Construct: sgRNA BRDN0001147956

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTCATACAGGATGCAACCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK3 (4752)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77359

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 52148453 + NEK3 NNNATACAGGATGCAACCCA NGG 0 1.0 Tier I
2 NC_000015.10 15 101061058 + LRRK1 NNNACACAGGATGCAACCCT NGG 2 0.6 Tier II
3 NC_000003.12 3 10327911 + ATP2B2 NNNATCCAGGATGCAACCCA NGG 1 0.4545 Tier II
4 NC_000011.10 11 132620551 + OPCML NNNAAACAGGAAGCAACCCA NGG 2 0.4 Tier II
5 NC_000002.12 2 137632176 - THSD7B NNNATATAGCATGCAACCCA NGG 2 0.325 Tier II
6 NC_000007.14 7 2025611 + MAD1L1 NNNATACAGGTTGCAACACA NGG 2 0.1657 Tier II
7 NC_000004.12 4 149459227 + IQCM NNNATACAGGATGAAACCAA NGG 2 0.15 Tier II
8 NC_000010.11 10 48610382 - ARHGAP22 NNNATACAGGTTGCAATCCA NGG 2 0.1436 Tier II
9 NC_000023.11 X 110206750 - AMMECR1 NNNAAACAGGATGCCACCCA NGG 2 0.1364 Tier II
10 NC_000024.10 Y 6943247 + TBL1Y NNNATACAGGAAGCAACCCA NGC 2 0.0178 Tier II
11 NC_000015.10 15 101061058 + LOC105371026 NNNACACAGGATGCAACCCT NGG 2 0.6 Tier III
12 NC_000009.12 9 89803188 - LOC107987032 NNNAAACATGATGCAACCCA NGG 2 0.2667 Tier III
13 NC_000009.12 9 84461214 - LOC102724036 NNNATACATCATGCAACCCA NGG 2 0.2133 Tier III
14 NC_000009.12 9 84461214 - LOC124902192 NNNATACATCATGCAACCCA NGG 2 0.2133 Tier III
15 NC_000007.14 7 142763631 - TRB NNNAGACAGGATGCAGCCCA NGG 2 0.1231 Tier III
16 NC_000007.14 7 142763631 - PRSS3P1 NNNAGACAGGATGCAGCCCA NGG 2 0.1231 Tier III
17 NC_000016.10 16 86492235 + FENDRR NNNATAAAGGATGCAACCCA NTG 2 0.0292 Tier III
18 NC_000002.12 2 64612164 + LINC02579 NNNATACAGGATGCCAGCCA NGG 2 0.016 Tier III
19 NC_000015.10 15 25093750 + SNHG14 NNNATACAGGATGCACCACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 74609137 - Stk36 NNNATACAGGATGCAGCCCA NAG 2 0.0499 Tier I
2 NC_000074.6 8 22149899 + Nek3 NNNATACAGAATGCATCCCA NGG 2 0.0 Tier I
3 NC_000068.7 2 44900180 + Gtdc1 NNNATACGGGAAGCAACCCA NGG 2 0.5867 Tier II
4 NC_000086.7 X 151835470 - Huwe1 NNNGTTCAGGATGCAACCCA NGG 2 0.4464 Tier II
5 NC_000079.6 13 77781320 + Fam172a NNNATACAAGATGCAACTCA NGG 2 0.4133 Tier II
6 NC_000072.6 6 76904857 + Ctnna2 NNNATGCAGGATGCAATCCA NGG 2 0.3333 Tier II
7 NC_000077.6 11 35813175 - Rars NNNATACAGAATGAAACCCA NGG 2 0.3267 Tier II
8 NC_000072.6 6 28692252 - Snd1 NNNATACAGGAGGCAACACA NGG 2 0.2692 Tier II
9 NC_000079.6 13 20451675 - Elmo1 NNNAAACAGGATGCAAACCA NGG 2 0.2333 Tier II
10 NC_000067.6 1 192693318 - Hhat NNNATCCAGGATCCAACCCA NGG 2 0.1914 Tier II
11 NC_000077.6 11 78055391 - Flot2 NNNAGACAGGATGCAACCCA NAG 2 0.1659 Tier II
12 NC_000067.6 1 184052254 - Dusp10 NNNATAAAGGATGCAAGCCA NGG 2 0.0441 Tier II
13 NC_000075.6 9 62747818 + Itga11 NNNAAACAGGATGCAAGCCA NGG 2 0.0294 Tier II
14 NC_000067.6 1 38118882 - Rev1 NNNATACAGGATGTAACCCA NGT 2 0.0118 Tier II
15 NC_000075.6 9 119560920 + Scn5a NNNAAACAGGATGGAACCCA NGG 2 0.0 Tier II
16 NC_000082.6 16 26027286 + P3h2 NNNATACAGAATGCATCCCA NGG 2 0.0 Tier II
17 NC_000071.6 5 84370355 + Epha5 NNNATACAGGATGGATCCCA NGG 2 0.0 Tier II
18 NC_000077.6 11 75884823 - Rph3al NNNCTACAGGATGCACCCCA NGG 2 0.0 Tier II
19 NC_000068.7 2 17008662 + 4930515L03Rik NNNACACAGGAAGCAACCCA NGG 2 0.8 Tier III
20 NC_000079.6 13 20451675 - Gm32036 NNNAAACAGGATGCAAACCA NGG 2 0.2333 Tier III
21 NC_000079.6 13 15786598 + 4933412O06Rik NNNATAGAGCATGCAACCCA NGG 2 0.1882 Tier III
22 NC_000067.6 1 73228103 - Gm29185 NNNATACAGGATGCAACCGA NAG 2 0.0324 Tier III
23 NC_000068.7 2 63544999 - Gm8541 NNNAAACAGGATGCATCCCA NGG 2 0.0 Tier III
24 NC_000072.6 6 69778627 - Igk NNNATACAGGATGGATCCCA NGG 2 0.0 Tier III
25 NC_000072.6 6 69826224 - Igk NNNATACAGGATGGATCCCA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)