Construct: sgRNA BRDN0001148014

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGCAAACATAACAACCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKM (1158)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78028

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 45319629 - CKM NNNCAAACATAACAACCACA NGG 0 1.0 Tier I
2 NC_000022.11 22 50128123 - MOV10L1 NNNCAAACAAAACAACCACA NGG 1 0.8571 Tier II
3 NC_000010.11 10 32454329 - CCDC7 NNNAAAACATAATAACCACA NGG 2 0.5895 Tier II
4 NC_000014.9 14 93112924 + ITPK1 NNNCAAACAAAACAACCTCA NGG 2 0.4286 Tier II
5 NC_000003.12 3 19928449 - EFHB NNNCAAACAAAACAACAACA NGG 2 0.4 Tier II
6 NC_000007.14 7 140053895 + PARP12 NNNCAAATATCACAACCACA NGG 2 0.35 Tier II
7 NC_000002.12 2 199668635 - FTCDNL1 NNNCAAACATAACAACCAAG NGG 2 0.3277 Tier II
8 NC_000008.11 8 100920550 + YWHAZ NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier II
9 NC_000011.10 11 3971904 + STIM1 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier II
10 NC_000015.10 15 45056607 - SORD NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier II
11 NC_000009.12 9 122746503 - OR1L6 NNNCAAACAAAACAACCACA NAG 2 0.2222 Tier II
12 NC_000005.10 5 178483623 + COL23A1 NNNCAAACCTAAAAACCACA NGG 2 0.2198 Tier II
13 NC_000008.11 8 123814180 - FAM91A1 NNNCAAACATAACATACACA NGG 2 0.2 Tier II
14 NC_000020.11 20 53359685 - TSHZ2 NNNCGAACATACCAACCACA NGG 2 0.1895 Tier II
15 NC_000004.12 4 38880263 - FAM114A1 NNNCAAACATTACAACCACT NGG 2 0.1846 Tier II
16 NC_000002.12 2 1172132 - SNTG2 NNNCAATCATCACAACCACA NGG 2 0.175 Tier II
17 NC_000007.14 7 36272568 - EEPD1 NNNCAAAGATACCAACCACA NGG 2 0.1692 Tier II
18 NC_000002.12 2 229075665 + PID1 NNNCAAACACAACAATCACA NGG 2 0.1641 Tier II
19 NC_000001.11 1 81339575 - ADGRL2 NNNCAAACAAAACAACCCCA NGG 2 0.1633 Tier II
20 NC_000006.12 6 20128388 + MBOAT1 NNNTAAACATAACAACCCCA NGG 2 0.1524 Tier II
21 NC_000004.12 4 93316489 + GRID2 NNNCACACATATCAACCACA NGG 2 0.1515 Tier II
22 NC_000013.11 13 25500031 + ATP8A2 NNNCAAACATGACAACCCCA NGG 2 0.1238 Tier II
23 NC_000010.11 10 24412116 - KIAA1217 NNNCAAACATAAAAATCACA NGG 2 0.1183 Tier II
24 NC_000017.11 17 81036725 - BAIAP2 NNNCAAACCTAACAACCCCA NGG 2 0.1088 Tier II
25 NC_000002.12 2 211433079 + ERBB4 NNNCAAACAGAACAAGCACA NGG 2 0.0769 Tier II
26 NC_000001.11 1 177206984 + BRINP2 NNNAAAACATAACAACCACA NGA 2 0.0585 Tier II
27 NC_000001.11 1 74151391 + LRRIQ3 NNNCAAACATAACAACTAGA NGG 2 0.0583 Tier II
28 NC_000015.10 15 62778188 + TLN2 NNNCAAACATAACAAGCCCA NGG 2 0.0293 Tier II
29 NC_000010.11 10 101113698 + TLX1NB NNNCAAAAATAACAAACACA NGG 2 0.65 Tier III
30 NC_000013.11 13 94888811 - LOC101927284 NNNTAAACATAACAACAACA NGG 2 0.3733 Tier III
