Construct: sgRNA BRDN0001147943

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGAGCTCTCAACAGTTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUCY2F (2986)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77254

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 109398678 + GUCY2F NNNAGCTCTCAACAGTTCAG NGG 0 1.0 Tier I
2 NC_000010.11 10 128103983 - MKI67 NNNAGCTCTCAGCAGTTGAG NGG 2 0.0963 Tier I
3 NC_000011.10 11 119716366 + NECTIN1 NNNAGCTCACAACAGTCCAG NGG 2 0.6555 Tier II
4 NC_000007.14 7 158958558 + DYNC2I1 NNNAGCTCACAATAGTTCAG NGG 2 0.65 Tier II
5 NC_000002.12 2 48560139 - STON1 NNNAGGTCTTAACAGTTCAG NGG 2 0.4706 Tier II
6 NC_000002.12 2 48560139 - STON1-GTF2A1L NNNAGGTCTTAACAGTTCAG NGG 2 0.4706 Tier II
7 NC_000002.12 2 238336313 + TRAF3IP1 NNNAGCTATCAACAGCTCAG NGG 2 0.4333 Tier II
8 NC_000011.10 11 84482559 - DLG2 NNNACCTCTCAACAGTTCTG NGG 2 0.4231 Tier II
9 NC_000003.12 3 174512459 - NAALADL2 NNNAGTTCTCAACAGTTCAG NAG 2 0.2407 Tier II
10 NC_000006.12 6 38307879 + BTBD9 NNNAGCTCTAAACACTTCAG NGG 2 0.2364 Tier II
11 NC_000013.11 13 51359171 + INTS6 NNNAGCTGTCAACACTTCAG NGG 2 0.1753 Tier II
12 NC_000013.11 13 51359171 + SERPINE3 NNNAGCTGTCAACACTTCAG NGG 2 0.1753 Tier II
13 NC_000003.12 3 10384396 + ATP2B2 NNNGGCTCTCAACAGTTCAG NAG 2 0.162 Tier II
14 NC_000009.12 9 28193721 + LINGO2 NNNATCTCTCAACAGTTCTG NGG 2 0.1615 Tier II
15 NC_000007.14 7 2124139 + MAD1L1 NNNAGCTCTCCACAGGTCAG NGG 2 0.1385 Tier II
16 NC_000001.11 1 175725542 + TNR NNNAGCTCTCAATAGTTCAG NGA 2 0.0486 Tier II
17 NC_000007.14 7 146896516 - CNTNAP2 NNNAGCTCTCAACAGTTCAG NGT 1 0.0161 Tier II
18 NC_000002.12 2 77999180 + LOC101927967 NNNAGCTCTTAACAGCTCAG NGG 2 0.6275 Tier III
19 NC_000005.10 5 125136015 - LOC101927421 NNNGGCTCTCAACAGTACAG NGG 2 0.3333 Tier III
20 NC_000003.12 3 106564553 - LOC101929485 NNNAGCTCTCATCAGTTTAG NGG 2 0.2143 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 142111095 + Gucy2f NNNGGCTCTTAACAGTTCAG NGG 2 0.5882 Tier I
2 NC_000071.6 5 100446534 - Lin54 NNNAGCTCTCAACAGTGCAG NAG 2 0.0305 Tier I
3 NC_000083.6 17 30871921 - Dnah8 NNNAGCTCCCAACAGTTCAG NGG 1 0.9231 Tier II
4 NC_000071.6 5 21022199 - Ptpn12 NNNAGCTGACAACAGTTCAG NGG 2 0.5969 Tier II
5 NC_000072.6 6 138457210 + Lmo3 NNNAGCTCTCAGCAGCTCAG NGG 2 0.4815 Tier II
