Construct: sgRNA BRDN0001147989

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCAGCCAAGCAGACGACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARSG (22901)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77640

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 68385088 + ARSG NNNAGCCAAGCAGACGACCT NGG 0 1.0 Tier I
2 NC_000003.12 3 58564516 - FAM107A NNNAGCCGTGCAGACGACCT NGG 2 0.44 Tier II
3 NC_000018.10 18 70014871 + RTTN NNNAGCCAAGCATACAACCT NGG 2 0.3 Tier II
4 NC_000001.11 1 150454086 - RPRD2 NNNAGCCAAGCAGAAAACCT NGG 2 0.2222 Tier II
5 NC_000018.10 18 59496771 + CCBE1 NNNAGCTAAGCAGCCGACCT NGG 2 0.1741 Tier II
6 NC_000023.11 X 47204691 + UBA1 NNNAGCCAAGGAGCCGACCT NGG 2 0.0536 Tier II
7 NC_000011.10 11 61824690 - FADS2 NNNAGCCAAGCAGCAGACCT NGG 2 0.0476 Tier II
8 NC_000007.14 7 112073080 - DOCK4 NNNGGCCAAGCAGACGACCT NGA 2 0.0434 Tier II
9 NC_000023.11 X 77556758 - ATRX NNNAGCAAAGCAGAGGACCT NGG 2 0.0375 Tier II
10 NC_000010.11 10 69506088 - TSPAN15 NNNAGCCCAGCAGAGGACCT NGG 2 0.0214 Tier II
11 NC_000011.10 11 44918857 - TSPAN18 NNNAGCCAAGCAGATGCCCT NGG 2 0.0118 Tier II
12 NC_000003.12 3 58591350 + FAM107A NNNAGCCAAGCAGATGTCCT NGG 2 0.0089 Tier II
13 NC_000010.11 10 112192060 - GPAM NNNAGCCAAGCAGAGGCCCT NGG 2 0.0088 Tier II
14 NC_000012.12 12 29192392 + FAR2 NNNAGCCAAGAAGACTACCT NGG 2 0.0 Tier II
15 NC_000003.12 3 58564516 - LOC107984079 NNNAGCCGTGCAGACGACCT NGG 2 0.44 Tier III
16 NC_000023.11 X 47204691 + LOC105373194 NNNAGCCAAGGAGCCGACCT NGG 2 0.0536 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 89161073 + Plxnb2 NNNAGACAAGAAGACGACCT NGG 2 0.6964 Tier I
2 NC_000077.6 11 109548038 + Arsg NNNAACCAAGCAGACCACCT NGG 2 0.0 Tier I
3 NC_000067.6 1 161981310 - 4930558K02Rik NNNAGCCATGCAGACAACCT NGG 2 0.6 Tier II
4 NC_000076.6 10 130362738 + Tespa1 NNNATCCAAGCAAACGACCT NGG 2 0.2769 Tier II
5 NC_000073.6 7 120448535 - Abca16 NNNAGGGAAGCAGACGACCT NGG 2 0.2353 Tier II
6 NC_000082.6 16 43540990 + Zbtb20 NNNTGCCAAGGAGACGACCT NGG 2 0.1591 Tier II
7 NC_000073.6 7 124232903 + Hs3st4 NNNAGCCAGGCAGAAGACCT NGG 2 0.1481 Tier II
8 NC_000085.6 19 43982846 + Cpn1 NNNAGCCAAGTAGACGACCT NAG 2 0.0798 Tier II
9 NC_000085.6 19 44534540 - Sec31b NNNAGCCAAGCAGATGACCT NGG 1 0.0667 Tier II
10 NC_000071.6 5 71616956 + Gabra4 NNNAGCCAAGCAGACGAGCT NAG 2 0.0346 Tier II
11 NC_000069.6 3 105763784 + Rap1a NNNAGCCAAGCAGAGGATCT NGG 2 0.0321 Tier II
12 NC_000067.6 1 124621041 + Dpp10 NNNAGCCATGCAGAGGACCT NGG 2 0.03 Tier II
13 NC_000070.6 4 59909368 - Slc46a2 NNNAGCCAAGCAGAGGACCA NGG 2 0.0281 Tier II
14 NC_000074.6 8 109378976 + Pmfbp1 NNNAGCCAAGCAGATGTCCT NGG 2 0.0089 Tier II
15 NC_000068.7 2 153803202 - Efcab8 NNNAGCCAAGCAGAAGACCT NTG 2 0.0087 Tier II
16 NC_000073.6 7 81380392 + Cpeb1 NNNAGCCAAGCAGAGGTCCT NGG 2 0.0067 Tier II
17 NC_000082.6 16 6186763 - Rbfox1 NNNAACCAAGCAGACCACCT NGG 2 0.0 Tier II
18 NC_000084.6 18 43248998 - Stk32a NNNAGCCAAGCAAACTACCT NGG 2 0.0 Tier II
19 NC_000069.6 3 98680874 - Gm12400 NNNAGCCAAGCAGAAAACCT NGG 2 0.2222 Tier III
20 NC_000070.6 4 129396423 + C330020E22Rik NNNAGCCAAGCAGACGACCA NGT 2 0.0091 Tier III
21 NC_000084.6 18 43248998 - Gm31647 NNNAGCCAAGCAAACTACCT NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)

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