31 NC_000011.10 11 30277788 - ARL14EP-DT NNNTAAACATAACAACTACA NGG 2 0.3733 Tier III
32 NC_000002.12 2 51771306 - NRXN1-DT NNNCAAAGACAACAACCACA NGG 2 0.3429 Tier III
33 NC_000009.12 9 127883669 + ST6GALNAC4-ST6GALNAC6-AK1 NNNCAAACAAAAAAACCACA NGG 2 0.3297 Tier III
34 NC_000011.10 11 41832639 - LINC01499 NNNCAAACAAAAAAACCACA NGG 2 0.3297 Tier III
35 NC_000002.12 2 126557287 + YWHAZP2 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier III
36 NC_000002.12 2 126557287 + LOC105373602 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier III
37 NC_000006.12 6 127356933 - YWHAZP4 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier III
38 NC_000010.11 10 105687202 - YWHAZP5 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier III
39 NC_000010.11 10 105687202 - LINC02627 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier III
40 NC_000011.10 11 60078597 - YWHAZP9 NNNTAAACATAAAAACCACA NGG 2 0.3077 Tier III
41 NC_000006.12 6 7682169 - LOC105374906 NNNCAAAGATATCAACCACA NGG 2 0.2143 Tier III
42 NC_000001.11 1 120987437 - FAM91A4P NNNCAAACATAACATACACA NGG 2 0.2 Tier III
43 NC_000001.11 1 120987437 - PDE4DIPP4 NNNCAAACATAACATACACA NGG 2 0.2 Tier III
44 NC_000001.11 1 143770562 - FAM91A3P NNNCAAACATAACATACACA NGG 2 0.2 Tier III
45 NC_000001.11 1 149679221 - LINC00869 NNNCAAACATAACATACACA NGG 2 0.2 Tier III
46 NC_000001.11 1 149679221 - FAM91A2P NNNCAAACATAACATACACA NGG 2 0.2 Tier III
47 NC_000008.11 8 64332567 - LINC01414 NNNCAAACAAAACATCCACA NGG 2 0.1714 Tier III
48 NC_000002.12 2 104152988 - LOC105373523 NNNCAAACATAGCAACCAGA NGG 2 0.0903 Tier III
49 NC_000006.12 6 84778905 - LOC124901494 NNNCAAAAATAACAACCACA NGC 2 0.0144 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 87810475 - Olfr1145 NNNCAACCATAACAACCACA NAG 2 0.1134 Tier I
2 NC_000071.6 5 44020884 + Prom1 NNNCAAACGTAAGAACCACA NGG 2 0.0909 Tier I
3 NC_000081.6 15 43157374 - Rspo2 NNNCAAAGATAACAAACACA NGG 2 0.6429 Tier II
4 NC_000068.7 2 29976504 - Sptan1 NNNCAAACATAACAAACACT NGG 2 0.6 Tier II
5 NC_000068.7 2 68893818 - Cers6 NNNCAAATCTAACAACCACA NGG 2 0.5 Tier II
6 NC_000073.6 7 58767197 + Atp10a NNNCAAACAAAACAACCTCA NGG 2 0.4286 Tier II
7 NC_000080.6 14 47269045 + Wdhd1 NNNCAAACAAAACAACTACA NGG 2 0.4 Tier II
8 NC_000071.6 5 34467243 - Fam193a NNNCAAACAAAACAACCAAA NGG 2 0.3673 Tier II
9 NC_000077.6 11 75154495 - Smg6 NNNGAAACATAGCAACCACA NGG 2 0.3611 Tier II
10 NC_000081.6 15 76278214 - Spatc1 NNNCAAACATAGCAACCATA NGG 2 0.3333 Tier II
11 NC_000072.6 6 114703519 - Atg7 NNNCAAACAAAAAAACCACA NGG 2 0.3297 Tier II
12 NC_000076.6 10 74598516 - Pcdh15 NNNCAAACAAAAAAACCACA NGG 2 0.3297 Tier II
