Construct: sgRNA BRDN0001147960
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGGACCGCATGGTGCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS1 (5631)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76199
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
107645266 |
+ |
PRPS1 |
NNNGGACCGCATGGTGCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
55523905 |
- |
XKR4 |
NNNGGACCGTAGGGTGCTTG |
NGG |
2 |
0.4706 |
Tier I |
| 3 |
NC_000008.11 |
8 |
143726303 |
+ |
FAM83H |
NNNGGACCGCACGGTGCTTC |
NGG |
2 |
0.406 |
Tier I |
| 4 |
NC_000019.10 |
19 |
55614890 |
- |
ZNF865 |
NNNGGGCCGCCTGGTGCTTG |
NGG |
2 |
0.2857 |
Tier I |
| 5 |
NC_000011.10 |
11 |
117192833 |
+ |
SIDT2 |
NNNGGACCGCATGGTGCTGC |
NGG |
2 |
0.1071 |
Tier I |
| 6 |
NC_000008.11 |
8 |
144116091 |
- |
WDR97 |
NNNGGACCGCATGGGGCCTG |
NGG |
2 |
0.0214 |
Tier I |
| 7 |
NC_000014.9 |
14 |
47383934 |
+ |
MDGA2 |
NNNGGACTGCATGGTGCTTA |
NGG |
2 |
0.8203 |
Tier II |
| 8 |
NC_000009.12 |
9 |
125151254 |
+ |
PPP6C |
NNNCGACCGCATGGTGCTTG |
NGG |
1 |
0.5294 |
Tier II |
| 9 |
NC_000014.9 |
14 |
93786012 |
+ |
PRIMA1 |
NNNGGAGTGCATGGTGCTTG |
NGG |
2 |
0.4118 |
Tier II |
| 10 |
NC_000010.11 |
10 |
124043665 |
+ |
CHST15 |
NNNGGACCGCAGGGTGCATG |
NGG |
2 |
0.3333 |
Tier II |
| 11 |
NC_000020.11 |
20 |
40686119 |
- |
MAFB |
NNNGAACCGCATGGTGCTTG |
NAG |
2 |
0.2247 |
Tier II |
| 12 |
NC_000002.12 |
2 |
150486668 |
- |
RND3 |
NNNGGACCGCGTGGCGCTTG |
NGG |
2 |
0.1773 |
Tier II |
| 13 |
NC_000001.11 |
1 |
41548236 |
- |
HIVEP3 |
NNNGGACTGCATGGTGCTTG |
NGA |
2 |
0.0608 |
Tier II |
| 14 |
NC_000019.10 |
19 |
1495651 |
+ |
REEP6 |
NNNGGAGCGCATGGGGCTTG |
NGG |
2 |
0.0235 |
Tier II |
| 15 |
NC_000002.12 |
2 |
77434007 |
- |
LRRTM4 |
NNNGAACCGCATGGTTCTTG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000006.12 |
6 |
166565539 |
- |
RPS6KA2 |
NNNGGACCACATGGTTCTTG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000009.12 |
9 |
125151254 |
+ |
PRPS1P2 |
NNNCGACCGCATGGTGCTTG |
NGG |
1 |
0.5294 |
Tier III |
| 18 |
NC_000012.12 |
12 |
4118808 |
+ |
RPL18P9 |
NNNGGACCACATGGTGCTTC |
NGG |
2 |
0.2755 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
140472086 |
+ |
Prps1 |
NNNGGACCGCATGGTACTTG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
34985507 |
+ |
Prps1l1 |
NNNGGACCGCATGGTGCTTG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000069.6 |
3 |
95140556 |
+ |
Tnfaip8l2 |
NNNGGACTCCATGGTGCTTG |
NGG |
2 |
0.4712 |
Tier I |
| 4 |
NC_000075.6 |
9 |
45942230 |
- |
Sidt2 |
NNNGGACCGCATGGTGCTTC |
NGG |
1 |
0.4286 |
Tier I |
| 5 |
NC_000080.6 |
14 |
70604973 |
- |
Dmtn |
NNNGGACCGCATGAGGCTTG |
NGG |
2 |
0.0375 |
Tier I |
| 6 |
NC_000068.7 |
2 |
26392536 |
+ |
Pmpca |
NNNTGACCGCATGGTGCTTG |
NTG |
2 |
0.0142 |
Tier I |
| 7 |
NC_000073.6 |
7 |
35293883 |
+ |
Gpatch1 |
NNNGGAAAGCATGGTGCTTG |
NGG |
2 |
0.4875 |
Tier II |
| 8 |
NC_000068.7 |
2 |
160363956 |
- |
Mafb |
NNNGAACCGCATGGTGCTTG |
NAG |
2 |
0.2247 |
Tier II |
| 9 |
NC_000086.7 |
X |
168155409 |
+ |
Frmpd4 |
NNNGGACCACATGGTGCTTG |
NTG |
2 |
0.025 |
Tier II |
| 10 |
NC_000070.6 |
4 |
136292393 |
- |
Zfp46 |
NNNGGAGCGCATGGTGCTTG |
NGC |
2 |
0.0105 |
Tier II |
| 11 |
NC_000078.6 |
12 |
34985507 |
+ |
Gm33864 |
NNNGGACCGCATGGTGCTTG |
NGG |
0 |
1.0 |
Tier III |
| 12 |
NC_000073.6 |
7 |
69075826 |
- |
Gm34782 |
NNNGGAGCTCATGGTGCTTG |
NGG |
2 |
0.251 |
Tier III |
Other clones with same target sequence:
(none)
