Construct: sgRNA BRDN0001148000

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACATAACAGGGATTTGAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2A (5305)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76576

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 22609698 + PIP4K2A NNNTAACAGGGATTTGAACA NGG 0 1.0 Tier I
2 NC_000011.10 11 111356921 + POU2AF1 NNNGGACAGGGATTTGAACA NGG 2 0.45 Tier II
3 NC_000007.14 7 2915468 - CARD11 NNNGAGCAGGGATTTGAACA NGG 2 0.4464 Tier II
4 NC_000011.10 11 124957150 + CCDC15 NNNTAACAGGAATTTGAGCA NGG 2 0.4 Tier II
5 NC_000001.11 1 100995645 + DPH5 NNNTAACAGGGATTTGAGCA NGG 1 0.4 Tier II
6 NC_000001.11 1 100995645 + SLC30A7 NNNTAACAGGGATTTGAGCA NGG 1 0.4 Tier II
7 NC_000005.10 5 160733724 + ATP10B NNNTAACAGTGATTTGAATA NGG 2 0.375 Tier II
8 NC_000001.11 1 237753498 + RYR2 NNNTACCAGTGATTTGAACA NGG 2 0.3693 Tier II
9 NC_000001.11 1 7496356 + CAMTA1 NNNAGACAGGGATTTGAACA NGG 2 0.3429 Tier II
10 NC_000002.12 2 223814551 + AP1S3 NNNAATCAGGGATTTGAACA NGG 2 0.3401 Tier II
11 NC_000007.14 7 8568917 + NXPH1 NNNCAACAGGGATTTGATCA NGG 2 0.3235 Tier II
12 NC_000001.11 1 84309552 - SAMD13 NNNTAACAGAGATCTGAACA NGG 2 0.2667 Tier II
13 NC_000005.10 5 167518217 - TENM2 NNNGTACAGGGATTTGAACA NGG 2 0.2273 Tier II
14 NC_000011.10 11 94802233 - AMOTL1 NNNTACCAGGGATTTGATCA NGG 2 0.2273 Tier II
15 NC_000016.10 16 49656950 + ZNF423 NNNTAGCAGGGCTTTGAACA NGG 2 0.188 Tier II
16 NC_000003.12 3 94019179 - ARL13B NNNTAACAGGGACTTGAACC NGG 2 0.1794 Tier II
17 NC_000003.12 3 94019179 - STX19 NNNTAACAGGGACTTGAACC NGG 2 0.1794 Tier II
18 NC_000001.11 1 59574505 + FGGY NNNTTCCAGGGATTTGAACA NGG 2 0.1653 Tier II
19 NC_000007.14 7 50121998 + SPMIP7 NNNTTCCAGGGATTTGAACA NGG 2 0.1653 Tier II
20 NC_000006.12 6 132683684 + VNN1 NNNTAACAGGGATTTGAACT NAG 2 0.1556 Tier II
21 NC_000005.10 5 116531035 + SEMA6A NNNGAACAGGGATTTGAACC NGG 2 0.142 Tier II
22 NC_000005.10 5 178437031 + COL23A1 NNNGAACAGGGATTTGAACC NGG 2 0.142 Tier II
23 NC_000010.11 10 99815164 - ABCC2 NNNTAACTGGGAGTTGAACA NGG 2 0.1118 Tier II
24 NC_000011.10 11 4017967 - STIM1 NNNTTACAGGGATTTGAACA NAG 2 0.0943 Tier II
25 NC_000004.12 4 98270697 + RAP1GDS1 NNNTAACATGGATTTGAACA NCG 2 0.0571 Tier II
26 NC_000005.10 5 177071867 - ZNF346 NNNTAACAGGGCTTTGCACA NGG 2 0.0464 Tier II
27 NC_000001.11 1 15722443 - PLEKHM2 NNNTAACAGGGATTTGGACC NGG 2 0.0401 Tier II
28 NC_000001.11 1 117518330 - MAN1A2 NNNTAAAAGGGATTTGAACA NTG 2 0.0292 Tier II
29 NC_000003.12 3 140016430 + CLSTN2 NNNTAACATGGATTTGAACA NTG 2 0.0208 Tier II
30 NC_000007.14 7 107435827 + COG5 NNNTAACAGGGATTTGAATA NGT 2 0.0074 Tier II
31 NC_000006.12 6 3372326 + SLC22A23 NNNTAACAGGGATTTGAAAA NGT 2 0.0069 Tier II
32 NC_000005.10 5 137155854 - SPOCK1 NNNTAACAGGGCTTTGAACA NGC 2 0.0058 Tier II
33 NC_000012.12 12 44004233 + TMEM117 NNNTAACAGGGATTATAACA NGG 2 0.0 Tier II
34 NC_000003.12 3 184734481 + LOC107986163 NNNTCACAGGGATTTGAACA NGG 1 0.5 Tier III
