Construct: sgRNA BRDN0001148003

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAATCTAATTCAATTAGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRK2 (120892)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77332

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 40293591 + LRRK2 NNNTCTAATTCAATTAGTGT NGG 0 1.0 Tier I
2 NC_000005.10 5 76891339 + S100Z NNNTCCAATTCAATTAGTCT NGG 2 0.4075 Tier II
3 NC_000005.10 5 75079351 + ANKRD31 NNNTCTAATTCAATAAGTTT NGG 2 0.386 Tier II
4 NC_000016.10 16 84224313 + KCNG4 NNNTCTAATTTAATTAATGT NGG 2 0.2872 Tier II
5 NC_000001.11 1 76885638 + ST6GALNAC5 NNNTCTATTTCAATTAGAGT NGG 2 0.2857 Tier II
6 NC_000024.10 Y 12919587 + DDX3Y NNNGCTATTTCAATTAGTGT NGG 2 0.2679 Tier II
7 NC_000010.11 10 95964380 + CC2D2B NNNTCTAATTTAATTAGTAT NGG 2 0.2198 Tier II
8 NC_000013.11 13 29483501 - MTUS2 NNNTCTGATTCAATTAGTGT NAG 2 0.183 Tier II
9 NC_000003.12 3 107682743 - BBX NNNTGTAATTCAATTACTGT NGG 2 0.1412 Tier II
10 NC_000008.11 8 80715696 - ZNF704 NNNTCTAATTTAATTAGTCT NGG 2 0.1379 Tier II
11 NC_000017.11 17 35114849 - RAD51D NNNTCTAATTTAATTAGTCT NGG 2 0.1379 Tier II
12 NC_000007.14 7 26790546 + SKAP2 NNNTATAATTCAATTACTGT NGG 2 0.1345 Tier II
13 NC_000012.12 12 96757122 + CFAP54 NNNTCTATTTTAATTAGTGT NGG 2 0.1319 Tier II
14 NC_000013.11 13 110419832 - COL4A2 NNNTCTAATTCAATCAGTCT NGG 2 0.1223 Tier II
15 NC_000004.12 4 21435754 - KCNIP4 NNNTTTAATTCAATTAGTGG NGG 2 0.1123 Tier II
16 NC_000002.12 2 179738720 + ZNF385B NNNTCTAATTTATTTAGTGT NGG 2 0.0923 Tier II
17 NC_000003.12 3 19413274 + KCNH8 NNNTCTAATTAAATTAGTGC NGG 2 0.0682 Tier II
18 NC_000001.11 1 237787133 + RYR2 NNNTCTAATTCCATTATTGT NGG 2 0.0658 Tier II
19 NC_000003.12 3 7465334 + GRM7 NNNTTTAATTCAATTAGTGT NGC 2 0.0141 Tier II
20 NC_000010.11 10 101442248 - BTRC NNNTCTAATTCAATTAGTGC NGT 2 0.0015 Tier II
21 NC_000006.12 6 126038579 + TRMT11 NNNTCTAATTCAAGTAGTGA NGG 2 0.0 Tier II
22 NC_000007.14 7 89948606 + STEAP2-AS1 NNNTCAAATTCAATTAGTGT NGG 1 0.8667 Tier III
23 NC_000007.14 7 137077736 - LOC349160 NNNTCTAATTCAATTAATGA NGG 2 0.525 Tier III
24 NC_000010.11 10 121058303 - LOC105378521 NNNTTTAATCCAATTAGTGT NGG 2 0.3394 Tier III
25 NC_000004.12 4 78736048 - LOC101928893 NNNTCTGATTCAATTAGTCT NGG 2 0.3164 Tier III
26 NC_000016.10 16 60025634 + LINC02141 NNNTCTAATTTGATTAGTGT NGG 2 0.2222 Tier III
27 NC_000010.11 10 95964380 + ENTPD1-AS1 NNNTCTAATTTAATTAGTAT NGG 2 0.2198 Tier III
28 NC_000002.12 2 156176418 + LINC01876 NNNTCTGATTTAATTAGTGT NGG 2 0.2172 Tier III
29 NC_000002.12 2 170694193 + LOC100130256 NNNTCTGATTTAATTAGTGT NGG 2 0.2172 Tier III
30 NC_000020.11 20 57309782 + LOC105372687 NNNTCTAATTCTATTAGTGA NGG 2 0.1875 Tier III
31 NC_000013.11 13 29483501 - MTUS2-AS1 NNNTCTGATTCAATTAGTGT NAG 2 0.183 Tier III
32 NC_000017.11 17 35114849 - RAD51L3-RFFL NNNTCTAATTTAATTAGTCT NGG 2 0.1379 Tier III
33 NC_000010.11 10 53016451 + LINC02672 NNNTCTAATTCACTTAGTGA NGG 2 0.1184 Tier III
34 NC_000002.12 2 220030224 - LOC105373891 NNNTCTACTTCACTTAGTGT NGG 2 0.0902 Tier III
35 NC_000008.11 8 122479898 - SMILR NNNTCTAATTCACTTAGGGT NGG 2 0.0702 Tier III
36 NC_000015.10 15 87516302 - LOC102724465 NNNTCTAATTCACTTAGGGT NGG 2 0.0702 Tier III
37 NC_000003.12 3 19413274 + LOC105376982 NNNTCTAATTAAATTAGTGC NGG 2 0.0682 Tier III
38 NC_000008.11 8 127090779 + PRNCR1 NNNTCTAATTCAATTAGTAT NGT 2 0.0115 Tier III
39 NC_000005.10 5 81897868 + LOC124901018 NNNTATAATTCAATTAGTGT NGT 2 0.0092 Tier III
40 NC_000005.10 5 106859112 - LINC01950 NNNTCTAATTCAATGAGTGT NGT 2 0.0008 Tier III
41 NC_000004.12 4 129762604 + LINC02466 NNNTCTAATTCATTTTGTGT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 91734035 + Lrrk2 NNNTCCAACTCAATTAGTGT NGG 2 0.8392 Tier I
2 NC_000074.6 8 46117030 - Snx25 NNNTCTTATTCAATTAGTAT NGG 2 0.3125 Tier II
3 NC_000068.7 2 71226341 - Dync1i2 NNNTGTAATTCAATTAGTCT NGG 2 0.269 Tier II
4 NC_000073.6 7 30135780 - Zfp382 NNNTCTAACTCAATTACTGT NGG 2 0.2172 Tier II
5 NC_000082.6 16 50300970 + Bbx NNNTGTAATTCAATTATTGT NGG 2 0.15 Tier II
6 NC_000079.6 13 88885623 + Edil3 NNNTCTAAGTCAATTAGTGC NGG 2 0.0563 Tier II
7 NC_000084.6 18 19972310 + Dsc3 NNNTCTAATTCAATGAGTAT NGG 2 0.0357 Tier II
8 NC_000079.6 13 109241568 + Pde4d NNNTCTATTTCAATTAGTGT NGC 2 0.0095 Tier II
9 NC_000067.6 1 41508643 - Gm39631 NNNTCTACTTAAATTAGTGT NGG 2 0.3214 Tier III
10 NC_000079.6 13 88885623 + Gm46433 NNNTCTAAGTCAATTAGTGC NGG 2 0.0563 Tier III
11 NC_000075.6 9 100290389 - Gm28167 NNNTCTAATTCAATGAATGT NGG 2 0.0467 Tier III
12 NC_000082.6 16 26306917 + Gm41436 NNNTCTAATTCAATTTGTGA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)