Construct: sgRNA BRDN0001147957

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGATGATAACAGCTAAGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKRA (8575)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76300

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 178450387 - PRKRA NNNTGATAACAGCTAAGCCA NGG 0 1.0 Tier I
2 NC_000002.12 2 112212641 - ZC3H8 NNNTGATAACAGCTGAGCCA NGG 1 0.65 Tier II
3 NC_000002.12 2 112212641 - FBLN7 NNNTGATAACAGCTGAGCCA NGG 1 0.65 Tier II
4 NC_000001.11 1 81960413 - ADGRL2 NNNTGATAACATCTAAACCA NGG 2 0.359 Tier II
5 NC_000002.12 2 10750098 + ATP6V1C2 NNNTGATCACAGCTAAGTCA NGG 2 0.2755 Tier II
6 NC_000005.10 5 141858751 + PCDH1 NNNTGAAAACAGCTAAGCCC NGG 2 0.1989 Tier II
7 NC_000003.12 3 94089278 - NSUN3 NNNTGATAACAGCTAAGTCA NCG 2 0.0689 Tier II
8 NC_000007.14 7 128247719 + LEP NNNTGATAACAGCTAAGGCA NTG 2 0.0052 Tier II
9 NC_000001.11 1 59542038 + FGGY NNNTGATAACAGCTAAGCCA NTT 2 0.0 Tier II
10 NC_000006.12 6 161877987 + PRKN NNNTGATAACAGCTAAGCCA NCC 2 0.0 Tier II
11 NC_000010.11 10 44340377 - LOC124902544 NNNTGAAAACAGCTCAGCCA NGG 2 0.2386 Tier III
12 NC_000005.10 5 133951135 - LOC105379182 NNNTGATAACAGCTAACACA NGG 2 0.1267 Tier III
13 NC_000010.11 10 2985366 + LOC105376352 NNNTGATCACAGCTCAGCCA NGG 2 0.1169 Tier III
14 NC_000022.11 22 21080725 + LOC112268300 NNNGGATAACAGCGAAGCCA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 76647270 - Prkra NNNTGATAACAGCTAAGCCT NGG 1 0.6 Tier I
2 NC_000075.6 9 60235153 - Thsd4 NNNTAAAAACAGCTAAGCCA NGG 2 0.7583 Tier II
3 NC_000073.6 7 119845757 - Rexo5 NNNTGATAACAGCTAAGCCT NGG 1 0.6 Tier II
4 NC_000073.6 7 29313725 + Dpf1 NNNTGATGACAGCTGAGCCA NGG 2 0.4767 Tier II
5 NC_000067.6 1 25795804 - Adgrb3 NNNTCATAACAGCTAAGACA NGG 2 0.4231 Tier II
6 NC_000081.6 15 103205789 - Cbx5 NNNTGAAAACAGCTAAGCTA NGG 2 0.4038 Tier II
7 NC_000074.6 8 65747848 + Marchf1 NNNTGATAACAGCTAAACAA NGG 2 0.4 Tier II
8 NC_000081.6 15 102593190 - Atf7 NNNTGCTAACAGCTAAGTCA NGG 2 0.2922 Tier II
9 NC_000081.6 15 58026476 + 9130401M01Rik NNNAGATAACACCTAAGCCA NGG 2 0.2521 Tier II
10 NC_000067.6 1 158669554 + Astn1 NNNTGATAACATCTAAGTCA NGG 2 0.2473 Tier II
11 NC_000070.6 4 127223774 + Dlgap3 NNNTGATAGCAGCCAAGCCA NGG 2 0.1905 Tier II
12 NC_000074.6 8 10016983 - Tnfsf13b NNNTTATAACAGCTAAGCTA NGG 2 0.1385 Tier II
13 NC_000076.6 10 108243653 - Ppp1r12a NNNTGAGAACAGCTAACCCA NGG 2 0.1384 Tier II
14 NC_000070.6 4 106777805 + Acot11 NNNTGAGAACAGCTAAGCCC NGG 2 0.1337 Tier II
15 NC_000081.6 15 100105523 - Dip2b NNNTGATCACAGCCAAGCCA NGG 2 0.1224 Tier II
16 NC_000075.6 9 59701532 + Gramd2 NNNTGCTAACAGCTAAGGCA NGG 2 0.0606 Tier II
17 NC_000067.6 1 134554633 - Mgat4e NNNTGATAACAGCTGAGCCA NGA 2 0.0451 Tier II
18 NC_000076.6 10 62703920 - Stox1 NNNTGGTAACAGCTAAGCCA NGC 2 0.0159 Tier II
19 NC_000079.6 13 63325354 - Fancc NNNTGATAACAGCTTTGCCA NGG 2 0.0 Tier II
20 NC_000079.6 13 63325354 - Aopep NNNTGATAACAGCTTTGCCA NGG 2 0.0 Tier II
21 NC_000068.7 2 68822496 + Gm36174 NNNTGCTAACAGCTGAGCCA NGG 2 0.2955 Tier III
22 NC_000068.7 2 68822496 + Gm36231 NNNTGCTAACAGCTGAGCCA NGG 2 0.2955 Tier III
Download CSV

Other clones with same target sequence:

(none)