Construct: sgRNA BRDN0001147929

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGAGGTGGCTTTAGGTTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80254

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 30659061 + USPL1 NNNAGGTGACTTTAGGTATT NGG 2 0.4286 Tier I
2 NC_000006.12 6 150843287 + PLEKHG1 NNNACATGGCTTTAGGTTTT NGG 2 0.7857 Tier II
3 NC_000012.12 12 86206461 + MGAT4C NNNAGGTGGTATTAGGTTTT NGG 2 0.7059 Tier II
4 NC_000004.12 4 88622412 + HERC3 NNNGGGTGGTTTTAGGTTTT NGG 2 0.5882 Tier II
5 NC_000006.12 6 110854090 + AMD1 NNNAGATGGCTTTTGGTTTT NGG 2 0.5333 Tier II
6 NC_000014.9 14 90052605 + LOC124903406 NNNAGGTGACTTCAGGTTTT NGG 2 0.5075 Tier II
7 NC_000004.12 4 176235614 + ASB5 NNNAGGTGGCTTTTAGTTTT NGG 2 0.502 Tier II
8 NC_000005.10 5 127548372 + PRRC1 NNNACGTGTCTTTAGGTTTT NGG 2 0.419 Tier II
9 NC_000005.10 5 43676519 + NNT NNNAGGTGGATTTGGGTTTT NGG 2 0.4044 Tier II
10 NC_000003.12 3 2984695 + CNTN4 NNNCGATGGCTTTAGGTTTT NGG 2 0.3529 Tier II
11 NC_000011.10 11 66281042 - CNIH2 NNNAGGTGGCTTCAGGTGTT NGG 2 0.2632 Tier II
12 NC_000020.11 20 19502585 - SLC24A3 NNNAGGTGGTTTTAGGTTTT NAG 2 0.244 Tier II
13 NC_000009.12 9 94444277 + MFSD14B NNNAGGTGGCTTCAGGTTGT NGG 2 0.1974 Tier II
14 NC_000016.10 16 1588186 + IFT140 NNNAGGTGGCTTTGGGTTTT NAG 2 0.121 Tier II
15 NC_000006.12 6 108317697 + AFG1L NNNAGGTGGCTTTTGGTTTT NGA 2 0.037 Tier II
16 NC_000003.12 3 28379866 + ZCWPW2 NNNAGGTGGCTTTCTGTTTT NGG 2 0.0306 Tier II
17 NC_000001.11 1 48774182 - BEND5 NNNAGGTGGCTTTATGTTTG NGG 2 0.0252 Tier II
18 NC_000001.11 1 48774182 - AGBL4 NNNAGGTGGCTTTATGTTTG NGG 2 0.0252 Tier II
19 NC_000007.14 7 77544628 + PTPN12 NNNAGGTGGCTGTAGGTTTT NTG 2 0.0195 Tier II
20 NC_000023.11 X 36011534 + CFAP47 NNNAGGTGGCTTTGGGTTTT NTG 2 0.0182 Tier II
21 NC_000011.10 11 118312385 - CD3E NNNAGGTGGCTTTAGGGTTT NCG 2 0.0126 Tier II
22 NC_000005.10 5 22625597 + CDH12 NNNAGCTGGCTTTAGCTTTT NGG 2 0.0 Tier II
23 NC_000012.12 12 99558791 - ANKS1B NNNAGGAGGCTTTAGCTTTT NGG 2 0.0 Tier II
24 NC_000005.10 5 64285775 - RNF180 NNNAGGTGGCTTTAGCATTT NGG 2 0.0 Tier II
25 NC_000005.10 5 131857430 + MEIKIN NNNAGGTGGCTTTAGCTTTT NGG 1 0.0 Tier II
26 NC_000004.12 4 88502442 + HERC5 NNNAGGTGGCTTTAGTTTTT NTG 2 0.0 Tier II
27 NC_000009.12 9 16034716 + CCDC171 NNNAGGTGGCTTTAGTTTTT NGG 1 0.0 Tier II
28 NC_000004.12 4 13862532 + LINC01182 NNNAGGTGGTTTTTGGTTTT NGG 2 0.502 Tier III
29 NC_000004.12 4 13862532 + LOC107986182 NNNAGGTGGTTTTTGGTTTT NGG 2 0.502 Tier III
30 NC_000015.10 15 45800167 + LOC105370802 NNNAGTTTGCTTTAGGTTTT NGG 2 0.4167 Tier III
31 NC_000002.12 2 60380117 - MIR4432HG NNNAGGTGGCATTTGGTTTT NGG 2 0.4 Tier III
