Construct: sgRNA BRDN0001147924

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTTCCCTTTCACACTAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEC (7006)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77023

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 48146372 + TEC NNNTCCCTTTCACACTAACT NGG 0 1.0 Tier I
2 NC_000006.12 6 138181239 - ARFGEF3 NNNTCCCCTACACACTAACT NGG 2 0.8571 Tier II
3 NC_000011.10 11 108565379 + EXPH5 NNNTCTATTTCACACTAACT NGG 2 0.6964 Tier II
4 NC_000005.10 5 111477964 + CAMK4 NNNTCCCTTTCACACTAAAT NGG 1 0.4286 Tier II
5 NC_000015.10 15 45469475 - SLC30A4-AS1 NNNTTCCTTTCACACTAGCT NGG 2 0.2545 Tier II
6 NC_000003.12 3 120410097 + FSTL1 NNNTCCCTTTCTCACCAACT NGG 2 0.2222 Tier II
7 NC_000002.12 2 148984166 - KIF5C NNNTCACTTTCACACTGACT NGG 2 0.1639 Tier II
8 NC_000007.14 7 154173480 + DPP6 NNNTCCCTTTCACCCTAACA NGG 2 0.1205 Tier II
9 NC_000012.12 12 132506547 - FBRSL1 NNNTACCTTTCACAGTAACT NGG 2 0.0286 Tier II
10 NC_000012.12 12 51334957 + CELA1 NNNTCCCATTCACACTAACT NGT 2 0.0129 Tier II
11 NC_000001.11 1 213457521 + RPS6KC1 NNNTCCCTTTCACACTCACT NGT 2 0.0028 Tier II
12 NC_000023.11 X 65797215 + ETF1P3 NNNTCACTTCCACACTAACT NGG 2 0.4952 Tier III
13 NC_000003.12 3 120410097 + BTNL12P NNNTCCCTTTCTCACCAACT NGG 2 0.2222 Tier III
14 NC_000007.14 7 32624082 + DPY19L1P1 NNNTCCCTTTCCCACCAACT NGG 2 0.1754 Tier III
15 NC_000005.10 5 25206175 - LINC02211 NNNTCCCTTTCACCCTAAAT NGG 2 0.0918 Tier III
16 NC_000005.10 5 118534923 + LINC02208 NNNTCCCTTTCACACTGACT NGA 2 0.0123 Tier III
17 NC_000007.14 7 150838304 - LOC105375567 NNNTCCCTTTCACATTAACT NGA 2 0.0046 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 127473719 - Prr14 NNNTCCCTTTCACACTTACC NGG 2 0.0121 Tier I
2 NC_000073.6 7 102001799 - Numa1 NNNTCTCTTTCACACAAACT NGG 2 0.8442 Tier II
3 NC_000077.6 11 113556960 + Slc39a11 NNNACCCCTTCACACTAACT NGG 2 0.4762 Tier II
4 NC_000067.6 1 54689957 - Ankrd44 NNNTCCCCTTCACACTAAAT NGG 2 0.4286 Tier II
5 NC_000083.6 17 32016504 - Hsf2bp NNNTCCCTTTCAAACAAACT NGG 2 0.3497 Tier II
6 NC_000082.6 16 34037197 - Kalrn NNNTCCCTTTCACACCAGCT NGG 2 0.2667 Tier II
7 NC_000070.6 4 141492861 + Spen NNNTCACTTTCACACTAACT NAG 2 0.2407 Tier II
8 NC_000085.6 19 46783899 - Cnnm2 NNNTCCCTTTCACACTAGCA NGG 2 0.225 Tier II
9 NC_000071.6 5 114788687 - Ankrd13a NNNTCCCTTCCACACGAACT NGG 2 0.1846 Tier II
10 NC_000072.6 6 32349123 + Plxna4 NNNTCCCTTGCTCACTAACT NGG 2 0.1667 Tier II
11 NC_000067.6 1 138501195 + Nek7 NNNTTCCTTTCACACTAACT NAG 2 0.165 Tier II
12 NC_000074.6 8 93529350 - Ces5a NNNTCCCTTACACACTCACT NGG 2 0.1513 Tier II
13 NC_000067.6 1 179226037 + Smyd3 NNNGCCCTTTCACACTGACT NGG 2 0.1103 Tier II
14 NC_000084.6 18 78003990 - Epg5 NNNTCCCTTTCACGATAACT NGG 2 0.1037 Tier II
15 NC_000085.6 19 31681132 + Prkg1 NNNTCCTTTTCACATTAACT NGG 2 0.0542 Tier II
16 NC_000069.6 3 89438836 - Pbxip1 NNNTCCCTTTCACAATACCT NGG 2 0.0423 Tier II
17 NC_000072.6 6 122282836 + Klrg1 NNNTCCCTTTCACACTCAGT NGG 2 0.0221 Tier II
18 NC_000077.6 11 52933993 - Fstl4 NNNTCCCTTTCACACTAACT NTC 2 0.0 Tier II
19 NC_000067.6 1 54689957 - 4930444A19Rik NNNTCCCCTTCACACTAAAT NGG 2 0.4286 Tier III
20 NC_000075.6 9 91351309 + Gm40545 NNNTCCCTTTCACTCTCACT NGG 2 0.0941 Tier III
21 NC_000075.6 9 91351309 + Gm40546 NNNTCCCTTTCACTCTCACT NGG 2 0.0941 Tier III
22 NC_000068.7 2 118998275 + Gm14089 NNNTCCCTTTCACACTCACT NCG 2 0.0189 Tier III
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Other clones with same target sequence:

(none)