Construct: sgRNA BRDN0001148005
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGGCCCAGCTAGAGCGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PASK (23178)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76205
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
241127191 |
- |
PASK |
NNNGCCCAGCTAGAGCGGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
66999164 |
+ |
TYW1 |
NNNGCCCAGTTAGAGCTGAT |
NGG |
2 |
0.2353 |
Tier II |
| 3 |
NC_000019.10 |
19 |
5605592 |
- |
SAFB2 |
NNNGCCCAGCTGGAGTGGAT |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000001.11 |
1 |
51945700 |
+ |
RAB3B |
NNNGCCCTGCTAGAGTGGAT |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000019.10 |
19 |
14413328 |
- |
DDX39A |
NNNGCCCAGGTAGAGCTGAT |
NGG |
2 |
0.0972 |
Tier II |
| 6 |
NC_000003.12 |
3 |
46687416 |
+ |
ALS2CL |
NNNGCCCAGCTAGAGCGGGT |
NTG |
2 |
0.0146 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
10984370 |
- |
Ms4a15 |
NNNGCCCAGCAAGAGCGGAT |
NGG |
1 |
0.75 |
Tier II |
| 2 |
NC_000085.6 |
19 |
4922727 |
- |
Dpp3 |
NNNGGCCAGCTAGAGAGGAT |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000073.6 |
7 |
24134337 |
- |
Zfp235 |
NNNGCCCAGCTAGAGAGGAC |
NGG |
2 |
0.0909 |
Tier II |
| 4 |
NC_000073.6 |
7 |
35312897 |
- |
Gpatch1 |
NNNGCCCAGCTACAGGGGAT |
NGG |
2 |
0.0648 |
Tier II |
| 5 |
NC_000085.6 |
19 |
24682858 |
+ |
Pgm5 |
NNNGCCCAGCTAGAGCTGAT |
NAG |
2 |
0.0648 |
Tier II |
| 6 |
NC_000085.6 |
19 |
6014945 |
+ |
Capn1 |
NNNGCCCAGCTACAGCGGAC |
NGG |
2 |
0.0383 |
Tier II |
| 7 |
NC_000085.6 |
19 |
6014945 |
+ |
Gm10814 |
NNNGCCCAGCTACAGCGGAC |
NGG |
2 |
0.0383 |
Tier III |
Other clones with same target sequence:
(none)
