Construct: sgRNA BRDN0001147947

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGAGTTTGAATGGTCCGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRAG1 (157285)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75793

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 8376421 + PRAG1 NNNAGTTTGAATGGTCCGTG NGG 0 1.0 Tier I
2 NC_000002.12 2 127955217 + SAP130 NNNAGTTGGAATGGTCGGTG NGG 2 0.0431 Tier I
3 NC_000022.11 22 21730528 - YPEL1 NNNAGTTTGAATAGTCAGTG NGG 2 0.4308 Tier II
4 NC_000008.11 8 66501727 - VXN NNNAATTTGAATGGTCTGTG NGG 2 0.4044 Tier II
5 NC_000004.12 4 38794486 - TLR1 NNNAGTTTGACTGGACCGTG NGG 2 0.2316 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 73203105 - Gm29185 NNNAGTTTGATTGGTCCTTG NGG 2 0.2051 Tier III
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Other clones with same target sequence:

(none)