Construct: sgRNA BRDN0001147988
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGACTTCGGAACATCAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K5 (4217)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76656
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
136611325 |
- |
MAP3K5 |
NNNACTTCGGAACATCAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
99630055 |
- |
MEF2A |
NNNACTTAGGAACATCAAAG |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000009.12 |
9 |
117065473 |
- |
ASTN2 |
NNNACTTAAGAACATCAAAG |
NGG |
2 |
0.4179 |
Tier II |
| 4 |
NC_000001.11 |
1 |
173505483 |
- |
SLC9C2 |
NNNACTTCTCAACATCAAAG |
NGG |
2 |
0.2133 |
Tier II |
| 5 |
NC_000011.10 |
11 |
11459020 |
+ |
GALNT18 |
NNNACTTCGGCACATCATAG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000006.12 |
6 |
56550585 |
+ |
DST |
NNNACTTGGGAACACCAAAG |
NGG |
2 |
0.1753 |
Tier II |
| 7 |
NC_000003.12 |
3 |
13493883 |
- |
HDAC11 |
NNNACTTGGGAACCTCAAAG |
NGG |
2 |
0.1378 |
Tier II |
| 8 |
NC_000004.12 |
4 |
168910025 |
- |
PALLD |
NNNACTTCTGAACATGAAAG |
NGG |
2 |
0.0821 |
Tier II |
| 9 |
NC_000004.12 |
4 |
168910025 |
- |
CBR4 |
NNNACTTCTGAACATGAAAG |
NGG |
2 |
0.0821 |
Tier II |
| 10 |
NC_000010.11 |
10 |
85462710 |
+ |
LINC01520 |
NNNACTTCAGAACATCATAG |
NGG |
2 |
0.3214 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
20104223 |
+ |
Map3k5 |
NNNACTTCGGGACATCCAAG |
NGG |
2 |
0.1147 |
Tier I |
| 2 |
NC_000069.6 |
3 |
34022425 |
+ |
Fxr1 |
NNNACTTTGGAACATCAAAA |
NGG |
2 |
0.8203 |
Tier II |
| 3 |
NC_000079.6 |
13 |
96941601 |
+ |
Gcnt4 |
NNNGCTTCGGAACACCAAAG |
NGG |
2 |
0.1705 |
Tier II |
| 4 |
NC_000071.6 |
5 |
113797732 |
+ |
Tmem119 |
NNNACTTCAGAACATCAAAG |
NAG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000069.6 |
3 |
25953279 |
+ |
Nlgn1 |
NNNACTTCTGAACATCAAAG |
NAG |
2 |
0.1383 |
Tier II |
| 6 |
NC_000076.6 |
10 |
21899021 |
- |
Sgk1 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 7 |
NC_000077.6 |
11 |
43053225 |
+ |
Atp10b |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 8 |
NC_000079.6 |
13 |
76450342 |
+ |
Mctp1 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 9 |
NC_000079.6 |
13 |
113314201 |
+ |
Cspg4b |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 10 |
NC_000081.6 |
15 |
44210177 |
+ |
Trhr |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 11 |
NC_000082.6 |
16 |
28668241 |
- |
Fgf12 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 12 |
NC_000082.6 |
16 |
31003801 |
- |
Xxylt1 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 13 |
NC_000086.7 |
X |
7249431 |
- |
Clcn5 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 14 |
NC_000086.7 |
X |
78514689 |
- |
Prrg1 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 15 |
NC_000086.7 |
X |
139571117 |
+ |
Rnf128 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier II |
| 16 |
NC_000082.6 |
16 |
26279604 |
+ |
Gm41436 |
NNNACATCGGAACATCAAAG |
NGG |
1 |
0.8667 |
Tier III |
| 17 |
NC_000069.6 |
3 |
45781840 |
+ |
Gm32340 |
NNNACTTCGGAACAGCAAAG |
NGG |
1 |
0.05 |
Tier III |
| 18 |
NC_000068.7 |
2 |
30164871 |
- |
Gm31080 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 19 |
NC_000069.6 |
3 |
27280344 |
+ |
Gm42192 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 20 |
NC_000073.6 |
7 |
80977233 |
+ |
Gm32178 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 21 |
NC_000076.6 |
10 |
15727712 |
+ |
Gm40604 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 22 |
NC_000079.6 |
13 |
50452478 |
- |
Gm40946 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 23 |
NC_000080.6 |
14 |
123211586 |
+ |
Gm33906 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 24 |
NC_000081.6 |
15 |
38145674 |
+ |
Gm35081 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 25 |
NC_000082.6 |
16 |
95764257 |
+ |
Gm32130 |
NNNGCTTCGGAACAGCAAAG |
NGG |
2 |
0.0313 |
Tier III |
| 26 |
NC_000075.6 |
9 |
8697352 |
- |
Gm32014 |
NNNACTTCAGAACATCAAAG |
NTG |
2 |
0.025 |
Tier III |
Other clones with same target sequence:
(none)
