Construct: sgRNA BRDN0001147939

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGCTGGAGAGACTAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLK2 (11011)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75617

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 62578514 + TLK2 NNNGCTGGAGAGACTAGAAA NGG 0 1.0 Tier I
2 NC_000014.9 14 50758113 - NIN NNNGCTGGAAAGACTAGAAA NGG 1 0.9333 Tier I
3 NC_000001.11 1 225519285 - ENAH NNNGCTGGAGAGACTGGAAC NGG 2 0.0437 Tier I
4 NC_000017.11 17 34038649 - ASIC2 NNNGCTGGAGAGGCTAGAAA NGG 1 0.6522 Tier II
5 NC_000010.11 10 37819630 - ZNF248 NNNGCTGGAAAGGCTAGAAA NGG 2 0.6087 Tier II
6 NC_000006.12 6 42974271 + PEX6 NNNGGTGGAGAGACTAGAAA NGG 1 0.6 Tier II
7 NC_000003.12 3 130580763 - COL6A6 NNNGTTGGAGAAACTAGAAA NGG 2 0.5939 Tier II
8 NC_000010.11 10 46594589 - SYT15 NNNGCTGGAGAGACAAGAAA NGG 1 0.5789 Tier II
9 NC_000010.11 10 47747233 + ANXA8 NNNGCTGGAGAGACAAGAAA NGG 1 0.5789 Tier II
10 NC_000010.11 10 47747233 + SYT15B NNNGCTGGAGAGACAAGAAA NGG 1 0.5789 Tier II
11 NC_000012.12 12 8945334 + M6PR NNNGCTGAAGACACTAGAAA NGG 2 0.5294 Tier II
12 NC_000009.12 9 19291886 + DENND4C NNNGAAGGAGAGACTAGAAA NGG 2 0.4952 Tier II
13 NC_000002.12 2 213941954 - SPAG16 NNNGCTGGAGAGATAAGAAA NGG 2 0.4246 Tier II
14 NC_000001.11 1 154177837 + TPM3 NNNGCTGAAGAGACTAGGAA NGG 2 0.4 Tier II
15 NC_000003.12 3 122982649 - SEMA5B NNNGCTGCAGGGACTAGAAA NGG 2 0.4 Tier II
16 NC_000003.12 3 60139457 + FHIT NNNGCTGGAGAGGCAAGAAA NGG 2 0.3776 Tier II
17 NC_000018.10 18 45351849 + SLC14A2 NNNGCTGGAGAGGCAAGAAA NGG 2 0.3776 Tier II
18 NC_000011.10 11 57380887 + PRG3 NNNGCTGGAGAGTCTAGAAA NGG 1 0.3 Tier II
19 NC_000020.11 20 20593445 - RALGAPA2 NNNGCTGAAGAGACTAGAAA NAG 2 0.2593 Tier II
20 NC_000001.11 1 211100309 - KCNH1 NNNGTTGGAGAGACTAGGAA NGG 2 0.2545 Tier II
21 NC_000022.11 22 28546994 + TTC28 NNNGATGGAGTGACTAGAAA NGG 2 0.1758 Tier II
22 NC_000003.12 3 135174489 - EPHB1 NNNGCTGCAGAGACCAGAAA NGG 2 0.1678 Tier II
23 NC_000003.12 3 15586903 + HACL1 NNNGCTGGTGAGACTATAAA NGG 2 0.15 Tier II
24 NC_000003.12 3 97338046 + EPHA6 NNNGTTGGAGAGACTAGAAC NGG 2 0.1446 Tier II
25 NC_000022.11 22 23232005 - BCR NNNGCTGGAGAGACAAGACA NGG 2 0.1198 Tier II
