Construct: sgRNA BRDN0001148020
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCCAAAGCGCTAATTCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K7 (6885)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75693
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
90560098 |
- |
MAP3K7 |
NNNCAAAGCGCTAATTCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
208151170 |
+ |
PLXNA2 |
NNNCAAAGCAATAATTCACA |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000006.12 |
6 |
64147275 |
+ |
EYS |
NNNCAAATCACTAATTCACA |
NGG |
2 |
0.5833 |
Tier II |
| 4 |
NC_000011.10 |
11 |
79367188 |
+ |
TENM4 |
NNNCTAAGGGCTAATTCACA |
NGG |
2 |
0.2251 |
Tier II |
| 5 |
NC_000002.12 |
2 |
137215745 |
- |
THSD7B |
NNNCAAAGAGCTAATTCACA |
NAG |
2 |
0.2222 |
Tier II |
| 6 |
NC_000003.12 |
3 |
155845582 |
+ |
SLC33A1 |
NNNCAAAGCCCTAATTCAAA |
NGG |
2 |
0.1714 |
Tier II |
| 7 |
NC_000013.11 |
13 |
65913051 |
- |
LOC105370243 |
NNNCAAAGCACTACTTCACA |
NGG |
2 |
0.2 |
Tier III |
| 8 |
NC_000021.9 |
21 |
15114564 |
+ |
LOC107985483 |
NNNGAAAGCCCTAATTCACA |
NGG |
2 |
0.2 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
31979853 |
+ |
Map3k7 |
NNNCAAAGCGCTGATTCACA |
NGG |
1 |
0.6522 |
Tier I |
| 2 |
NC_000079.6 |
13 |
63230604 |
- |
Aopep |
NNNCAAAGCACTAATTAACA |
NGG |
2 |
0.4356 |
Tier II |
| 3 |
NC_000067.6 |
1 |
182807501 |
+ |
Susd4 |
NNNCAAAGTGCTAATGCACA |
NGG |
2 |
0.3029 |
Tier II |
| 4 |
NC_000071.6 |
5 |
136374398 |
- |
Cux1 |
NNNCAAAGCGCTGATGCACA |
NGG |
2 |
0.2258 |
Tier II |
| 5 |
NC_000070.6 |
4 |
19019202 |
- |
Cnbd1 |
NNNCAAAGAGCTAATTCACA |
NGA |
2 |
0.0595 |
Tier II |
| 6 |
NC_000067.6 |
1 |
10397973 |
+ |
Cpa6 |
NNNAAAAGCGCTAATTGACA |
NGG |
2 |
0.0495 |
Tier II |
| 7 |
NC_000079.6 |
13 |
34332963 |
- |
Slc22a23 |
NNNCAAAGCGCTGAGTCACA |
NGG |
2 |
0.0326 |
Tier II |
| 8 |
NC_000067.6 |
1 |
44131412 |
- |
Bivm |
NNNCAAAGCGCTACTTCAGA |
NGG |
2 |
0.0268 |
Tier II |
| 9 |
NC_000076.6 |
10 |
82431122 |
+ |
Gm8188 |
NNNCAAAGCTCTAATTCACA |
NGG |
1 |
0.8125 |
Tier III |
Other clones with same target sequence:
(none)
