Construct: sgRNA BRDN0001147953

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCCTGCAAAAACTCAACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C3 (5289)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76965

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 42020684 + PIK3C3 NNNCTGCAAAAACTCAACAC NGG 0 1.0 Tier I
2 NC_000002.12 2 140443365 - LRP1B NNNCCTCAAAAACTCAACAC NGG 2 0.6667 Tier II
3 NC_000005.10 5 127055716 + C5orf63 NNNTTTCAAAAACTCAACAC NGG 2 0.5333 Tier II
4 NC_000002.12 2 1887986 + MYT1L NNNCTGCAAAGATTCAACAC NGG 2 0.455 Tier II
5 NC_000009.12 9 28925451 + LINGO2 NNNCAGTAAAAACTCAACAC NGG 2 0.4063 Tier II
6 NC_000002.12 2 210491229 - CPS1 NNNCTGCAGGAACTCAACAC NGG 2 0.3704 Tier II
7 NC_000017.11 17 17151469 - MPRIP NNNCTCCTAAAACTCAACAC NGG 2 0.2922 Tier II
8 NC_000018.10 18 5991819 + L3MBTL4 NNNCTGAAAAAAATCAACAC NGG 2 0.2885 Tier II
9 NC_000005.10 5 168073597 - TENM2 NNNATGCAACAACTCAACAC NGG 2 0.2807 Tier II
10 NC_000008.11 8 23209458 + TNFRSF10A NNNCTGCAGACACTCAACAC NGG 2 0.2667 Tier II
11 NC_000003.12 3 10435927 - ATP2B2 NNNCAGCCAAAACTCAACAC NGG 2 0.2143 Tier II
12 NC_000008.11 8 132463571 + KCNQ3 NNNTTGCAAAAACTCAGCAC NGG 2 0.1412 Tier II
13 NC_000013.11 13 95681245 + DNAJC3 NNNCTGCCAAAACCCAACAC NGG 2 0.1224 Tier II
14 NC_000004.12 4 8080389 + ABLIM2 NNNCTTCAAAAACTCAGCAC NGG 2 0.1176 Tier II
15 NC_000001.11 1 232994198 + PCNX2 NNNCTGCAAACACTCAACAC NAG 2 0.1037 Tier II
16 NC_000009.12 9 83216944 + RASEF NNNCTGCAAACACTCAACAC NAG 2 0.1037 Tier II
17 NC_000011.10 11 88695883 - GRM5 NNNCTGCAAACACTCAACCC NGG 2 0.0828 Tier II
18 NC_000002.12 2 241615286 + THAP4 NNNCTGCCAAAACTCACCAC NGG 2 0.0756 Tier II
19 NC_000013.11 13 93655322 - GPC6 NNNCTGCTAAAACTCAGCAC NGG 2 0.0756 Tier II
20 NC_000019.10 19 17775742 - FCHO1 NNNCTGCAAAACCCCAACAC NGG 2 0.0752 Tier II
21 NC_000007.14 7 64048948 - ZNF727 NNNCTGCAAAAACCCAGCAC NGG 2 0.0504 Tier II
22 NC_000004.12 4 84740042 + WDFY3 NNNCTGCAAAAATTCAACAC NTG 2 0.0273 Tier II
23 NC_000012.12 12 12418975 - BORCS5 NNNCTGCAAAAAATCAACAC NGA 2 0.0267 Tier II
24 NC_000002.12 2 42185829 - EML4 NNNCTGCAAAAACTCCACAC NGA 2 0.0 Tier II
25 NC_000015.10 15 100403912 - CERS3 NNNCTGCAAAAACTTTACAC NGG 2 0.0 Tier II
26 NC_000004.12 4 127295670 - LOC102724210 NNNCTGCAGAAACTCAACAC NAG 2 0.1728 Tier III
27 NC_000018.10 18 39646322 - MIR924HG NNNCTGCAAAAACTCATCAC NGG 1 0.1333 Tier III
28 NC_000007.14 7 77419524 - LOC101927243 NNNCTGCAACAAATCAACAC NGG 2 0.1282 Tier III