26 NC_000007.14 7 84141285 - SEMA3A NNNGCTGGAGAGACTAGTCA NGG 2 0.1034 Tier II
27 NC_000019.10 19 48271374 + ZNF114 NNNGCTGGAGAGCCTAGGAA NGG 2 0.0842 Tier II
28 NC_000014.9 14 33526356 - NPAS3 NNNGCTGGAGAGCCTATAAA NGG 2 0.0526 Tier II
29 NC_000023.11 X 11204709 - ARHGAP6 NNNGCTGGAGAGACTGCAAA NGG 2 0.0452 Tier II
30 NC_000011.10 11 121513517 + SORL1 NNNGCTGGAGAGACTAAAAA NTG 2 0.0364 Tier II
31 NC_000005.10 5 39143411 - FYB1 NNNGCGGGAGAGACGAGAAA NGG 2 0.0286 Tier II
32 NC_000023.11 X 24995349 + POLA1 NNNGCTGGAGGGACTAGAAA NTG 2 0.0253 Tier II
33 NC_000012.12 12 109511670 + UBE3B NNNGCTGGAGAGAATAGAAA NGA 2 0.0243 Tier II
34 NC_000011.10 11 82981338 + RAB30 NNNGCTGGAGAGACAAGAAA NTG 2 0.0226 Tier II
35 NC_000001.11 1 21255633 + ECE1 NNNGCTGGAGAGACTGGAAA NGC 2 0.0043 Tier II
36 NC_000001.11 1 75244854 - SLC44A5 NNNGATGGAGAGACTTGAAA NGG 2 0.0 Tier II
37 NC_000010.11 10 71582072 + CDH23 NNNGCTGGAGAGACTCTAAA NGG 2 0.0 Tier II
38 NC_000011.10 11 6617955 + TPP1 NNNGCTGGAGAGACTTGAAA NGC 2 0.0 Tier II
39 NC_000001.11 1 176570228 - PAPPA2 NNNGCTGGAGAGACTTGGAA NGG 2 0.0 Tier II
40 NC_000001.11 1 180425087 - ACBD6 NNNGCTGGAGAGAGGAGAAA NGG 2 0.0 Tier II
41 NC_000005.10 5 147836795 - SPINK1 NNNGCTGGAGAGAGTAGAAA NGA 2 0.0 Tier II
42 NC_000006.12 6 44287293 + TCTE1 NNNGCTGGAGAGAGTAGAAA NGG 1 0.0 Tier II
43 NC_000003.12 3 47081346 + SETD2 NNNGCTGGGGAGACTTGAAA NGG 2 0.0 Tier II
44 NC_000002.12 2 74282281 + SLC4A5 NNNGCTGTAGAGAGTAGAAA NGG 2 0.0 Tier II
45 NC_000017.11 17 34038649 - TLK2P1 NNNGCTGGAGAGGCTAGAAA NGG 1 0.6522 Tier III
46 NC_000017.11 17 34038649 - LOC107987247 NNNGCTGGAGAGGCTAGAAA NGG 1 0.6522 Tier III
47 NC_000014.9 14 48693717 - LOC105378178 NNNGCTACAGAGACTAGAAA NGG 2 0.6154 Tier III
48 NC_000010.11 10 37819630 - TLK2P2 NNNGCTGGAAAGGCTAGAAA NGG 2 0.6087 Tier III
49 NC_000010.11 10 46594589 - SYT15-AS1 NNNGCTGGAGAGACAAGAAA NGG 1 0.5789 Tier III
50 NC_000010.11 10 47747233 + LOC102724593 NNNGCTGGAGAGACAAGAAA NGG 1 0.5789 Tier III
51 NC_000005.10 5 173657369 - LINC01863 NNNGCTGGAAAGAATAGAAA NGG 2 0.3267 Tier III
52 NC_000010.11 10 25675397 + LINC00836 NNNGCTGGAGAGACTAAAAA NAG 2 0.242 Tier III
53 NC_000001.11 1 20396444 - LINC01141 NNNGCTTGAGAGACTAGGAA NGG 2 0.2286 Tier III
