Construct: sgRNA BRDN0001148018
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCCTACCCACCATACCAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DLG1 (1739)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76618
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
197161645 |
- |
DLG1 |
NNNCCTACCCACCATACCAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
143185664 |
+ |
USP38 |
NNNCCTACGCACGATACCAC |
NGG |
2 |
0.0844 |
Tier I |
| 3 |
NC_000019.10 |
19 |
18511630 |
+ |
ELL |
NNNACCACCCACCATACCAC |
NGG |
2 |
0.7656 |
Tier II |
| 4 |
NC_000009.12 |
9 |
88067560 |
+ |
LOC124902201 |
NNNCCTAACCACCAAACCAC |
NGG |
2 |
0.3763 |
Tier II |
| 5 |
NC_000022.11 |
22 |
41978305 |
- |
SEPTIN3 |
NNNTCTACCCAGCATACCAC |
NGG |
2 |
0.3556 |
Tier II |
| 6 |
NC_000003.12 |
3 |
54960354 |
+ |
CACNA2D3 |
NNNCCAACCCCCCATACCAC |
NGG |
2 |
0.3467 |
Tier II |
| 7 |
NC_000003.12 |
3 |
54960354 |
+ |
LRTM1 |
NNNCCAACCCCCCATACCAC |
NGG |
2 |
0.3467 |
Tier II |
| 8 |
NC_000011.10 |
11 |
78701222 |
- |
TENM4 |
NNNCCTGCCCAGCATACCAC |
NGG |
2 |
0.3137 |
Tier II |
| 9 |
NC_000001.11 |
1 |
95167253 |
+ |
TLCD4 |
NNNCCTGCCCTCCATACCAC |
NGG |
2 |
0.2172 |
Tier II |
| 10 |
NC_000001.11 |
1 |
95167253 |
+ |
TLCD4-RWDD3 |
NNNCCTGCCCTCCATACCAC |
NGG |
2 |
0.2172 |
Tier II |
| 11 |
NC_000010.11 |
10 |
71283512 |
+ |
UNC5B |
NNNCTTACCCACCATGCCAC |
NGG |
2 |
0.1224 |
Tier II |
| 12 |
NC_000001.11 |
1 |
11115153 |
- |
MTOR |
NNNCCTACCCTCCATACCAC |
NTG |
2 |
0.012 |
Tier II |
| 13 |
NC_000007.14 |
7 |
101543104 |
+ |
COL26A1 |
NNNCCTCCCCACCATACCAC |
NGT |
2 |
0.0071 |
Tier II |
| 14 |
NC_000007.14 |
7 |
149213172 |
+ |
ZNF282 |
NNNCCTACCCACCATACCAG |
NGA |
2 |
0.0041 |
Tier II |
| 15 |
NC_000002.12 |
2 |
226794278 |
- |
IRS1 |
NNNCCGACCCACCATCCCAC |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000016.10 |
16 |
78244124 |
- |
WWOX |
NNNCCTACCCACCATTGCAC |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000008.11 |
8 |
33758471 |
+ |
LOC105379364 |
NNNACTACCCACCATACCTC |
NGG |
2 |
0.4534 |
Tier III |
| 18 |
NC_000009.12 |
9 |
88067560 |
+ |
LINC03026 |
NNNCCTAACCACCAAACCAC |
NGG |
2 |
0.3763 |
Tier III |
| 19 |
NC_000001.11 |
1 |
95167253 |
+ |
RWDD3-DT |
NNNCCTGCCCTCCATACCAC |
NGG |
2 |
0.2172 |
Tier III |
| 20 |
NC_000004.12 |
4 |
55385607 |
- |
LINC02928 |
NNNCATACCCACCATACGAC |
NGG |
2 |
0.0762 |
Tier III |
| 21 |
NC_000003.12 |
3 |
28684459 |
- |
LINC00693 |
NNNCCTAGCCACCATCCCAC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
74836717 |
+ |
Mnt |
NNNCCTGCCCACCAGACCAC |
NGG |
2 |
0.0353 |
Tier I |
| 2 |
NC_000074.6 |
8 |
69619938 |
+ |
Zfp868 |
NNNCCTCCCTACCATACCAC |
NGG |
2 |
0.4118 |
Tier II |
| 3 |
NC_000079.6 |
13 |
24198732 |
+ |
Carmil1 |
NNNCCCTCCCACCATACCAC |
NGG |
2 |
0.3977 |
Tier II |
| 4 |
NC_000079.6 |
13 |
24198564 |
+ |
Carmil1 |
NNNACTTCCCACCATACCAC |
NGG |
2 |
0.3684 |
Tier II |
| 5 |
NC_000084.6 |
18 |
77232688 |
- |
St8sia5 |
NNNCCAACCCACCCTACCAC |
NGG |
2 |
0.1857 |
Tier II |
| 6 |
NC_000070.6 |
4 |
134865185 |
- |
Rhd |
NNNCCTCCCCACCACACCAC |
NGG |
2 |
0.1193 |
Tier II |
| 7 |
NC_000086.7 |
X |
142811062 |
+ |
Tmem164 |
NNNCCTACCCTCCACACCAC |
NGG |
2 |
0.0839 |
Tier II |
| 8 |
NC_000072.6 |
6 |
145419034 |
+ |
Lmntd1 |
NNNCCAACCCACCATAGCAC |
NGG |
2 |
0.051 |
Tier II |
| 9 |
NC_000067.6 |
1 |
132063638 |
+ |
Mfsd4a |
NNNCCTACCCACCATACCAA |
NTG |
2 |
0.0195 |
Tier II |
| 10 |
NC_000084.6 |
18 |
5690045 |
- |
Zeb1 |
NNNCCCACCCACCATACCAC |
NGT |
2 |
0.0147 |
Tier II |
| 11 |
NC_000078.6 |
12 |
71625994 |
+ |
9630002D21Rik |
NNNCCCACCCACCAAACCAC |
NGG |
2 |
0.5263 |
Tier III |
| 12 |
NC_000076.6 |
10 |
92441104 |
+ |
Gm40738 |
NNNCCTCCCCACCATACCAC |
NGT |
2 |
0.0071 |
Tier III |
Other clones with same target sequence:
(none)
