Construct: sgRNA BRDN0001147981
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCGAGTAATCATCCCACTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKG1 (5260)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76357
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
56081926 |
+ |
PHKG1 |
NNNGAGTAATCATCCCACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
56081926 |
+ |
SUMF2 |
NNNGAGTAATCATCCCACTC |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000021.9 |
21 |
21230118 |
- |
NCAM2 |
NNNGAGTAATCATCCAAATC |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000007.14 |
7 |
93571255 |
- |
CALCR |
NNNAAGTAATCATCCCAATC |
NGG |
2 |
0.4846 |
Tier II |
| 5 |
NC_000001.11 |
1 |
37549518 |
+ |
SNIP1 |
NNNAAGTAATCCTCCCACTC |
NGG |
2 |
0.2368 |
Tier II |
| 6 |
NC_000003.12 |
3 |
113651456 |
+ |
USF3 |
NNNGAGTAATCATCCCGCTA |
NGG |
2 |
0.0882 |
Tier II |
| 7 |
NC_000003.12 |
3 |
131711535 |
+ |
CPNE4 |
NNNGAGTAATCAGCCCACCC |
NGG |
2 |
0.072 |
Tier II |
| 8 |
NC_000002.12 |
2 |
140735301 |
- |
LRP1B |
NNNCAGTAATCATCCCACTC |
NGC |
2 |
0.0118 |
Tier II |
| 9 |
NC_000011.10 |
11 |
50247433 |
+ |
PHKG1P3 |
NNNAAGTAATCGTCCCACTC |
NGG |
2 |
0.65 |
Tier III |
| 10 |
NC_000007.14 |
7 |
63233922 |
+ |
PHKG1P1 |
NNNGAGTAATCATCCTACTC |
NGG |
1 |
0.3077 |
Tier III |
| 11 |
NC_000020.11 |
20 |
43762552 |
+ |
LOC101927200 |
NNNAAGTAATCTTCCCACTC |
NGG |
2 |
0.3 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
129865950 |
+ |
Phkg1 |
NNNGAGTAGTCATCCCACTC |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000081.6 |
15 |
94654714 |
+ |
Tmem117 |
NNNAAGTAATAATCCCACTC |
NGG |
2 |
0.675 |
Tier II |
| 3 |
NC_000078.6 |
12 |
84400312 |
+ |
Entpd5 |
NNNGATTAATCACCCCACTC |
NGG |
2 |
0.5263 |
Tier II |
| 4 |
NC_000076.6 |
10 |
77741215 |
+ |
Tspear |
NNNGAGTAATCCTCACACTC |
NGG |
2 |
0.0585 |
Tier II |
| 5 |
NC_000068.7 |
2 |
67679689 |
+ |
B3galt1 |
NNNGAGTAATCACCCCACTC |
NTG |
2 |
0.0308 |
Tier II |
| 6 |
NC_000076.6 |
10 |
69149432 |
+ |
Gm33263 |
NNNGAGTCATCTTCCCACTC |
NGG |
2 |
0.1429 |
Tier III |
Other clones with same target sequence:
(none)