32 NC_000016.10 16 1588186 + LOC105371046 NNNAGGTGGCTTTGGGTTTT NAG 2 0.121 Tier III
33 NC_000021.9 21 30395111 - LOC105372772 NNNAGGTGGATTTAGGTTTT NTG 2 0.0338 Tier III
34 NC_000015.10 15 65797229 - LOC105370866 NNNAGGCGGCTTTAGCTTTT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 142312490 + Slco1a5 NNNAGATGGTTTTAGGTTTT NGG 2 0.9412 Tier II
2 NC_000081.6 15 97017933 + Slc38a4 NNNAGCTGGTTTTAGGTTTT NGG 2 0.6417 Tier II
3 NC_000069.6 3 87851442 - Sh2d2a NNNAGGAGGCTTTAGGCTTT NGG 2 0.6176 Tier II
4 NC_000076.6 10 78346704 - Agpat3 NNNAGGTAGCTTTGGGTTTT NGG 2 0.4667 Tier II
5 NC_000082.6 16 36544393 + Casr NNNAGATGGCTTTAGGTCTT NGG 2 0.4286 Tier II
6 NC_000076.6 10 75424014 + Upb1 NNNAGGTGACTTTAGGTTTA NGG 2 0.3616 Tier II
7 NC_000084.6 18 66102783 + Ccbe1 NNNAGGTGTCTTTAGGTATT NGG 2 0.3556 Tier II
8 NC_000076.6 10 45443161 + Lin28b NNNAGGTTGCTTTAGGATTT NGG 2 0.3333 Tier II
9 NC_000079.6 13 76441725 + Mctp1 NNNATGTGGCCTTAGGTTTT NGG 2 0.2 Tier II
10 NC_000086.7 X 13168018 + Usp9x NNNAGGTGGCCTTACGTTTT NGG 2 0.1818 Tier II
11 NC_000067.6 1 162863224 + Fmo1 NNNAGGTTGCTTTAGGTTTT NAG 2 0.162 Tier II
12 NC_000070.6 4 104523780 - Dab1 NNNACGTGGCTTTAGGTTTG NGG 2 0.1387 Tier II
13 NC_000075.6 9 105063918 + Mrpl3 NNNAGATGGCTTTAGGGTTT NGG 2 0.1176 Tier II
14 NC_000073.6 7 134436983 + D7Ertd443e NNNAGGTGGCTTTACGTCTT NGG 2 0.1169 Tier II
15 NC_000075.6 9 118660251 + Itga9 NNNTGGTGGCTTTAGGTTTG NGG 2 0.1123 Tier II
16 NC_000077.6 11 119954129 + Baiap2 NNNAGCTGGCTTTAGGTTTC NGG 2 0.062 Tier II
17 NC_000068.7 2 70225525 - Myo3b NNNAGGTGACTTTAGGTTTC NGG 2 0.0584 Tier II
18 NC_000069.6 3 119408890 - Dpyd NNNAGGTGGCTTTAGGATTT NGA 2 0.037 Tier II
19 NC_000085.6 19 25617826 + Dmrt3 NNNAGGTGGGTTTAGGTTTT NTG 2 0.0152 Tier II
20 NC_000082.6 16 88041745 - Grik1 NNNAGGTGGCTTTAGGTTGT NGC 2 0.0056 Tier II
21 NC_000080.6 14 49722322 - Armh4 NNNAGGTGGCTTTATGTTTT NGT 2 0.0023 Tier II
22 NC_000072.6 6 129012388 + Clec2f NNNAGGTGGCTTTCGCTTTT NGG 2 0.0 Tier II
23 NC_000071.6 5 125784493 - Tmem132b NNNAGGTGGGTTTAGCTTTT NGG 2 0.0 Tier II
24 NC_000074.6 8 10732152 - Gm30666 NNNAGGTGGGTTTAGGTTTT NGG 1 0.3889 Tier III
25 NC_000077.6 11 113105223 + 2610035D17Rik NNNCGGTGGCTTTTGGTTTT NGG 2 0.1882 Tier III
26 NC_000070.6 4 30804186 - 4930556G01Rik NNNAGTTGGCTTTAGGTTTT NAG 2 0.1728 Tier III
27 NC_000078.6 12 80075899 - Gm36660 NNNAGGTGGCTTTAGGTTAC NGG 2 0.026 Tier III
28 NC_000080.6 14 49722322 - Gm36146 NNNAGGTGGCTTTATGTTTT NGT 2 0.0023 Tier III
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Other clones with same target sequence:

(none)