOC124901958 NNNGAAGTAGTAAATAAGAG NAG 2 0.242 Tier III
28 NC_000011.10 11 116342997 + LOC107987166 NNNGAAGTAGGAAGTAGGAG NGG 2 0.1867 Tier III
29 NC_000008.11 8 111158800 + LINC01609 NNNGAAGTAGTAAATGAGAG NGG 2 0.1795 Tier III
30 NC_000023.11 X 131400668 - LOC102723546 NNNAAAGTAGTAAATGGGAG NGG 2 0.1731 Tier III
31 NC_000023.11 X 131400668 - LOC107985705 NNNAAAGTAGTAAATGGGAG NGG 2 0.1731 Tier III
32 NC_000012.12 12 18768560 - LOC102724227 NNNCAAGTAGTAAACAGGAG NGG 2 0.1444 Tier III
33 NC_000023.11 X 128323345 + LOC107985698 NNNGAAGGAGTAAATGGGAG NGG 2 0.141 Tier III
34 NC_000007.14 7 91506738 + LINC02932 NNNAAAGTAGTAAATAGGAG NTG 2 0.0351 Tier III
35 NC_000014.9 14 35154498 + PRORP-PSMA6 NNNGAAATAGTAAATAGGAG NGT 2 0.0161 Tier III
36 NC_000001.11 1 207224803 - LOC107985251 NNNGAAGTAGTAAATGGGAG NGC 2 0.0043 Tier III
37 NC_000012.12 12 81979545 - LINC02426 NNNGAAATAGTAAATTGGAG NGG 2 0.0 Tier III
38 NC_000004.12 4 131117694 - LOC105377422 NNNGAAGTAGTAAATAGGAG NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 38815677 - Olfr922 NNNGAAGTAGTACATAGGAG NGT 2 0.0034 Tier I
2 NC_000077.6 11 74045092 + Olfr139 NNNGAAGTAGTACATAGGAG NGT 2 0.0034 Tier I
3 NC_000067.6 1 154872421 + Cacna1e NNNGAAGTAGTAAAAAGGAA NGG 2 0.5428 Tier II
4 NC_000070.6 4 113802319 + Skint5 NNNGAAGTAGAAAATAGAAG NGG 2 0.5192 Tier II
5 NC_000071.6 5 65805312 - N4bp2 NNNGGAGTGGTAAATAGGAG NGG 2 0.48 Tier II
6 NC_000079.6 13 63849519 + Ercc6l2 NNNGAAGTAGTAAAAAGGAT NGG 2 0.4053 Tier II
7 NC_000071.6 5 105435502 + Lrrc8b NNNGAAGTTGTAGATAGGAG NGG 2 0.3913 Tier II
8 NC_000086.7 X 164238588 + Bmx NNNATAGTAGTAAATAGGAG NGG 2 0.3273 Tier II
9 NC_000078.6 12 111890977 + Ppp1r13b NNNGAGGTAGGAAATAGGAG NGG 2 0.2857 Tier II
10 NC_000069.6 3 16195585 - Ythdf3 NNNGAAGTAGGAAATAGAAG NGG 2 0.2769 Tier II
11 NC_000077.6 11 115473001 + Slc16a5 NNNGAAGTAGGAAATAGAAG NGG 2 0.2769 Tier II
12 NC_000082.6 16 94610882 + Dyrk1a NNNGAAGTAATAAACAGGAG NGG 2 0.2545 Tier II
13 NC_000080.6 14 59665994 + Atp8a2 NNNGAAGGAGTTAATAGGAG NGG 2 0.2444 Tier II
14 NC_000073.6 7 92857715 - Ddias NNNGAAGTCGTTAATAGGAG NGG 2 0.1905 Tier II
15 NC_000069.6 3 24501548 + Naaladl2 NNNGAAGTGGTAAATAGGAG NAG 2 0.1728 Tier II
16 NC_000076.6 10 100458030 + Tmtc3 NNNGAAGTAGTCAAAAGGAG NGG 2 0.1524 Tier II
17 NC_000068.7 2 121362394 - Ckmt1 NNNGAAGTAGTAAACAGGAC NGG 2 0.1169 Tier II
18 NC_000073.6 7 44349153 - Shank1 NNNGAAGTAGTAAAAGGGAG NGG 2 0.1113 Tier II
19 NC_000080.6 14 98099517 - Dach1 NNNGAAATAGTAAATAGGAG NGA 2 0.0694 Tier II
20 NC_000069.6 3 105494453 - Kcnd3 NNNGAAGTAGAAAAGAGGAG NGG 2 0.0375 Tier II
21 NC_000070.6 4 96782495 + Gm12695 NNNGAAGTAGAAAAGAGGAG NGG 2 0.0375 Tier II
22 NC_000068.7 2 73297646 + Cir1 NNNGAAGTAGGAAATAGGAG NTG 2 0.0156 Tier II
23 NC_000082.6 16 36185318 - Cstdc4 NNNGAAGTAGTAGATAGGAG NGC 2 0.0145 Tier II
24 NC_000070.6 4 132681278 - Eya3 NNNGAAGTAGTAAATTGGAG NGT 2 0.0 Tier II
25 NC_000073.6 7 61598015 - B230209E15Rik NNNGAAGCAGTAAATAGGAG NTG 2 0.039 Tier III
26 NC_000070.6 4 22940835 + Gm30886 NNNTAAGTAGTAAATCGGAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)