Construct: sgRNA BRDN0001148022
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGCTTCACAATGTAGGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK10 (6793)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75487
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
172156673 |
+ |
STK10 |
NNNGCTTCACAATGTAGGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
166510300 |
+ |
RPS6KA2 |
NNNGCTTCACAATGAAGGGG |
NGA |
2 |
0.0402 |
Tier I |
| 3 |
NC_000001.11 |
1 |
161013951 |
- |
F11R |
NNNGTTTCACAATGTAGGGG |
NGG |
1 |
0.6364 |
Tier II |
| 4 |
NC_000004.12 |
4 |
174739300 |
- |
GLRA3 |
NNNGCCCCACAATGTAGGGG |
NGG |
2 |
0.625 |
Tier II |
| 5 |
NC_000009.12 |
9 |
136055545 |
- |
NACC2 |
NNNGCTTTACAATCTAGGGG |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000018.10 |
18 |
65800898 |
- |
CDH7 |
NNNGTTTCACAATGAAGGGG |
NGG |
2 |
0.3684 |
Tier II |
| 7 |
NC_000007.14 |
7 |
111775744 |
+ |
DOCK4 |
NNNGCTGCACACTGTAGGGG |
NGG |
2 |
0.1548 |
Tier II |
| 8 |
NC_000003.12 |
3 |
97556076 |
- |
EPHA6 |
NNNGCTTCAGAATGTAGGGG |
NTG |
2 |
0.0152 |
Tier II |
| 9 |
NC_000014.9 |
14 |
62484700 |
- |
LOC105370528 |
NNNGCTTCACAATGCAGAGG |
NGG |
2 |
0.1888 |
Tier III |
| 10 |
NC_000014.9 |
14 |
62484700 |
- |
LOC105370529 |
NNNGCTTCACAATGCAGAGG |
NGG |
2 |
0.1888 |
Tier III |
| 11 |
NC_000014.9 |
14 |
54094110 |
+ |
LOC105370507 |
NNNGCTTCACACTGTAGGGG |
NGA |
2 |
0.0183 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
71554815 |
- |
Fam135b |
NNNGCTTCACTGTGTAGGGG |
NGG |
2 |
0.2222 |
Tier II |
| 2 |
NC_000085.6 |
19 |
7614964 |
+ |
Plaat5 |
NNNGCTTCACAATGTGGGGG |
NGG |
1 |
0.1923 |
Tier II |
| 3 |
NC_000078.6 |
12 |
112916306 |
- |
Jag2 |
NNNGCTTCACAAGGTAGGAG |
NGG |
2 |
0.1863 |
Tier II |
| 4 |
NC_000083.6 |
17 |
3656236 |
+ |
Nox3 |
NNNGCTTCACCATGTAGGCG |
NGG |
2 |
0.1793 |
Tier II |
| 5 |
NC_000067.6 |
1 |
80607611 |
- |
Dock10 |
NNNGCTTCAGAATGCAGGGG |
NGG |
2 |
0.1061 |
Tier II |
| 6 |
NC_000075.6 |
9 |
49446075 |
+ |
Ttc12 |
NNNGCTTCACAATGTTGGGG |
NGG |
1 |
0.0 |
Tier II |
| 7 |
NC_000073.6 |
7 |
61052587 |
+ |
A230006K03Rik |
NNNCCTTCAAAATGTAGGGG |
NGG |
2 |
0.4588 |
Tier III |
| 8 |
NC_000070.6 |
4 |
114727559 |
- |
Gm33655 |
NNNGCTTCACAGTCTAGGGG |
NGG |
2 |
0.3095 |
Tier III |
| 9 |
NC_000084.6 |
18 |
26038069 |
- |
Gm33228 |
NNNGCTTCACAATTTAGGGG |
NGC |
2 |
0.0059 |
Tier III |
Other clones with same target sequence:
(none)
