Construct: sgRNA BRDN0001147954
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCAGGCACTGTTACCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKMT1B (1159)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76592
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
43594366 |
- |
CKMT1B |
NNNAGGCACTGTTACCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43694198 |
- |
CKMT1A |
NNNAGGCACTGTTACCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000002.12 |
2 |
54288717 |
- |
ACYP2 |
NNNAGGCACCGTTACATCGG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000001.11 |
1 |
246186871 |
+ |
SMYD3 |
NNNAGGCACAGTTACCTCGC |
NGG |
2 |
0.3673 |
Tier II |
| 5 |
NC_000017.11 |
17 |
49050545 |
+ |
IGF2BP1 |
NNNCGGCACTGTTACCTTGG |
NGG |
2 |
0.2269 |
Tier II |
| 6 |
NC_000005.10 |
5 |
178365331 |
+ |
COL23A1 |
NNNAGGCACTGAGACCTCGG |
NGG |
2 |
0.2087 |
Tier II |
| 7 |
NC_000008.11 |
8 |
23387305 |
+ |
LOXL2 |
NNNAGGCACTGTGTCCTCGG |
NGG |
2 |
0.1391 |
Tier II |
| 8 |
NC_000002.12 |
2 |
158270798 |
+ |
CCDC148 |
NNNAGGCACTGTTACCAGGG |
NGG |
2 |
0.0711 |
Tier II |
| 9 |
NC_000001.11 |
1 |
246186871 |
+ |
LOC124904573 |
NNNAGGCACAGTTACCTCGC |
NGG |
2 |
0.3673 |
Tier III |
| 10 |
NC_000005.10 |
5 |
178365331 |
+ |
LOC105377757 |
NNNAGGCACTGAGACCTCGG |
NGG |
2 |
0.2087 |
Tier III |
| 11 |
NC_000006.12 |
6 |
28897268 |
- |
HCG14 |
NNNAGGCACTGTTACCTGGA |
NGG |
2 |
0.125 |
Tier III |
| 12 |
NC_000017.11 |
17 |
74212800 |
- |
MGC16275 |
NNNAGGCACTGTTACATCGG |
NGA |
2 |
0.0694 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
49671195 |
- |
Nol8 |
NNNAGACACTGTTACCTTGG |
NGG |
2 |
0.6429 |
Tier II |
| 2 |
NC_000068.7 |
2 |
131154703 |
- |
1700037H04Rik |
NNNAGGCACTGCTGCCTCGG |
NGG |
2 |
0.4421 |
Tier II |
| 3 |
NC_000077.6 |
11 |
69833831 |
+ |
Nlgn2 |
NNNAGGCACCCTTACCTCGG |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000069.6 |
3 |
144504213 |
+ |
Hs2st1 |
NNNAGGCTCTCTTACCTCGG |
NGG |
2 |
0.1837 |
Tier II |
| 5 |
NC_000069.6 |
3 |
144504213 |
+ |
Gm43707 |
NNNAGGCTCTCTTACCTCGG |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000068.7 |
2 |
58299280 |
- |
Acvr1c |
NNNAGGCACTGTGCCCTCGG |
NGG |
2 |
0.0559 |
Tier II |
| 7 |
NC_000086.7 |
X |
162811794 |
- |
Txlng |
NNNAGGCACTGTTACCTCAG |
NGC |
2 |
0.0159 |
Tier II |
| 8 |
NC_000073.6 |
7 |
79715341 |
- |
Kif7 |
NNNAGGCACTGTTACCTCTG |
NGT |
2 |
0.0108 |
Tier II |
| 9 |
NC_000077.6 |
11 |
52479237 |
- |
Gm12209 |
NNNAGGTACTGTTACCTCAG |
NGG |
2 |
0.5804 |
Tier III |
| 10 |
NC_000078.6 |
12 |
113356793 |
- |
Igh |
NNNGGGCACTGTTACCTCAG |
NGG |
2 |
0.4464 |
Tier III |
Other clones with same target sequence:
(none)
