Construct: sgRNA BRDN0001148025

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTCAGACAAGATGAAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM7 (54822)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76110

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 50611266 - TRPM7 NNNCAGACAAGATGAAACCA NGG 0 1.0 Tier I
2 NC_000019.10 19 49740064 - TSKS NNNCAGACAAGATGAAGCCA NAG 2 0.0458 Tier I
3 NC_000010.11 10 100543076 - HIF1AN NNNCAAACAAGAAGAAACCA NGG 2 0.6923 Tier II
4 NC_000009.12 9 89422825 - SEMA4D NNNCAGACATGATGGAACCA NGG 2 0.5735 Tier II
5 NC_000004.12 4 182764717 + TENM3 NNNGAGATAAGATGAAACCA NGG 2 0.4375 Tier II
6 NC_000013.11 13 100551333 + GGACT NNNCAGACAGGATAAAACCA NGG 2 0.4167 Tier II
7 NC_000003.12 3 4294682 - SUMF1 NNNCAGACAAGAAGAAACCT NGG 2 0.4154 Tier II
8 NC_000009.12 9 98543990 - GABBR2 NNNCAGACTAGAAGAAACCA NGG 2 0.4154 Tier II
9 NC_000004.12 4 6541067 + PPP2R2C NNNCAGACAAGAAGAAAACA NGG 2 0.3728 Tier II
10 NC_000004.12 4 149459226 + IQCM NNNCATACAGGATGAAACCA NGG 2 0.3704 Tier II
11 NC_000012.12 12 132282635 - GALNT9 NNNCCCACAAGATGAAACCA NGG 2 0.3409 Tier II
12 NC_000002.12 2 37321393 + PRKD3 NNNCAGACAAGGTGAAACAA NGG 2 0.3095 Tier II
13 NC_000006.12 6 124285133 - NKAIN2 NNNCAGTCAAGATGAAATCA NGG 2 0.2812 Tier II
14 NC_000022.11 22 46674144 - GRAMD4 NNNCAGACACGATGGAACCA NGG 2 0.2167 Tier II
15 NC_000002.12 2 204999392 - PARD3B NNNCAGACAGTATGAAACCA NGG 2 0.2137 Tier II
16 NC_000016.10 16 7342403 - RBFOX1 NNNCAGACAGGTTGAAACCA NGG 2 0.1852 Tier II
17 NC_000008.11 8 120504852 - MTBP NNNCAGAAAAGATGAAACCA NAG 2 0.1685 Tier II
18 NC_000015.10 15 79000661 - RASGRF1 NNNGAGACAAGATGAAACCA NAG 2 0.1296 Tier II
19 NC_000012.12 12 94992864 - NDUFA12 NNNCAGACAAGATAAAACGA NGG 2 0.0938 Tier II
20 NC_000003.12 3 14140318 - TMEM43 NNNCAGACACGAGGAAACCA NGG 2 0.087 Tier II
21 NC_000010.11 10 126336014 + ADAM12 NNNCAGACAAGATGAACCTA NGG 2 0.0814 Tier II
22 NC_000008.11 8 97112224 + CPQ NNNCAGACAAGATGAAAACA NTG 2 0.021 Tier II
23 NC_000002.12 2 29882272 + ALK NNNCAGACAAGAAGAAACCA NGC 2 0.0154 Tier II
24 NC_000009.12 9 121575982 + DAB2IP NNNCAGAGAAGATGAAACCA NGC 2 0.0143 Tier II
25 NC_000010.11 10 23038087 - ARMC3 NNNCAGACAAGATGAAACCA NAA 2 0.0 Tier II
26 NC_000023.11 X 51237740 + LOC105373204 NNNCAGACAAGAAAAAACCA NGG 2 0.5192 Tier III
27 NC_000016.10 16 80261867 - DYNLRB2-AS1 NNNCAGACGAGAAGAAACCA NGG 2 0.4615 Tier III
28 NC_000004.12 4 182764717 + LOC105377571 NNNGAGATAAGATGAAACCA NGG 2 0.4375 Tier III
29 NC_000006.12 6 111242393 + SLC60A2-DT NNNCAGAGAAGCTGAAACCA NGG 2 0.1692 Tier III
30 NC_000015.10 15 79000661 - LOC105370917 NNNGAGACAAGATGAAACCA NAG 2 0.1296 Tier III
31 NC_000015.10 15 44882694 - SORD2P NNNCAGACAAGGTGAACCCA NGG 2 0.1275 Tier III
32 NC_000004.12 4 25165357 + SEPSECS-DT NNNCAGACAAGATGAAACTA NAG 2 0.1197 Tier III
33 NC_000014.9 14 22105385 - TRA NNNCAGTCAAGATGAAACCA NAG 2 0.1134 Tier III
34 NC_000014.9 14 22105385 - TRAV24 NNNCAGTCAAGATGAAACCA NAG 2 0.1134 Tier III
35 NC_000016.10 16 63358497 + LOC105371308 NNNCAGACAACATGAAACCA NAG 2 0.1111 Tier III
36 NC_000008.11 8 97112224 + LOC101927066 NNNCAGACAAGATGAAAACA NTG 2 0.021 Tier III
37 NC_000018.10 18 67596494 - DSEL-AS1 NNNCAGACAAGATGAACCCA NTG 2 0.0069 Tier III
38 NC_000018.10 18 67596494 - LOC105372174 NNNCAGACAAGATGAACCCA NTG 2 0.0069 Tier III