6 NC_000082.6 16 44312292 + Sidt1 NNNAGTTCTCAACAGTTCAC NGG 2 0.398 Tier II
7 NC_000067.6 1 87026393 + Dis3l2 NNNAGCTCTCAACTGTTCAT NGG 2 0.3733 Tier II
8 NC_000081.6 15 33520353 - Cpq NNNAGCTCTCAAAAATTCAG NGG 2 0.362 Tier II
9 NC_000076.6 10 25251529 + Akap7 NNNAACTCTCCACAGTTCAG NGG 2 0.3467 Tier II
10 NC_000076.6 10 43981552 + Crybg1 NNNAGGTCTCAACAGCTCAG NGG 2 0.3333 Tier II
11 NC_000084.6 18 66219495 - Ccbe1 NNNAGCTCACATCAGTTCAG NGG 2 0.3095 Tier II
12 NC_000078.6 12 102308738 - Rin3 NNNAGCTCTCAACAGTCCAC NGG 2 0.3025 Tier II
13 NC_000076.6 10 59546453 + Mcu NNNAGCTCTCAACTGTTCTG NGG 2 0.2872 Tier II
14 NC_000069.6 3 138744626 - Tspan5 NNNAGCTCTCCACAGTCCAG NGG 2 0.2824 Tier II
15 NC_000071.6 5 130203931 - Rabgef1 NNNAGCTCACACCAGTTCAG NGG 2 0.2444 Tier II
16 NC_000076.6 10 40420332 - Cdk19 NNNAGCTCTCAACAATTCCG NGG 2 0.1947 Tier II
17 NC_000071.6 5 76730101 + Cracd NNNAGCTCTCAACACTCCAG NGG 2 0.1925 Tier II
18 NC_000073.6 7 54979876 - Luzp2 NNNATCTCTCAACAGTTCTG NGG 2 0.1615 Tier II
19 NC_000079.6 13 73261611 + Irx4 NNNAGCTCTCACCAGTTCTG NGG 2 0.1417 Tier II
20 NC_000067.6 1 146647323 + Brinp3 NNNAGCTCTGAACACTTCAG NGG 2 0.1061 Tier II
21 NC_000080.6 14 93761692 - Pcdh9 NNNAGCTCTCAACAGTTCAA NTG 2 0.0365 Tier II
22 NC_000070.6 4 36453985 - Lingo2 NNNAGCTCTCAAGATTTCAG NGG 2 0.0195 Tier II
23 NC_000080.6 14 12643818 - Cadps NNNAGCTTTCAACAGTTCAG NGC 2 0.0194 Tier II
24 NC_000074.6 8 29112262 + Unc5d NNNAGCTCTCAACAGTTCAC NTG 2 0.0167 Tier II
25 NC_000071.6 5 124725503 + Dnah10 NNNAGCTCTCAACAGTGCAG NGT 2 0.0019 Tier II
26 NC_000076.6 10 25233752 + Akap7 NNNAGCTCTCAACAGTTCAG NTT 2 0.0 Tier II
27 NC_000079.6 13 48018159 + 4931429P17Rik NNNAGCTCTAAATAGTTCAG NGG 2 0.6067 Tier III
28 NC_000068.7 2 34646291 + Gm34372 NNNTGCTCTTAACAGTTCAG NGG 2 0.5989 Tier III
29 NC_000079.6 13 71084490 + Gm40997 NNNAGATGTCAACAGTTCAG NGG 2 0.5969 Tier III
30 NC_000082.6 16 73229003 + 4930500H12Rik NNNAGCAATCAACAGTTCAG NGG 2 0.5688 Tier III
31 NC_000072.6 6 138457210 + Gm30332 NNNAGCTCTCAGCAGCTCAG NGG 2 0.4815 Tier III
32 NC_000076.6 10 25251529 + Gm7791 NNNAACTCTCCACAGTTCAG NGG 2 0.3467 Tier III
33 NC_000076.6 10 43981552 + Gm40636 NNNAGGTCTCAACAGCTCAG NGG 2 0.3333 Tier III
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Other clones with same target sequence:

(none)