Construct: sgRNA BRDN0001147931

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATTCCCGAGGAGATCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K2 (5605)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77603

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 4102397 - MAP2K2 NNNTTCCCGAGGAGATCCTG NGG 0 1.0 Tier I
2 NC_000014.9 14 24414864 + NYNRIN NNNTTTCGGAGGAGATCCTG NGG 2 0.5969 Tier I
3 NC_000011.10 11 824351 + PNPLA2 NNNTTCCCGAGGACATCCGG NGG 2 0.1071 Tier I
4 NC_000019.10 19 13965461 - RFX1 NNNTCCCCGAGGAGATGCTG NGG 2 0.0588 Tier I
5 NC_000010.11 10 72088811 - SPOCK2 NNNTCCCCGGGGAGATCCTG NGG 2 0.5556 Tier II
6 NC_000009.12 9 128170211 - CIZ1 NNNTTCCCTAAGAGATCCTG NGG 2 0.5333 Tier II
7 NC_000016.10 16 85270203 + GSE1 NNNTTCCTGAGCAGATCCTG NGG 2 0.4632 Tier II
8 NC_000017.11 17 3734440 - ITGAE NNNTTCCCGAGGACACCCTG NGG 2 0.2857 Tier II
9 NC_000023.11 X 18600665 - CDKL5 NNNTTCCCGAGGAGAGCCTC NGG 2 0.1484 Tier II
10 NC_000014.9 14 37254143 + MIPOL1 NNNTTCCTGAGGAGATGCTG NGG 2 0.0515 Tier II
11 NC_000020.11 20 20040111 - CRNKL1 NNNTTCCCTAGGAGATCCTG NGA 2 0.037 Tier II
12 NC_000002.12 2 219032275 - CFAP65 NNNTTCCCCAGGAGATGCTG NGG 2 0.0317 Tier II
13 NC_000020.11 20 47055327 + EYA2 NNNTTCCCGAGGAGATCCTC NGC 2 0.0095 Tier II
14 NC_000007.14 7 129127022 + LOC407835 NNNTTCCCGAGGACATCCTG NGG 1 0.4286 Tier III
15 NC_000003.12 3 5339406 - LOC124906205 NNNTTCCCCAGGAGATTCTG NGG 2 0.2513 Tier III
16 NC_000005.10 5 172778558 + LOC105377730 NNNTTCCCTAGGAGATCCTC NGG 2 0.2286 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 82749210 - Hk2 NNNTTCCCGAGGACATCATG NGG 2 0.2308 Tier I
2 NC_000072.6 6 142565926 - Kcnj8 NNNTTGCCGAGGAGATCCAG NGG 2 0.1429 Tier I
3 NC_000075.6 9 106029987 - Col6a4 NNNTTCCCGAGGAGAGCCTG NGC 2 0.0077 Tier I
4 NC_000070.6 4 17876307 + Mmp16 NNNTCCCAGAGGAGATCCTG NGG 2 0.65 Tier II
5 NC_000071.6 5 121776175 + Atxn2 NNNTTCCCAAGGAGATCTTG NGG 2 0.4133 Tier II
6 NC_000075.6 9 14770625 + 1700012B09Rik NNNGGCCCGAGGAGATCCTG NGG 2 0.4 Tier II
7 NC_000075.6 9 108086083 - Apeh NNNTTCCCAGGGAGATCCTG NGG 2 0.3571 Tier II
8 NC_000076.6 10 61841669 - Col13a1 NNNTCCCCGAGGAGATCCAG NGG 2 0.2857 Tier II
9 NC_000071.6 5 74490278 + Scfd2 NNNATCCCCAGGAGATCCTG NGG 2 0.2564 Tier II
10 NC_000073.6 7 122096929 + Ndufab1 NNNTTCCCTAGGACATCCTG NGG 2 0.2286 Tier II
11 NC_000077.6 11 33993076 - Kcnip1 NNNGTCCCGAGGTGATCCTG NGG 2 0.1875 Tier II
12 NC_000077.6 11 101060025 + Atp6v0a1 NNNTTCCCCAGGAGCTCCTG NGG 2 0.1469 Tier II
13 NC_000068.7 2 21560371 - Gpr158 NNNATCCCGAGGTGATCCTG NGG 2 0.1429 Tier II
14 NC_000071.6 5 98423717 + Cfap299 NNNATCCCGAGGTGATCCTG NGG 2 0.1429 Tier II
15 NC_000075.6 9 76967581 - Tinag NNNATCCCGAGGTGATCCTG NGG 2 0.1429 Tier II
16 NC_000076.6 10 4818017 + Esr1 NNNATCCCGAGGTGATCCTG NGG 2 0.1429 Tier II
17 NC_000068.7 2 36234859 + Ptgs1 NNNATCCCGAGGAGCTCCTG NGG 2 0.1299 Tier II
18 NC_000073.6 7 99172425 + Dgat2 NNNTACCCGAGGAGATCCTG NAG 2 0.1296 Tier II
19 NC_000073.6 7 74371339 - Slco3a1 NNNTTCCCAAGGAGATGCTG NGG 2 0.0378 Tier II
20 NC_000075.6 9 110622752 - Nradd NNNTTCCAGAGGAGATCCTG NTG 2 0.0253 Tier II
21 NC_000077.6 11 63978801 + Cox10 NNNTTCCCCAGGAGATCCTG NTG 2 0.021 Tier II
22 NC_000079.6 13 20116138 - Elmo1 NNNTTCCCGAGGAGATCCTT NGC 2 0.0156 Tier II
23 NC_000086.7 X 72930619 + Nsdhl NNNTTCCCAAGGAGATCCTG NGC 2 0.0143 Tier II
24 NC_000080.6 14 33367788 - Arhgap22 NNNTTCCCAAGGAGATCCTG NGT 2 0.0104 Tier II
25 NC_000075.6 9 22832151 - Bbs9 NNNTTCCCGAGGAGATGCTG NTG 2 0.0023 Tier II
26 NC_000079.6 13 78319274 + Gm41007 NNNTTCCTGAGGAGATCCTG NAG 2 0.2269 Tier III
27 NC_000077.6 11 33993076 - 4930469K13Rik NNNGTCCCGAGGTGATCCTG NGG 2 0.1875 Tier III
28 NC_000076.6 10 66415401 - Gm40677 NNNATCCCGAGGTGATCCTG NGG 2 0.1429 Tier III
29 NC_000086.7 X 156030026 - Gm15155 NNNATCCCGAGGTGATCCTG NGG 2 0.1429 Tier III
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Other clones with same target sequence:

(none)