Construct: sgRNA BRDN0001147957
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGATGATAACAGCTAAGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKRA (8575)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76300
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
178450387 |
- |
PRKRA |
NNNTGATAACAGCTAAGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
112212641 |
- |
ZC3H8 |
NNNTGATAACAGCTGAGCCA |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000002.12 |
2 |
112212641 |
- |
FBLN7 |
NNNTGATAACAGCTGAGCCA |
NGG |
1 |
0.65 |
Tier II |
| 4 |
NC_000001.11 |
1 |
81960413 |
- |
ADGRL2 |
NNNTGATAACATCTAAACCA |
NGG |
2 |
0.359 |
Tier II |
| 5 |
NC_000002.12 |
2 |
10750098 |
+ |
ATP6V1C2 |
NNNTGATCACAGCTAAGTCA |
NGG |
2 |
0.2755 |
Tier II |
| 6 |
NC_000005.10 |
5 |
141858751 |
+ |
PCDH1 |
NNNTGAAAACAGCTAAGCCC |
NGG |
2 |
0.1989 |
Tier II |
| 7 |
NC_000003.12 |
3 |
94089278 |
- |
NSUN3 |
NNNTGATAACAGCTAAGTCA |
NCG |
2 |
0.0689 |
Tier II |
| 8 |
NC_000007.14 |
7 |
128247719 |
+ |
LEP |
NNNTGATAACAGCTAAGGCA |
NTG |
2 |
0.0052 |
Tier II |
| 9 |
NC_000001.11 |
1 |
59542038 |
+ |
FGGY |
NNNTGATAACAGCTAAGCCA |
NTT |
2 |
0.0 |
Tier II |
| 10 |
NC_000006.12 |
6 |
161877987 |
+ |
PRKN |
NNNTGATAACAGCTAAGCCA |
NCC |
2 |
0.0 |
Tier II |
| 11 |
NC_000010.11 |
10 |
44340377 |
- |
LOC124902544 |
NNNTGAAAACAGCTCAGCCA |
NGG |
2 |
0.2386 |
Tier III |
| 12 |
NC_000005.10 |
5 |
133951135 |
- |
LOC105379182 |
NNNTGATAACAGCTAACACA |
NGG |
2 |
0.1267 |
Tier III |
| 13 |
NC_000010.11 |
10 |
2985366 |
+ |
LOC105376352 |
NNNTGATCACAGCTCAGCCA |
NGG |
2 |
0.1169 |
Tier III |
| 14 |
NC_000022.11 |
22 |
21080725 |
+ |
LOC112268300 |
NNNGGATAACAGCGAAGCCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
76647270 |
- |
Prkra |
NNNTGATAACAGCTAAGCCT |
NGG |
1 |
0.6 |
Tier I |
| 2 |
NC_000075.6 |
9 |
60235153 |
- |
Thsd4 |
NNNTAAAAACAGCTAAGCCA |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000073.6 |
7 |
119845757 |
- |
Rexo5 |
NNNTGATAACAGCTAAGCCT |
NGG |
1 |
0.6 |
Tier II |
| 4 |
NC_000073.6 |
7 |
29313725 |
+ |
Dpf1 |
NNNTGATGACAGCTGAGCCA |
NGG |
2 |
0.4767 |
Tier II |
| 5 |
NC_000067.6 |
1 |
25795804 |
- |
Adgrb3 |
NNNTCATAACAGCTAAGACA |
NGG |
2 |
0.4231 |
Tier II |
| 6 |
NC_000081.6 |
15 |
103205789 |
- |
Cbx5 |
NNNTGAAAACAGCTAAGCTA |
NGG |
2 |
0.4038 |
Tier II |
| 7 |
NC_000074.6 |
8 |
65747848 |
+ |
Marchf1 |
NNNTGATAACAGCTAAACAA |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000081.6 |
15 |
102593190 |
- |
Atf7 |
NNNTGCTAACAGCTAAGTCA |
NGG |
2 |
0.2922 |
Tier II |
| 9 |
NC_000081.6 |
15 |
58026476 |
+ |
9130401M01Rik |
NNNAGATAACACCTAAGCCA |
NGG |
2 |
0.2521 |
Tier II |
| 10 |
NC_000067.6 |
1 |
158669554 |
+ |
Astn1 |
NNNTGATAACATCTAAGTCA |
NGG |
2 |
0.2473 |
Tier II |
| 11 |
NC_000070.6 |
4 |
127223774 |
+ |
Dlgap3 |
NNNTGATAGCAGCCAAGCCA |
NGG |
2 |
0.1905 |
Tier II |
| 12 |
NC_000074.6 |
8 |
10016983 |
- |
Tnfsf13b |
NNNTTATAACAGCTAAGCTA |
NGG |
2 |
0.1385 |
Tier II |
| 13 |
NC_000076.6 |
10 |
108243653 |
- |
Ppp1r12a |
NNNTGAGAACAGCTAACCCA |
NGG |
2 |
0.1384 |
Tier II |
| 14 |
NC_000070.6 |
4 |
106777805 |
+ |
Acot11 |
NNNTGAGAACAGCTAAGCCC |
NGG |
2 |
0.1337 |
Tier II |
| 15 |
NC_000081.6 |
15 |
100105523 |
- |
Dip2b |
NNNTGATCACAGCCAAGCCA |
NGG |
2 |
0.1224 |
Tier II |
| 16 |
NC_000075.6 |
9 |
59701532 |
+ |
Gramd2 |
NNNTGCTAACAGCTAAGGCA |
NGG |
2 |
0.0606 |
Tier II |
| 17 |
NC_000067.6 |
1 |
134554633 |
- |
Mgat4e |
NNNTGATAACAGCTGAGCCA |
NGA |
2 |
0.0451 |
Tier II |
| 18 |
NC_000076.6 |
10 |
62703920 |
- |
Stox1 |
NNNTGGTAACAGCTAAGCCA |
NGC |
2 |
0.0159 |
Tier II |
| 19 |
NC_000079.6 |
13 |
63325354 |
- |
Fancc |
NNNTGATAACAGCTTTGCCA |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000079.6 |
13 |
63325354 |
- |
Aopep |
NNNTGATAACAGCTTTGCCA |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000068.7 |
2 |
68822496 |
+ |
Gm36174 |
NNNTGCTAACAGCTGAGCCA |
NGG |
2 |
0.2955 |
Tier III |
| 22 |
NC_000068.7 |
2 |
68822496 |
+ |
Gm36231 |
NNNTGCTAACAGCTGAGCCA |
NGG |
2 |
0.2955 |
Tier III |
Other clones with same target sequence:
(none)
