Construct: sgRNA BRDN0001147989
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCAGCCAAGCAGACGACCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ARSG (22901)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77640
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
68385088 |
+ |
ARSG |
NNNAGCCAAGCAGACGACCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
58564516 |
- |
FAM107A |
NNNAGCCGTGCAGACGACCT |
NGG |
2 |
0.44 |
Tier II |
| 3 |
NC_000018.10 |
18 |
70014871 |
+ |
RTTN |
NNNAGCCAAGCATACAACCT |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000001.11 |
1 |
150454086 |
- |
RPRD2 |
NNNAGCCAAGCAGAAAACCT |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000018.10 |
18 |
59496771 |
+ |
CCBE1 |
NNNAGCTAAGCAGCCGACCT |
NGG |
2 |
0.1741 |
Tier II |
| 6 |
NC_000023.11 |
X |
47204691 |
+ |
UBA1 |
NNNAGCCAAGGAGCCGACCT |
NGG |
2 |
0.0536 |
Tier II |
| 7 |
NC_000011.10 |
11 |
61824690 |
- |
FADS2 |
NNNAGCCAAGCAGCAGACCT |
NGG |
2 |
0.0476 |
Tier II |
| 8 |
NC_000007.14 |
7 |
112073080 |
- |
DOCK4 |
NNNGGCCAAGCAGACGACCT |
NGA |
2 |
0.0434 |
Tier II |
| 9 |
NC_000023.11 |
X |
77556758 |
- |
ATRX |
NNNAGCAAAGCAGAGGACCT |
NGG |
2 |
0.0375 |
Tier II |
| 10 |
NC_000010.11 |
10 |
69506088 |
- |
TSPAN15 |
NNNAGCCCAGCAGAGGACCT |
NGG |
2 |
0.0214 |
Tier II |
| 11 |
NC_000011.10 |
11 |
44918857 |
- |
TSPAN18 |
NNNAGCCAAGCAGATGCCCT |
NGG |
2 |
0.0118 |
Tier II |
| 12 |
NC_000003.12 |
3 |
58591350 |
+ |
FAM107A |
NNNAGCCAAGCAGATGTCCT |
NGG |
2 |
0.0089 |
Tier II |
| 13 |
NC_000010.11 |
10 |
112192060 |
- |
GPAM |
NNNAGCCAAGCAGAGGCCCT |
NGG |
2 |
0.0088 |
Tier II |
| 14 |
NC_000012.12 |
12 |
29192392 |
+ |
FAR2 |
NNNAGCCAAGAAGACTACCT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000003.12 |
3 |
58564516 |
- |
LOC107984079 |
NNNAGCCGTGCAGACGACCT |
NGG |
2 |
0.44 |
Tier III |
| 16 |
NC_000023.11 |
X |
47204691 |
+ |
LOC105373194 |
NNNAGCCAAGGAGCCGACCT |
NGG |
2 |
0.0536 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
89161073 |
+ |
Plxnb2 |
NNNAGACAAGAAGACGACCT |
NGG |
2 |
0.6964 |
Tier I |
| 2 |
NC_000077.6 |
11 |
109548038 |
+ |
Arsg |
NNNAACCAAGCAGACCACCT |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000067.6 |
1 |
161981310 |
- |
4930558K02Rik |
NNNAGCCATGCAGACAACCT |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000076.6 |
10 |
130362738 |
+ |
Tespa1 |
NNNATCCAAGCAAACGACCT |
NGG |
2 |
0.2769 |
Tier II |
| 5 |
NC_000073.6 |
7 |
120448535 |
- |
Abca16 |
NNNAGGGAAGCAGACGACCT |
NGG |
2 |
0.2353 |
Tier II |
| 6 |
NC_000082.6 |
16 |
43540990 |
+ |
Zbtb20 |
NNNTGCCAAGGAGACGACCT |
NGG |
2 |
0.1591 |
Tier II |
| 7 |
NC_000073.6 |
7 |
124232903 |
+ |
Hs3st4 |
NNNAGCCAGGCAGAAGACCT |
NGG |
2 |
0.1481 |
Tier II |
| 8 |
NC_000085.6 |
19 |
43982846 |
+ |
Cpn1 |
NNNAGCCAAGTAGACGACCT |
NAG |
2 |
0.0798 |
Tier II |
| 9 |
NC_000085.6 |
19 |
44534540 |
- |
Sec31b |
NNNAGCCAAGCAGATGACCT |
NGG |
1 |
0.0667 |
Tier II |
| 10 |
NC_000071.6 |
5 |
71616956 |
+ |
Gabra4 |
NNNAGCCAAGCAGACGAGCT |
NAG |
2 |
0.0346 |
Tier II |
| 11 |
NC_000069.6 |
3 |
105763784 |
+ |
Rap1a |
NNNAGCCAAGCAGAGGATCT |
NGG |
2 |
0.0321 |
Tier II |
| 12 |
NC_000067.6 |
1 |
124621041 |
+ |
Dpp10 |
NNNAGCCATGCAGAGGACCT |
NGG |
2 |
0.03 |
Tier II |
| 13 |
NC_000070.6 |
4 |
59909368 |
- |
Slc46a2 |
NNNAGCCAAGCAGAGGACCA |
NGG |
2 |
0.0281 |
Tier II |
| 14 |
NC_000074.6 |
8 |
109378976 |
+ |
Pmfbp1 |
NNNAGCCAAGCAGATGTCCT |
NGG |
2 |
0.0089 |
Tier II |
| 15 |
NC_000068.7 |
2 |
153803202 |
- |
Efcab8 |
NNNAGCCAAGCAGAAGACCT |
NTG |
2 |
0.0087 |
Tier II |
| 16 |
NC_000073.6 |
7 |
81380392 |
+ |
Cpeb1 |
NNNAGCCAAGCAGAGGTCCT |
NGG |
2 |
0.0067 |
Tier II |
| 17 |
NC_000082.6 |
16 |
6186763 |
- |
Rbfox1 |
NNNAACCAAGCAGACCACCT |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000084.6 |
18 |
43248998 |
- |
Stk32a |
NNNAGCCAAGCAAACTACCT |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000069.6 |
3 |
98680874 |
- |
Gm12400 |
NNNAGCCAAGCAGAAAACCT |
NGG |
2 |
0.2222 |
Tier III |
| 20 |
NC_000070.6 |
4 |
129396423 |
+ |
C330020E22Rik |
NNNAGCCAAGCAGACGACCA |
NGT |
2 |
0.0091 |
Tier III |
| 21 |
NC_000084.6 |
18 |
43248998 |
- |
Gm31647 |
NNNAGCCAAGCAAACTACCT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
