Construct: sgRNA BRDN0001147974
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCAGGGGACAAGTAACCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK2G (818)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76239
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000080.6 |
14 |
20765462 |
+ |
Camk2g |
NNNAGGGGACAAGTAACCTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000068.7 |
2 |
119212015 |
- |
Zfyve19 |
NNNAGGGGACAAGTTACCTG |
NGG |
1 |
0.2 |
Tier I |
3 |
NC_000071.6 |
5 |
8956428 |
- |
Abcb4 |
NNNAGGAGACAGGTAACCTG |
NGG |
2 |
0.7222 |
Tier II |
4 |
NC_000075.6 |
9 |
42418857 |
+ |
Tbcel |
NNNAGGGGACAGGTGACCTG |
NGG |
2 |
0.4694 |
Tier II |
5 |
NC_000067.6 |
1 |
93218517 |
- |
Crocc2 |
NNNTGGGGACAAGTGACCTG |
NGG |
2 |
0.4136 |
Tier II |
6 |
NC_000079.6 |
13 |
45707934 |
- |
Atxn1 |
NNNAGGGGACCAGTAACCTA |
NGG |
2 |
0.375 |
Tier II |
7 |
NC_000075.6 |
9 |
35513119 |
- |
Gm1113 |
NNNAGGGGAGAAGTAACCTA |
NGG |
2 |
0.3646 |
Tier II |
8 |
NC_000072.6 |
6 |
145415088 |
+ |
Lmntd1 |
NNNAGGGGACAAGCAACCTT |
NGG |
2 |
0.2 |
Tier II |
9 |
NC_000085.6 |
19 |
53033074 |
- |
Xpnpep1 |
NNNAGGGGACAAGAAACCTG |
NGC |
2 |
0.0138 |
Tier II |
10 |
NC_000082.6 |
16 |
11658813 |
- |
Snx29 |
NNNAGGGGACAAGCAACCTG |
NGT |
2 |
0.0046 |
Tier II |
11 |
NC_000071.6 |
5 |
108702467 |
- |
Fgfrl1 |
NNNAGAGGACAAGTATCCTG |
NGG |
2 |
0.0 |
Tier II |
12 |
NC_000072.6 |
6 |
144337598 |
+ |
Sox5 |
NNNAGGCGACAAGGAACCTG |
NGG |
2 |
0.0 |
Tier II |
13 |
NC_000077.6 |
11 |
113565409 |
- |
Slc39a11 |
NNNAGGGGACAAGGGACCTG |
NGG |
2 |
0.0 |
Tier II |
14 |
NC_000074.6 |
8 |
113832973 |
+ |
Adamts18 |
NNNAGGGGACAAGTAACCTG |
NCA |
2 |
0.0 |
Tier II |
15 |
NC_000081.6 |
15 |
39118078 |
- |
Dcaf13 |
NNNAGGGGACAAGTAACCTG |
NCT |
2 |
0.0 |
Tier II |
16 |
NC_000068.7 |
2 |
164834529 |
+ |
Ctsa |
NNNAGGGGAGAAGGAACCTG |
NGG |
2 |
0.0 |
Tier II |
17 |
NC_000069.6 |
3 |
35546348 |
+ |
Ube2l3-ps1 |
NNNAGGGGAAAAGTGACCTG |
NGG |
2 |
0.5633 |
Tier III |
18 |
NC_000079.6 |
13 |
41538833 |
- |
Gm32184 |
NNNAAGGGACAAGAAACCTG |
NGG |
2 |
0.5365 |
Tier III |
19 |
NC_000072.6 |
6 |
69342491 |
+ |
Igk |
NNNAGGGAACAAGTAACCTG |
NTG |
2 |
0.039 |
Tier III |
20 |
NC_000072.6 |
6 |
69342491 |
+ |
Igkv4-65 |
NNNAGGGAACAAGTAACCTG |
NTG |
2 |
0.039 |
Tier III |
21 |
NC_000068.7 |
2 |
145712388 |
+ |
BC039771 |
NNNAGGGGACAAGTCACCTG |
NGT |
2 |
0.0044 |
Tier III |
22 |
NC_000068.7 |
2 |
145712388 |
+ |
Gm34513 |
NNNAGGGGACAAGTCACCTG |
NGT |
2 |
0.0044 |
Tier III |
23 |
NC_000079.6 |
13 |
59207111 |
+ |
Gm34558 |
NNNAGGGGACAAGTACCCTG |
NGA |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)