Construct: sgRNA BRDN0001148007
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGTGGGTCATCATCGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTBK1 (84630)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75803
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
43263004 |
+ |
TTBK1 |
NNNGTGGGTCATCATCGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
76008080 |
- |
EVPL |
NNNGAGGGGCATCATCGACA |
NGG |
2 |
0.3095 |
Tier I |
| 3 |
NC_000005.10 |
5 |
156208786 |
+ |
SGCD |
NNNGTTGGTCATCATCAACA |
NGG |
2 |
0.6222 |
Tier II |
| 4 |
NC_000006.12 |
6 |
62099555 |
- |
KHDRBS2 |
NNNGTGGGTTATCATGGACA |
NGG |
2 |
0.1448 |
Tier II |
| 5 |
NC_000006.12 |
6 |
37331959 |
- |
TBC1D22B |
NNNGTGGGTCATCATGGAAA |
NGG |
2 |
0.0659 |
Tier II |
| 6 |
NC_000006.12 |
6 |
151999389 |
+ |
ESR1 |
NNNGTGAGTCATCATCGACA |
NTG |
2 |
0.039 |
Tier II |
| 7 |
NC_000017.11 |
17 |
3682366 |
- |
P2RX5 |
NNNGTGGGTCGTCATCGACA |
NGC |
2 |
0.0144 |
Tier II |
| 8 |
NC_000022.11 |
22 |
31866038 |
- |
DEPDC5 |
NNNGTGGGTCATCAGCTACA |
NGG |
2 |
0.0125 |
Tier II |
| 9 |
NC_000017.11 |
17 |
3682366 |
- |
P2RX5-TAX1BP3 |
NNNGTGGGTCGTCATCGACA |
NGC |
2 |
0.0144 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
46467473 |
- |
Ttbk1 |
NNNGTGGGTCATTATTGACA |
NGG |
2 |
0.2154 |
Tier I |
| 2 |
NC_000078.6 |
12 |
89316409 |
+ |
Nrxn3 |
NNNGTGGGTCATCTTCAACA |
NGG |
2 |
0.4978 |
Tier II |
| 3 |
NC_000071.6 |
5 |
71719221 |
+ |
Gabrb1 |
NNNGTGGGTCAGCATCAACA |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000074.6 |
8 |
93126884 |
- |
Ces1c |
NNNGTGGGTCCTCATAGACA |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000069.6 |
3 |
41650759 |
+ |
Sclt1 |
NNNGTGGGTCATGATCCACA |
NGG |
2 |
0.0321 |
Tier II |
| 6 |
NC_000083.6 |
17 |
12789899 |
+ |
Airn |
NNNCTGGGTCATCATAGACA |
NGG |
2 |
0.5294 |
Tier III |
Other clones with same target sequence:
(none)