29 NC_000014.9 14 39497219 + LOC105370460 NNNCTGCAAACACTCAACAC NAG 2 0.1037 Tier III
30 NC_000014.9 14 39497219 + LOC105370461 NNNCTGCAAACACTCAACAC NAG 2 0.1037 Tier III
31 NC_000018.10 18 45703166 - LOC105372093 NNNCTGCAAACACTCAACAC NAG 2 0.1037 Tier III
32 NC_000005.10 5 130339999 + LOC105379171 NNNCTGCTAAAACTCAACCC NGG 2 0.0887 Tier III
33 NC_000018.10 18 991755 + LOC107985165 NNNCTGAAAAAACTCAACAC NGA 2 0.0521 Tier III
34 NC_000004.12 4 63480864 - LOC105377253 NNNCTGCAAAAACTCAACAT NGA 2 0.0208 Tier III
35 NC_000010.11 10 78443958 - LOC107984245 NNNCTGCAAAAACGCAATAC NGG 2 0.0 Tier III
36 NC_000015.10 15 100403912 - CERS3-AS1 NNNCTGCAAAAACTTTACAC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 30305021 + Pik3c3 NNNCTGTAAGAACTCAACAC NGG 2 0.4514 Tier I
2 NC_000079.6 13 36946472 + F13a1 NNNCAGCAAAAACCCAACAC NGG 2 0.1429 Tier I
3 NC_000077.6 11 93965787 + Nme1 NNNCTCCAAAGACTCAACAC NGG 2 0.4432 Tier II
4 NC_000075.6 9 29357714 - Ntm NNNTTGGAAAAACTCAACAC NGG 2 0.3765 Tier II
5 NC_000070.6 4 149366531 + Ube4b NNNCTGAAAACACTCAACAC NGG 2 0.3 Tier II
6 NC_000067.6 1 24564090 + Col19a1 NNNATGCAAAATCTCAACAC NGG 2 0.2807 Tier II
7 NC_000073.6 7 35743649 + Dpy19l3 NNNCTGCAGACACTCAACAC NGG 2 0.2667 Tier II
8 NC_000085.6 19 7107903 + Macrod1 NNNATGCAAAAACCCAACAC NGG 2 0.2406 Tier II
9 NC_000085.6 19 7107903 + Flrt1 NNNATGCAAAAACCCAACAC NGG 2 0.2406 Tier II
10 NC_000075.6 9 53336688 + Exph5 NNNATGCAAAAACTCAACAC NAG 2 0.2183 Tier II
11 NC_000081.6 15 32313267 - Sema5a NNNCTTCAAAAACTCAACAC NAG 2 0.1728 Tier II
12 NC_000074.6 8 41059403 - Mtus1 NNNCTGCAAAAATTAAACAC NGG 2 0.1556 Tier II
13 NC_000076.6 10 83272525 + Slc41a2 NNNCTGCAAAAACTCAACTC NAG 2 0.1396 Tier II
14 NC_000067.6 1 55618030 - Plcl1 NNNCTGCCAAACCTCAACAC NGG 2 0.1128 Tier II
15 NC_000077.6 11 67602925 - Gas7 NNNCTGCAAAAATTCATCAC NGG 2 0.0933 Tier II
16 NC_000069.6 3 108719823 + Gpsm2 NNNCTGCAAAAACTCAAAAC NGA 2 0.0374 Tier II
17 NC_000081.6 15 12322330 - Golph3 NNNCTGCAAAAACTCAATAC NTG 2 0.025 Tier II
18 NC_000075.6 9 74992909 + Fam214a NNNCTGCAAAAACTCAAAAC NTG 2 0.021 Tier II
19 NC_000085.6 19 55608635 + Vti1a NNNCTGCAAAAACTCAACAC NTC 2 0.0 Tier II
20 NC_000068.7 2 117853858 - 4930412B13Rik NNNCTGCAAATACTCAAAAC NGG 2 0.1657 Tier III
21 NC_000074.6 8 41059403 - Gm16193 NNNCTGCAAAAATTAAACAC NGG 2 0.1556 Tier III
22 NC_000079.6 13 36946472 + Gm30489 NNNCAGCAAAAACCCAACAC NGG 2 0.1429 Tier III
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Other clones with same target sequence:

(none)