13 NC_000081.6 15 36772574 + Ywhaz NNNAAAACATAAAAACCACA NGG 2 0.3239 Tier II
14 NC_000086.7 X 143788072 - Pak3 NNNCAAACATGACAACCAAA NGG 2 0.2786 Tier II
15 NC_000075.6 9 40361873 + Gramd1b NNNCAAACATAGAAACCACA NGG 2 0.2778 Tier II
16 NC_000080.6 14 52464806 - Olfr1508 NNNCAAACATCACAGCCACA NGG 2 0.26 Tier II
17 NC_000070.6 4 32929046 + Ankrd6 NNNCAAACATAAAAGCCACA NGG 2 0.25 Tier II
18 NC_000068.7 2 132256638 - Cds2 NNNCAATCCTAACAACCACA NGG 2 0.25 Tier II
19 NC_000079.6 13 75898756 + Rhobtb3 NNNCAAAGATAAAAACCACA NGG 2 0.2473 Tier II
20 NC_000082.6 16 48552364 - Morc1 NNNCAAACAAAACAACCACA NAG 2 0.2222 Tier II
21 NC_000073.6 7 141081416 - Pkp3 NNNCAAACAAAACAACCACC NGG 2 0.1948 Tier II
22 NC_000071.6 5 115239845 - Pop5 NNNCAGACATAACAACCACC NGG 2 0.1623 Tier II
23 NC_000082.6 16 40373558 + Lsamp NNNCAAACATAACAAGCACA NGG 1 0.1538 Tier II
24 NC_000079.6 13 106901149 - Ipo11 NNNCAACCATTACAACCACA NGG 2 0.1346 Tier II
25 NC_000072.6 6 143068833 + C2cd5 NNNCAAACATAACAATCAAA NGG 2 0.1319 Tier II
26 NC_000077.6 11 44798346 - Ebf1 NNNAAAACATAACAAGCACA NGG 2 0.1296 Tier II
27 NC_000069.6 3 145995803 - Syde2 NNNCAAAGATAACAAGCACA NGG 2 0.0989 Tier II
28 NC_000082.6 16 35240337 + Adcy5 NNNCAATCATAACCACCACA NGG 2 0.0938 Tier II
29 NC_000071.6 5 31457629 - Zfp512 NNNCAAACATAACACCCACA NAG 2 0.0707 Tier II
30 NC_000072.6 6 118807828 + Cacna1c NNNCAAACATAAGAACCACA NAG 2 0.0354 Tier II
31 NC_000076.6 10 128286107 - Stat2 NNNCAAACAGAACAACCACA NGA 2 0.0347 Tier II
32 NC_000076.6 10 90514757 + Anks1b NNNCACACATAACAACCACA NGA 2 0.0316 Tier II
33 NC_000086.7 X 83703569 - Dmd NNNCAAACATAAAAACCACA NGA 2 0.0267 Tier II
34 NC_000075.6 9 89604909 + Minar1 NNNCAAACACAACAACCACA NTG 2 0.0208 Tier II
35 NC_000070.6 4 58170816 + Svep1 NNNCAAACATACCAACCACA NTG 2 0.0103 Tier II
36 NC_000074.6 8 16649445 + Csmd1 NNNCAAACATAACAACCACA NAC 2 0.0 Tier II
37 NC_000073.6 7 94692269 + Gm32647 NNNCAAACAAAACAACCACT NGG 2 0.5143 Tier III
38 NC_000084.6 18 32512583 + Gm44577 NNNCAAACAAAACAACAACA NGG 2 0.4 Tier III
39 NC_000076.6 10 54193957 - Gm40651 NNNAAAACATAACAACCAAA NGG 2 0.3609 Tier III
40 NC_000080.6 14 52464806 - Tcra NNNCAAACATCACAGCCACA NGG 2 0.26 Tier III
41 NC_000076.6 10 118783375 - Gm33439 NNNCAAACAAAACAACCACA NAG 2 0.2222 Tier III
42 NC_000079.6 13 40996847 + A730081D07Rik NNNCAAACAAAACAACCACA NAG 2 0.2222 Tier III
43 NC_000079.6 13 84877732 - Gm34196 NNNCCAACATAACAAGCACA NGG 2 0.0769 Tier III
44 NC_000076.6 10 99268003 - Gm34921 NNNCAAACATAAAAACCACA NGC 2 0.0085 Tier III
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Other clones with same target sequence:

(none)