Construct: sgRNA BRDN0001148023
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGCCCAATGAAATCATCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HKDC1 (80201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76830
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
69232894 |
+ |
HKDC1 |
NNNCCCAATGAAATCATCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
62242730 |
+ |
PTPRG |
NNNCCCAATGGAATCATCCC |
NGG |
2 |
0.2786 |
Tier II |
| 3 |
NC_000023.11 |
X |
132785430 |
+ |
HS6ST2 |
NNNCCCAATCAAATCATCCC |
NGG |
2 |
0.1714 |
Tier II |
| 4 |
NC_000005.10 |
5 |
15840441 |
- |
FBXL7 |
NNNCCCAATGAAATTATCTG |
NGG |
2 |
0.0308 |
Tier II |
| 5 |
NC_000014.9 |
14 |
70962922 |
- |
PCNX1 |
NNNCCCAATGAAATCATCTG |
NTG |
2 |
0.018 |
Tier II |
| 6 |
NC_000009.12 |
9 |
1339925 |
- |
LOC102723803 |
NNNCCCATTGAAATCATCTG |
NGG |
2 |
0.1978 |
Tier III |
| 7 |
NC_000008.11 |
8 |
48393143 |
- |
LOC105375821 |
NNNCCCACTGAAATCATCCG |
NAG |
2 |
0.1111 |
Tier III |
| 8 |
NC_000016.10 |
16 |
55460190 |
- |
MMP2-AS1 |
NNNCCCAATGAAATCAGCCC |
NGG |
2 |
0.0504 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
62411725 |
- |
Hkdc1 |
NNNCCCAATGAAATCACCCG |
NGG |
1 |
0.7059 |
Tier I |
| 2 |
NC_000082.6 |
16 |
4110608 |
+ |
Crebbp |
NNNCCCAATGAAAACATCCC |
NGG |
2 |
0.2653 |
Tier II |
| 3 |
NC_000080.6 |
14 |
118573527 |
+ |
Abcc4 |
NNNCCCTATGAAATCATCTG |
NGG |
2 |
0.2019 |
Tier II |
| 4 |
NC_000083.6 |
17 |
85250039 |
- |
Camkmt |
NNNCCCAATGAAAGCAACCG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000081.6 |
15 |
33632060 |
- |
4930592A05Rik |
NNNCCAAATGAAATCATCCA |
NGG |
2 |
0.8705 |
Tier III |
| 6 |
NC_000083.6 |
17 |
69423829 |
+ |
A930029G22Rik |
NNNTCCAATGAAATCATCCG |
NGG |
1 |
0.8 |
Tier III |
Other clones with same target sequence:
(none)
