Construct: sgRNA BRDN0001147976

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAATCAGACTCATGCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK8 (5599)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78049

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 48404931 + MAPK8 NNNATCAGACTCATGCCAAG NGG 0 1.0 Tier I
2 NC_000008.11 8 6812166 - XKR5 NNNCTCAGGCTCATGCCAAG NGG 2 0.2353 Tier I
3 NC_000014.9 14 74834622 + YLPM1 NNNACTAGACTCATGCCAAG NGG 2 0.9286 Tier II
4 NC_000006.12 6 35014471 - ANKS1A NNNACCAGACACATGCCAAG NGG 2 0.75 Tier II
5 NC_000001.11 1 208116251 - PLXNA2 NNNATTAGACTCATGCCAAT NGG 2 0.65 Tier II
6 NC_000019.10 19 12537073 + ZNF564 NNNATCAGAATCATGCCATG NGG 2 0.4667 Tier II
7 NC_000008.11 8 118209550 - SAMD12 NNNTTGAGACTCATGCCAAG NGG 2 0.3182 Tier II
8 NC_000001.11 1 44348387 + ERI3 NNNATCAGGCTCATGCAAAG NGG 2 0.3111 Tier II
9 NC_000009.12 9 112411249 + HSDL2 NNNATGAGACTTATGCCAAG NGG 2 0.2692 Tier II
10 NC_000005.10 5 133304935 + FSTL4 NNNATCAGACACTTGCCAAG NGG 2 0.225 Tier II
11 NC_000023.11 X 103824776 - RAB9B NNNATCAGACTCTTGCCAAG NAG 2 0.0778 Tier II
12 NC_000002.12 2 17753249 + SMC6 NNNATCAGACTCATGCTAAG NGC 2 0.0104 Tier II
13 NC_000006.12 6 116114988 + NT5DC1 NNNATCAGACGCAGGCCAAG NGG 2 0.0 Tier II
14 NC_000011.10 11 91869324 - LINC02756 NNNTTCAGACTCATGCCAAG NAG 2 0.165 Tier III
15 NC_000002.12 2 113364637 + LINC02966 NNNATGAGACTCATGCCCAG NGG 2 0.0952 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 34799534 - Htt NNNACAAGACTCATGCCAAG NGG 2 0.9286 Tier II
2 NC_000084.6 18 23858310 - Mapre2 NNNATTAGACTCATGACAAG NGG 2 0.9286 Tier II
3 NC_000082.6 16 40827145 + Lsamp NNNATCAGACTCGTGCCAAA NGG 2 0.6114 Tier II
4 NC_000085.6 19 30617238 - Prkg1 NNNATCAGACTTATGCCAAA NGG 2 0.5048 Tier II
5 NC_000068.7 2 21765801 - Gpr158 NNNTTCAGACTCATGCCAAT NGG 2 0.4455 Tier II
6 NC_000078.6 12 103545926 - Ppp4r4 NNNATCAGAGTCATACCAAG NGG 2 0.366 Tier II
7 NC_000070.6 4 123921398 - Rragc NNNAGCAGACTCATGCCATG NGG 2 0.3446 Tier II
8 NC_000085.6 19 20928905 - Tmc1 NNNATCAGACTCCTGCCAAG NGG 1 0.2105 Tier II
9 NC_000074.6 8 116718948 + Cdyl2 NNNATCAGCCTCATGCCAAG NAG 2 0.1481 Tier II
10 NC_000068.7 2 13373490 - Cubn NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
11 NC_000068.7 2 18087070 + Mllt10 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
12 NC_000068.7 2 48967554 + Mbd5 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
13 NC_000069.6 3 108680486 - Gpsm2 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
14 NC_000069.6 3 153902853 - Msh4 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
15 NC_000070.6 4 11577952 + Rad54b NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
16 NC_000071.6 5 14067769 + Sema3e NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
17 NC_000071.6 5 114169662 + Acacb NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
18 NC_000073.6 7 29214385 - Catsperg1 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
19 NC_000074.6 8 68456713 - Csgalnact1 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
20 NC_000074.6 8 79720433 + Anapc10 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
21 NC_000075.6 9 122876155 - Zfp445 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
22 NC_000076.6 10 33353426 + Trdn NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
23 NC_000079.6 13 45522523 + Gmpr NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
24 NC_000079.6 13 49102230 - Wnk2 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
25 NC_000084.6 18 5082304 + Svil NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
26 NC_000085.6 19 7452862 - Rtn3 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
27 NC_000086.7 X 147478925 - Lrch2 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier II
28 NC_000075.6 9 43742306 - Nectin1 NNNATCAGACTCATGCCAGG NAG 2 0.0972 Tier II
29 NC_000073.6 7 120893042 + Eef2k NNNCTCAGACTCATGCCCAG NGG 2 0.0672 Tier II
30 NC_000085.6 19 57923578 - Atrnl1 NNNATCAGACTCATGGCAAG NGT 2 0.0025 Tier II
31 NC_000083.6 17 88278161 - Gm31615 NNNGTCAGATTCATGCCAAG NGG 2 0.5882 Tier III
32 NC_000068.7 2 74581933 - 4930445N08Rik NNNATCAGATTCCTGCCAAG NGG 2 0.1981 Tier III
33 NC_000077.6 11 29642232 - Gm12092 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier III
34 NC_000079.6 13 45522523 + Gm34582 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier III
35 NC_000079.6 13 92897836 - Gm31630 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier III
36 NC_000080.6 14 120852837 + Gm52124 NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier III
37 NC_000085.6 19 38993542 + Cyp2c52-ps NNNGTCAGACTCCTGCCAAG NGG 2 0.1316 Tier III
38 NC_000075.6 9 43742306 - Gm46108 NNNATCAGACTCATGCCAGG NAG 2 0.0972 Tier III
39 NC_000070.6 4 55304646 - Gm52739 NNNATCTGACTCATGCCCAG NGG 2 0.0833 Tier III
40 NC_000079.6 13 114464980 - Gm41074 NNNATAAGACTCATGCCAAG NGT 2 0.015 Tier III
41 NC_000085.6 19 57923578 - Gm52385 NNNATCAGACTCATGGCAAG NGT 2 0.0025 Tier III
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Other clones with same target sequence:

(none)