54 NC_000002.12 2 19715844 - LOC105373461 NNNGCTGGAGAAACTGGAAA NGG 2 0.1795 Tier III
55 NC_000022.11 22 28546994 + LOC101929594 NNNGATGGAGTGACTAGAAA NGG 2 0.1758 Tier III
56 NC_000003.12 3 97338046 + LOC101929278 NNNGTTGGAGAGACTAGAAC NGG 2 0.1446 Tier III
57 NC_000019.10 19 48271374 + ZNF114-AS1 NNNGCTGGAGAGCCTAGGAA NGG 2 0.0842 Tier III
58 NC_000001.11 1 168401313 + LOC100505918 NNNGCTGGAGAGTCCAGAAA NGG 2 0.0818 Tier III
59 NC_000001.11 1 168401313 + LOC125312414 NNNGCTGGAGAGTCCAGAAA NGG 2 0.0818 Tier III
60 NC_000014.9 14 33526356 - LOC124903299 NNNGCTGGAGAGCCTATAAA NGG 2 0.0526 Tier III
61 NC_000006.12 6 43891056 - LINC01512 NNNGCTGTAGAGACTAGAAA NGA 2 0.0434 Tier III
62 NC_000006.12 6 43891056 - LOC105375070 NNNGCTGTAGAGACTAGAAA NGA 2 0.0434 Tier III
63 NC_000008.11 8 118839135 + LOC105375723 NNNGCTGGAGAGACTAGAGA NGA 2 0.026 Tier III
64 NC_000020.11 20 40639937 + LOC102724968 NNNGCTGGACAGAGTAGAAA NGG 2 0.0 Tier III
65 NC_000001.11 1 180425087 - LOC105371637 NNNGCTGGAGAGAGGAGAAA NGG 2 0.0 Tier III
66 NC_000014.9 14 49934759 - LOC100506446 NNNGTTGGAGAGACTTGAAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 20330000 - Ecd NNNCCAGGAGAGACTAGAAA NGG 2 0.4588 Tier I
2 NC_000071.6 5 65131722 + Klhl5 NNNGCTGGAGAGACCAGAAG NGG 2 0.2086 Tier I
3 NC_000068.7 2 22901847 + Pdss1 NNNGCTGGAGAGACTTGAAA NGT 2 0.0 Tier I
4 NC_000080.6 14 33552515 + Frmpd2 NNNGCTGGAGGGACTAAAAA NGG 2 0.6067 Tier II
5 NC_000080.6 14 10506468 + Fhit NNNGATGGAGAAACTAGAAA NGG 2 0.5333 Tier II
6 NC_000080.6 14 31048229 - Pbrm1 NNNCCTGAAGAGACTAGAAA NGG 2 0.5294 Tier II
7 NC_000075.6 9 50720850 - Dixdc1 NNNGCTGGGGAGACTAGAAG NGG 2 0.5098 Tier II
8 NC_000076.6 10 61574687 - Lrrc20 NNNCCTGGAGAGACTAGAAG NGG 2 0.4048 Tier II
9 NC_000086.7 X 72007268 - Cnga2 NNNGGTGGTGAGACTAGAAA NGG 2 0.36 Tier II
10 NC_000075.6 9 123459810 + Lars2 NNNCCTGGAGGGACTAGAAA NGG 2 0.3441 Tier II
11 NC_000069.6 3 13691504 + Ralyl NNNGATGGAGACACTAGAAA NGG 2 0.3025 Tier II
12 NC_000080.6 14 12042106 + Ptprg NNNTCTGGAGAGGCTAGAAA NGG 2 0.2372 Tier II
13 NC_000068.7 2 126149194 - Fam227b NNNGCTGGAGAGACTACAAA NGG 1 0.2353 Tier II
14 NC_000083.6 17 78709259 + Strn NNNGCTTGACAGACTAGAAA NGG 2 0.2286 Tier II