35 NC_000007.14 7 8568917 + LOC105375144 NNNCAACAGGGATTTGATCA NGG 2 0.3235 Tier III
36 NC_000003.12 3 59119657 + CFAP20DC-DT NNNAAACAGGGATTAGAACA NGG 2 0.2757 Tier III
37 NC_000001.11 1 118204056 - LOC105378930 NNNGAACTGGGATTTGAACA NGG 2 0.2679 Tier III
38 NC_000018.10 18 64179796 + LINC01924 NNNTAACAGGGATTTGAACA NAG 1 0.2593 Tier III
39 NC_000005.10 5 89266319 + MEF2C-AS1 NNNTAACAGGGATTTGCACA NGG 1 0.1765 Tier III
40 NC_000008.11 8 71154536 - LOC124901959 NNNGAACAGGGATTTGAACC NGG 2 0.142 Tier III
41 NC_000002.12 2 168406087 + LOC105373734 NNNTAACAGGGAATGGAACA NGG 2 0.0346 Tier III
42 NC_000009.12 9 99310124 + LOC124902231 NNNTAACAGGGATTTGAACA NCA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 18908133 + Pip4k2a NNNTGACAGGGATTTGAACG NGG 2 0.5506 Tier I
2 NC_000086.7 X 151912459 - Huwe1 NNNTAACAGGAACTTGAACA NGG 2 0.7895 Tier II
3 NC_000083.6 17 87022741 + Pigf NNNTAACAGGGAATTAAACA NGG 2 0.6923 Tier II
4 NC_000068.7 2 120148111 - Ehd4 NNNTATCAGTGATTTGAACA NGG 2 0.5804 Tier II
5 NC_000080.6 14 120400296 - Mbnl2 NNNTAAGAGTGATTTGAACA NGG 2 0.3824 Tier II
6 NC_000068.7 2 113002746 - Ryr3 NNNGAACAGGGATTTGAACT NGG 2 0.375 Tier II
7 NC_000067.6 1 70694579 + Spag16 NNNTAACTGTGATTTGAACA NGG 2 0.3482 Tier II
8 NC_000067.6 1 88367834 + Trpm8 NNNTCACAGGGATTAGAACA NGG 2 0.2895 Tier II
9 NC_000067.6 1 64848759 + Plekhm3 NNNGAACAGGCATTTGAACA NGG 2 0.2679 Tier II
10 NC_000077.6 11 54933199 + Tnip1 NNNTAAGAGGGATTTGAAAA NGG 2 0.2017 Tier II
11 NC_000075.6 9 102341608 - Ephb1 NNNTAATAGGGATTTGAACC NGG 2 0.1847 Tier II
12 NC_000077.6 11 70882913 - Rabep1 NNNTAACAGGCATTTGAAAA NGG 2 0.1837 Tier II
13 NC_000077.6 11 80160419 + Adap2 NNNTAGCAGGGATTTGAACC NGG 2 0.1623 Tier II
14 NC_000074.6 8 84430867 - Cacna1a NNNGAACAGGGATTTGAACA NAG 2 0.162 Tier II
15 NC_000082.6 16 65008168 + Htr1f NNNTAACAGTGATTTGGACA NGG 2 0.1434 Tier II
16 NC_000080.6 14 23552234 - Kcnma1 NNNTACCAGGGATCTGAACA NGG 2 0.1299 Tier II
17 NC_000077.6 11 108103863 + Prkca NNNTCACAGGGATTTGAACC NGG 2 0.1136 Tier II
18 NC_000080.6 14 60210611 + Nupl1 NNNTAACTGGGATTTGAACC NGG 2 0.0974 Tier II
19 NC_000075.6 9 32114762 + Arhgap32 NNNTGACAGGGATTTGTACA NGG 2 0.096 Tier II
20 NC_000083.6 17 14286511 + Smoc2 NNNTAAAAGGGATTTGAAGA NGG 2 0.0938 Tier II
21 NC_000070.6 4 87208876 - Slc24a2 NNNTGACAGGGATTTGAACA NTG 2 0.0281 Tier II
22 NC_000080.6 14 60656457 - Spata13 NNNTAACAGGGATTTGAACT NTG 2 0.0234 Tier II
23 NC_000085.6 19 57203602 - Ablim1 NNNTAACAGGCATGTGAACA NGG 2 0.0 Tier II
24 NC_000075.6 9 95859567 + Atr NNNTAACAGGGATTTGAACA NAT 2 0.0 Tier II
25 NC_000067.6 1 90399602 - Gm34992 NNNAAACAGGGGTTTGAACA NGG 2 0.3439 Tier III
26 NC_000079.6 13 57403644 + Gm33294 NNNAAACAGGCATTTGAACA NGG 2 0.2041 Tier III
27 NC_000077.6 11 20920080 + Gm32293 NNNTAACTGGGTTTTGAACA NGG 2 0.1429 Tier III
28 NC_000075.6 9 120504440 - Gm7229 NNNTTACAGGGATTTGAACA NGC 2 0.0081 Tier III
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Other clones with same target sequence:

(none)