Construct: sgRNA BRDN0001148019
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGATGGCAACCTTCACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TJP2 (9414)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77828
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
69225386 |
+ |
TJP2 |
NNNGATGGCAACCTTCACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
142246907 |
- |
TSNARE1 |
NNNGATGGCAGCCTTCATGA |
NGG |
2 |
0.4179 |
Tier II |
| 3 |
NC_000022.11 |
22 |
31865739 |
+ |
DEPDC5 |
NNNGATGGCAAGCTTCATGA |
NGG |
2 |
0.2857 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
24125727 |
- |
Tjp2 |
NNNGATGGCAACCTTCATGA |
NGG |
1 |
0.6429 |
Tier I |
| 2 |
NC_000086.7 |
X |
147169738 |
- |
Htr2c |
NNNGATGGCAATCTTCATGA |
NGG |
2 |
0.3462 |
Tier I |
| 3 |
NC_000084.6 |
18 |
35235603 |
- |
Ctnna1 |
NNNGTTGGCAACCTGCACGA |
NGG |
2 |
0.0182 |
Tier I |
| 4 |
NC_000073.6 |
7 |
25683244 |
- |
B9d2 |
NNNGATAGCAACCTTCAAGA |
NGG |
2 |
0.5385 |
Tier II |
| 5 |
NC_000067.6 |
1 |
154853610 |
+ |
Cacna1e |
NNNCATGGCAACCTTCACAA |
NGG |
2 |
0.3782 |
Tier II |
| 6 |
NC_000075.6 |
9 |
118878387 |
+ |
Itga9 |
NNNGATGGCAGCATTCACGA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000080.6 |
14 |
10288159 |
+ |
Fhit |
NNNGATGGCAACCTTCAGAA |
NGG |
2 |
0.0952 |
Tier II |
| 8 |
NC_000073.6 |
7 |
105700647 |
+ |
Dnhd1 |
NNNGATTGCAACCTTCAGGA |
NGG |
2 |
0.0762 |
Tier II |
| 9 |
NC_000080.6 |
14 |
28269206 |
+ |
Erc2 |
NNNGATGACAACCTTCACGA |
NGA |
2 |
0.0694 |
Tier II |
| 10 |
NC_000076.6 |
10 |
49384568 |
- |
Grik2 |
NNNGATGGCAACCTTCAAGA |
NTG |
2 |
0.021 |
Tier II |
| 11 |
NC_000081.6 |
15 |
87633951 |
+ |
Tafa5 |
NNNGCTGGCAACCTTCACGA |
NGC |
2 |
0.0111 |
Tier II |
| 12 |
NC_000077.6 |
11 |
67136063 |
+ |
Gm31285 |
NNNGATGGCAGCCTTCACAA |
NGG |
2 |
0.4643 |
Tier III |
Other clones with same target sequence:
(none)
