Construct: sgRNA BRDN0001147934
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTGATGGCTACCCGCGGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- AK1 (203)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000009.12 | 9 | 127871858 | - | AK1 | NNNTGATGGCTACCCGCGGG | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000012.12 | 12 | 52618222 | + | KRT73 | NNNTGATGGATACCCCCGGG | NGG | 2 | 0.0 | Tier I |
| 3 | NC_000009.12 | 9 | 127871858 | - | ST6GALNAC4-ST6GALNAC6-AK1 | NNNTGATGGCTACCCGCGGG | NGG | 0 | 1.0 | Tier III |
| 4 | NC_000008.11 | 8 | 116829197 | + | LOC112268030 | NNNTGATGGCTACCCGAGGC | NGG | 2 | 0.2 | Tier III |
| 5 | NC_000007.14 | 7 | 17298457 | + | LOC101927609 | NNNAGATGGCTACCGGCGGG | NGG | 2 | 0.0238 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000068.7 | 2 | 35463708 | - | Ggta1 | NNNTGATGGCTGGCCGCGGG | NGG | 2 | 0.0985 | Tier II |
| 2 | NC_000081.6 | 15 | 80287927 | - | Cacna1i | NNNTGATGGGTACGCGCGGG | NGG | 2 | 0.0 | Tier II |
| 3 | NC_000072.6 | 6 | 128469550 | + | Gm10069 | NNNAGATGGCTACCCGCGGG | NGG | 1 | 0.4762 | Tier III |
| 4 | NC_000081.6 | 15 | 80287927 | - | Gm31319 | NNNTGATGGGTACGCGCGGG | NGG | 2 | 0.0 | Tier III |