Construct: sgRNA BRDN0001147970

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGTATGTCACGCTCCACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST2 (23139)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76938

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 46023876 - MAST2 NNNTATGTCACGCTCCACGA NGG 0 1.0 Tier I
2 NC_000005.10 5 156167356 + SGCD NNNTATGTCAAGCTCCATGA NGG 2 0.4821 Tier II
3 NC_000001.11 1 238580068 - LOC124904565 NNNGATGTCACGCTCCAAGA NGG 2 0.3365 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 102774291 + Mast4 NNNGATGTCTCGCTCCACGA NGG 2 0.5515 Tier I
2 NC_000073.6 7 25388155 - Lipe NNNTATGTCACGCTACACAA NGG 2 0.1587 Tier I
3 NC_000073.6 7 25388155 - 4732471J01Rik NNNTATGTCACGCTACACAA NGG 2 0.1587 Tier III
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Other clones with same target sequence:

(none)