Construct: sgRNA BRDN0001148032
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAAAAATATGATGGACCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MARK2 (2011)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77590
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
63900028 |
+ |
MARK2 |
NNNAAAATATGATGGACCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
146719818 |
- |
ADGB |
NNNAAAATATGAAGGACCCT |
NGG |
2 |
0.4846 |
Tier II |
| 3 |
NC_000019.10 |
19 |
30006683 |
+ |
URI1 |
NNNAATATATGATGGACTCG |
NGG |
2 |
0.4592 |
Tier II |
| 4 |
NC_000001.11 |
1 |
100398370 |
- |
CDC14A |
NNNAAAATATGATGGACAAG |
NGG |
2 |
0.2308 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6112172 |
- |
Naaladl1 |
NNNCAAATATGATGGACCTG |
NGG |
2 |
0.1629 |
Tier I |
| 2 |
NC_000074.6 |
8 |
9350749 |
+ |
Fam155a |
NNNAAAATATGAAGGACCTG |
NGG |
2 |
0.3195 |
Tier II |
| 3 |
NC_000080.6 |
14 |
100180995 |
+ |
Klf12 |
NNNAAAATATGATCGACCTG |
NGG |
2 |
0.1978 |
Tier II |
| 4 |
NC_000071.6 |
5 |
89438850 |
+ |
Gc |
NNNAAAATATGATGCACCCT |
NGG |
2 |
0.1909 |
Tier II |
| 5 |
NC_000068.7 |
2 |
145177401 |
- |
Slc24a3 |
NNNAAAATCTGATGGACCCG |
NGA |
2 |
0.0397 |
Tier II |
Other clones with same target sequence:
(none)
