Construct: sgRNA BRDN0001147973
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATGGGTTGCCGGGAGTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80230
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
108586689 |
+ |
COL4A5 |
NNNGGGTTGCCTGGAGAAAA |
NGG |
2 |
0.2051 |
Tier I |
| 2 |
NC_000011.10 |
11 |
126358344 |
+ |
ST3GAL4 |
NNNTGGTTGCCGGGAGGAAA |
NGG |
2 |
0.0428 |
Tier I |
| 3 |
NC_000023.11 |
X |
107877657 |
- |
MID2 |
NNNAGGTTGCCGGCAGTAAA |
NGG |
2 |
0.3857 |
Tier II |
| 4 |
NC_000004.12 |
4 |
16651240 |
+ |
LDB2 |
NNNTGGTTGCAGGGAGTAAA |
NGG |
2 |
0.2727 |
Tier II |
| 5 |
NC_000019.10 |
19 |
5977871 |
+ |
RANBP3 |
NNNGGGTTGCCGGGAGTGAT |
NGG |
2 |
0.24 |
Tier II |
| 6 |
NC_000002.12 |
2 |
216361016 |
+ |
MARCHF4 |
NNNGGGTTGCAGGGAGTACA |
NGG |
2 |
0.1552 |
Tier II |
| 7 |
NC_000002.12 |
2 |
197910665 |
- |
PLCL1 |
NNNGGGTTGCCGGGAATAAA |
NGA |
2 |
0.0694 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
27968180 |
+ |
Col5a1 |
NNNGGGTTGCCGGGAGAGAA |
NGG |
2 |
0.2133 |
Tier I |
| 2 |
NC_000073.6 |
7 |
82464089 |
- |
Adamtsl3 |
NNNGGGATGCCAGGAGTAAA |
NGG |
2 |
0.8167 |
Tier II |
| 3 |
NC_000067.6 |
1 |
70512534 |
- |
Spag16 |
NNNGGGCTGCAGGGAGTAAA |
NGG |
2 |
0.5156 |
Tier II |
| 4 |
NC_000083.6 |
17 |
71861161 |
- |
Clip4 |
NNNGGGTTGCGGGGGGTAAA |
NGG |
2 |
0.1625 |
Tier II |
| 5 |
NC_000072.6 |
6 |
64420668 |
+ |
Grid2 |
NNNGGGTTGCCCTGAGTAAA |
NGG |
2 |
0.1588 |
Tier II |
| 6 |
NC_000072.6 |
6 |
128091136 |
+ |
Tspan9 |
NNNGGGTTGCTGGGAGTAGA |
NGG |
2 |
0.1154 |
Tier II |
Other clones with same target sequence:
(none)