39 NC_000010.11 10 23038087 - LOC107984215 NNNCAGACAAGATGAAACCA NAA 2 0.0 Tier III
40 NC_000011.10 11 78145693 + KCTD21-AS1 NNNCAGCCAAGATGACACCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 123501582 + Afg3l1 NNNCAGACAAGGCGAAACCA NGG 2 0.5702 Tier I
2 NC_000068.7 2 126826888 - Trpm7 NNNCAGACAGGATGAAACGA NGG 2 0.0694 Tier I
3 NC_000080.6 14 12757076 + Cadps NNNCAGACAAAATAAAACCA NGG 2 0.75 Tier II
4 NC_000071.6 5 134245414 - Gtf2i NNNAAGATAAGATGAAACCA NGG 2 0.7368 Tier II
5 NC_000071.6 5 66489695 + Apbb2 NNNCAAACAAGAAGAAACCA NGG 2 0.6923 Tier II
6 NC_000078.6 12 83368168 + Dpf3 NNNAAGACAAGAAGAAACCA NGG 2 0.583 Tier II
7 NC_000068.7 2 165445092 - Slc13a3 NNNCAGACATGATGAAATCA NGG 2 0.5672 Tier II
8 NC_000071.6 5 77049251 - Thegl NNNCAGACAAGATGGAAACA NGG 2 0.35 Tier II
9 NC_000072.6 6 37391701 - Creb3l2 NNNCAGAAAACATGAAACCA NGG 2 0.2786 Tier II
10 NC_000085.6 19 25161398 + Dock8 NNNCAGAAAAGATCAAACCA NGG 2 0.2786 Tier II
11 NC_000067.6 1 87516607 + Ngef NNNCAAACAAGATGAAACCA NAG 2 0.2593 Tier II
12 NC_000085.6 19 32229616 + Sgms1 NNNCTGACAAGAAGAAACCA NGG 2 0.2517 Tier II
13 NC_000077.6 11 49885910 - Mapk9 NNNCAGCCCAGATGAAACCA NGG 2 0.25 Tier II
14 NC_000082.6 16 91804161 - Itsn1 NNNCAGACAACATGAAACTA NGG 2 0.1978 Tier II
15 NC_000079.6 13 42896005 - Phactr1 NNNCAGACAAGAATAAACCA NGG 2 0.1846 Tier II
16 NC_000083.6 17 24492126 - Caskin1 NNNCAGACAAGCAGAAACCA NGG 2 0.1822 Tier II
17 NC_000068.7 2 72103160 - Rapgef4 NNNCAGACAAGTTGAAAACA NGG 2 0.1795 Tier II
18 NC_000076.6 10 24775697 - Enpp3 NNNCAGAAAAGAGGAAACCA NGG 2 0.1696 Tier II
19 NC_000071.6 5 96670327 - Fras1 NNNCAGACAAGAGGAAATCA NGG 2 0.1677 Tier II
20 NC_000085.6 19 46733760 - As3mt NNNCAGACAGGAGGAAACCA NGG 2 0.1449 Tier II
21 NC_000086.7 X 101312790 + Nlgn3 NNNCAGACAAGCTGAAAACA NGG 2 0.1417 Tier II
22 NC_000077.6 11 69782473 - Zbtb4 NNNCAGGCAAGATGAAGCCA NGG 2 0.1246 Tier II
23 NC_000072.6 6 23025977 + Ptprz1 NNNCAGACAAGATGAAACAA NAG 2 0.1111 Tier II
24 NC_000070.6 4 141018631 + Crocc NNNCTGACAAGAGGAAACCA NGG 2 0.0949 Tier II
25 NC_000076.6 10 29158962 + Soga3 NNNCAGACAAGATGAAAGTA NGG 2 0.0615 Tier II
26 NC_000067.6 1 193334024 - Lamb3 NNNCAGACAAGCTGAAGCCA NGG 2 0.0464 Tier II
27 NC_000069.6 3 85613765 - Gatb NNNCAGACAAGCTGAAAGCA NGG 2 0.0351 Tier II
28 NC_000072.6 6 73127747 + Dnah6 NNNCAGACAAGCTGAAAGCA NGG 2 0.0351 Tier II
29 NC_000074.6 8 14233169 - Dlgap2 NNNCAGACAAAATGAAACCA NGT 2 0.0161 Tier II
30 NC_000076.6 10 119654157 - Grip1 NNNCAGACAAGTTGAAACCA NTG 2 0.013 Tier II
31 NC_000080.6 14 26495952 + Slmap NNNCAGACAAGAAGAAACCA NGT 2 0.0112 Tier II
32 NC_000075.6 9 32705799 - Ets1 NNNCAGACAAGTTGAAACCA NGC 2 0.0074 Tier II
33 NC_000084.6 18 36192302 + Nrg2 NNNCAGACAAGAGGAAACCA NGC 2 0.0058 Tier II
34 NC_000085.6 19 57094994 - Ablim1 NNNCAGACAAGCTGAAACCA NGT 2 0.0042 Tier II
35 NC_000076.6 10 75761527 + Cabin1 NNNCAGACAAGATGACATCA NGG 2 0.0 Tier II
36 NC_000075.6 9 39131784 + Olfr27 NNNCAGACAAGATGACTCCA NGG 2 0.0 Tier II
37 NC_000076.6 10 127300630 + Mars1 NNNCAGACAGGATGACACCA NGG 2 0.0 Tier II
38 NC_000068.7 2 165445092 - Gm14437 NNNCAGACATGATGAAATCA NGG 2 0.5672 Tier III
39 NC_000075.6 9 68489656 + 4930502A04Rik NNNCAGTCGAGATGAAACCA NGG 2 0.2917 Tier III
40 NC_000085.6 19 8653855 + 9830166K06Rik NNNCAGACAAGAAGAATCCA NGG 2 0.0923 Tier III
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Other clones with same target sequence:

(none)