Construct: sgRNA BRDN0001147974

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCAGGGGACAAGTAACCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2G (818)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76239

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 73848592 + CAMK2G NNNAGGGGACAAGTAACCTG NGG 0 1.0 Tier I
2 NC_000011.10 11 61779931 - MYRF NNNAGGGGACAAGTCACCTG NAG 2 0.0707 Tier I
3 NC_000012.12 12 68900658 + CPM NNNAGGGGATAAATAACCTG NGG 2 0.8688 Tier II
4 NC_000006.12 6 126095271 + TRMT11 NNNAGGAGACAGGTAACCTG NGG 2 0.7222 Tier II
5 NC_000002.12 2 124290633 - CNTNAP5 NNNAGAGGTCAAGTAACCTG NGG 2 0.6 Tier II
6 NC_000002.12 2 214059725 - SPAG16 NNNAGGGTATAAGTAACCTG NGG 2 0.5882 Tier II
7 NC_000004.12 4 78169927 - FRAS1 NNNAGGGAACAAGTAAACTG NGG 2 0.4667 Tier II
8 NC_000010.11 10 99907868 + DNMBP NNNAGAGGACAAGTAACCTC NGG 2 0.4286 Tier II
9 NC_000016.10 16 57020531 - NLRC5 NNNAGGGGACAGGTAAACTG NGG 2 0.337 Tier II
10 NC_000010.11 10 16612907 - RSU1 NNNAGGGGACAAATAACCAG NGG 2 0.2637 Tier II
11 NC_000017.11 17 42519427 - ATP6V0A1 NNNAGTGGACAAGTCACCTG NGG 2 0.1818 Tier II
12 NC_000020.11 20 4157563 + SMOX NNNAGGGGACAAGTGGCCTG NGG 2 0.125 Tier II
13 NC_000017.11 17 48916017 - UBE2Z NNNAGGGAACAAGTACCCTG NGG 2 0.0 Tier II
14 NC_000017.11 17 5016119 + KIF1C NNNAGGGGACAAGGGACCTG NGG 2 0.0 Tier II
15 NC_000019.10 19 54140644 + CNOT3 NNNAGGGGACAAGTACCCTG NGC 2 0.0 Tier II
16 NC_000001.11 1 203041678 + PPFIA4 NNNAGGGGACAGGTATCCTG NGG 2 0.0 Tier II
17 NC_000005.10 5 4775731 - LOC107986400 NNNAGGGGAAAAGTAACCTA NGG 2 0.8125 Tier III
18 NC_000017.11 17 53973267 + LOC107985001 NNNAGGGGTGAAGTAACCTG NGG 2 0.2333 Tier III
19 NC_000003.12 3 67701214 - SUCLG2-DT NNNAAGGGACAAGTAACCTG NAG 2 0.2247 Tier III
20 NC_000008.11 8 144705738 - LOC100996662 NNNAGGGGACCAGTCACCTG NGG 2 0.1091 Tier III
21 NC_000003.12 3 706051 + LINC01266 NNNAGGGGAAAAGTAAGCTG NGG 2 0.051 Tier III
22 NC_000023.11 X 129064794 - LOC124905213 NNNAAGGGACAAGGAACCTG NGG 2 0.0 Tier III
23 NC_000001.11 1 69541255 - LOC105378789 NNNAGGGGAAAAGGAACCTG NGG 2 0.0 Tier III
24 NC_000009.12 9 16253647 + LINC03041 NNNAGGGGAGAAGGAACCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 20765462 + Camk2g NNNAGGGGACAAGTAACCTG NGG 0 1.0 Tier I
2 NC_000068.7 2 119212015 - Zfyve19 NNNAGGGGACAAGTTACCTG NGG 1 0.2 Tier I
3 NC_000071.6 5 8956428 - Abcb4 NNNAGGAGACAGGTAACCTG NGG 2 0.7222 Tier II
4 NC_000075.6 9 42418857 + Tbcel NNNAGGGGACAGGTGACCTG NGG 2 0.4694 Tier II
5 NC_000067.6 1 93218517 - Crocc2 NNNTGGGGACAAGTGACCTG NGG 2 0.4136 Tier II
6 NC_000079.6 13 45707934 - Atxn1 NNNAGGGGACCAGTAACCTA NGG 2 0.375 Tier II
7 NC_000075.6 9 35513119 - Gm1113 NNNAGGGGAGAAGTAACCTA NGG 2 0.3646 Tier II
8 NC_000072.6 6 145415088 + Lmntd1 NNNAGGGGACAAGCAACCTT NGG 2 0.2 Tier II
9 NC_000085.6 19 53033074 - Xpnpep1 NNNAGGGGACAAGAAACCTG NGC 2 0.0138 Tier II
10 NC_000082.6 16 11658813 - Snx29 NNNAGGGGACAAGCAACCTG NGT 2 0.0046 Tier II
11 NC_000071.6 5 108702467 - Fgfrl1 NNNAGAGGACAAGTATCCTG NGG 2 0.0 Tier II
12 NC_000072.6 6 144337598 + Sox5 NNNAGGCGACAAGGAACCTG NGG 2 0.0 Tier II
13 NC_000077.6 11 113565409 - Slc39a11 NNNAGGGGACAAGGGACCTG NGG 2 0.0 Tier II
14 NC_000074.6 8 113832973 + Adamts18 NNNAGGGGACAAGTAACCTG NCA 2 0.0 Tier II
15 NC_000081.6 15 39118078 - Dcaf13 NNNAGGGGACAAGTAACCTG NCT 2 0.0 Tier II
16 NC_000068.7 2 164834529 + Ctsa NNNAGGGGAGAAGGAACCTG NGG 2 0.0 Tier II
17 NC_000069.6 3 35546348 + Ube2l3-ps1 NNNAGGGGAAAAGTGACCTG NGG 2 0.5633 Tier III
18 NC_000079.6 13 41538833 - Gm32184 NNNAAGGGACAAGAAACCTG NGG 2 0.5365 Tier III
19 NC_000072.6 6 69342491 + Igk NNNAGGGAACAAGTAACCTG NTG 2 0.039 Tier III
20 NC_000072.6 6 69342491 + Igkv4-65 NNNAGGGAACAAGTAACCTG NTG 2 0.039 Tier III
21 NC_000068.7 2 145712388 + BC039771 NNNAGGGGACAAGTCACCTG NGT 2 0.0044 Tier III
22 NC_000068.7 2 145712388 + Gm34513 NNNAGGGGACAAGTCACCTG NGT 2 0.0044 Tier III
23 NC_000079.6 13 59207111 + Gm34558 NNNAGGGGACAAGTACCCTG NGA 2 0.0 Tier III
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Other clones with same target sequence:

(none)