15 NC_000067.6 1 181990122 - Enah NNNTCTGCAGAGACTAGAAA NGG 2 0.2238 Tier II
16 NC_000070.6 4 129216665 - Yars NNNTCTGGAGAGACAAGAAA NGG 2 0.2105 Tier II
17 NC_000080.6 14 123926946 - Itgbl1 NNNGCTGGAGAGAAAAGAAA NGG 2 0.2026 Tier II
18 NC_000075.6 9 68964381 - Rora NNNTCTGGAGAGACTAGATA NGG 2 0.1958 Tier II
19 NC_000067.6 1 84634569 + Dner NNNGCTGGAGACAATAGAAA NGG 2 0.1853 Tier II
20 NC_000067.6 1 129487431 - Thsd7b NNNGATGGAGAGACTATAAA NGG 2 0.1429 Tier II
21 NC_000072.6 6 34066929 - Lrguk NNNGCTGGAGAGGCTAGACA NGG 2 0.1349 Tier II
22 NC_000070.6 4 150362126 + Rere NNNCCTGGAGAGACTATAAA NGG 2 0.1324 Tier II
23 NC_000069.6 3 88327844 - Glmp NNNGCTGGAGAGACAAGCAA NGG 2 0.1103 Tier II
24 NC_000068.7 2 106863703 - Mpped2 NNNGCTGGAGAGACTAGGAA NAG 2 0.1037 Tier II
25 NC_000072.6 6 77076855 - Ctnna2 NNNTCTGGAGAGACCAGAAA NGG 2 0.0992 Tier II
26 NC_000082.6 16 7022018 + Rbfox1 NNNTCTGGAGAGACGAGAAA NGG 2 0.0182 Tier II
27 NC_000075.6 9 83595599 + Sh3bgrl2 NNNGCTGGAGAGACTAGAGA NTG 2 0.0146 Tier II
28 NC_000077.6 11 86622980 - Vmp1 NNNTCTGGAGAGACTAGAAA NTG 2 0.0142 Tier II
29 NC_000081.6 15 91754341 - Lrrk2 NNNGCTGGAGAGAATCGAAA NGG 2 0.0 Tier II
30 NC_000067.6 1 155605917 + Acbd6 NNNGCTGGAGAGAGGAGAAA NGG 2 0.0 Tier II
31 NC_000084.6 18 62367317 - Htr4 NNNGCTGGAGAGAGTAGACA NGG 2 0.0 Tier II
32 NC_000071.6 5 106508485 + Gm28050 NNNACTGGAGAAACTAGAAA NGG 2 0.84 Tier III
33 NC_000074.6 8 99015289 - Gm15679 NNNGTTGGAAAGACTAGAAA NGG 2 0.5939 Tier III
34 NC_000079.6 13 80985625 + 9330111N05Rik NNNGATGGGGAGACTAGAAA NGG 2 0.381 Tier III
35 NC_000075.6 9 32594749 + Gm31871 NNNGGTGGAGAGACTAGAAT NGG 2 0.36 Tier III
36 NC_000080.6 14 61068404 + Gm41168 NNNGCTGGAGAAAATAGAAA NGG 2 0.3267 Tier III
37 NC_000076.6 10 17022028 + Gm20125 NNNGAGGGAGAGACTAGAAA NGG 2 0.3265 Tier III
38 NC_000068.7 2 178636916 - Gm40031 NNNGCTAGAGAGCCTAGAAA NGG 2 0.2105 Tier III
39 NC_000071.6 5 45284266 + D5Ertd615e NNNGCAGGAGAGACTGGAAA NGG 2 0.1667 Tier III
40 NC_000068.7 2 92819726 + Gm13791 NNNGCTGGAGAGCCAAGAAA NGG 2 0.1219 Tier III
41 NC_000068.7 2 92819726 + Gm39880 NNNGCTGGAGAGCCAAGAAA NGG 2 0.1219 Tier III
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Other clones with same target sequence:

(none)